Dear Daniel,
This was my question also. I just purchased a new Mac book pro and see that
Mantiz sells an eGPU compatible with AMD cards for Thunderbolt 3 MacOS. I
would be very interested in anyone’s experience or advice in making stereo work
on the Mac platform.
Linda
On May 18, 2018, at
Dear Gloria,
This is the basic protocol we follow. Hope it helps
Arnaud Gautier and Marlon J. Hinner (eds.), Site-Specifi c Protein Labeling:
Methods and Protocols, Methods in Molecular Biology,
vol. 1266, DOI 10.1007/978-1-4939-2272-7_12, © Springer Science+Business Media
New York 2015
If your protein is His-tagged: spin down cells @~1500xg, adust pH with tris
buffer and NaOH to ~pH 7.1 (spin again higher speed if necessary to remove more
particulates) and pass over chemical resistant Ni-resin.
Linda Olson, PhD
Assistant Professor/
x-Ray Facility Manager
Dept. Biochemistry
