[ccp4bb] How to determine Quaternary symmetry

2017-07-10 Thread Vands
Hi , I have a protein which has A4 stoichiometry. I am trying to find symmetry component which should be D2 but one axis looks like translated which does not make it perfect D2. I am wondering if there is any tool which I can use to determine Quaternary symmetry. thanks --

[ccp4bb] NCS axis detrmination

2017-06-29 Thread Vands
Hi every one , I am looking for a tool to perfectly determine the NCS axis. I have two forms of crystals where diamer is with NCS but on a comparison of both forms, it looks like NCS axis is little off when i superpose two forms, i would like to measure rotation or

[ccp4bb] How to rotate subunits

2017-09-22 Thread Vands
HI all, I have a Structure of dimer where I would like to rotate one monomer with respect to other to know the interface surface area buried in different orientations. which software should I use to generate different orientation? thanks for the help. -- Vandna Kukshal

[ccp4bb] Non crystallography question

2017-12-08 Thread Vands
Hi All, Is there any Web server available where I can input my SAXS profile and it will give all closely matched PDB structures from the all available PDB. -- Vandna Kukshal Postdoctral Research Associate Dept. Biochemistry and Molecular Biophysics Washington University School of

Re: [ccp4bb] Non crystallography question

2017-12-08 Thread Vands
Sat, 9 Dec 2017 at 3:21 AM, Vands <vanx...@gmail.com> wrote: > >> Hi All, >>Is there any Web server available where I can input my SAXS >> profile and it will give all closely matched PDB structures from the all >> available PDB. >> >> >>

[ccp4bb] Resolution cut off

2018-02-12 Thread Vands
Hi, I solved a crystal structure at 1.69 A resolution with R /R free 18 / 20 i used 1.69 A data. Data completeness is 100 % and for the outer shell, it's 50 %. for i /Sig I > 1. Do I need to cut resolution in refinement?? Vandna Kukshal Postdoctral Research Associate Dept. Biochemistry

Re: [ccp4bb] Resolution cut off

2018-02-12 Thread Vands
.nih.gov/pmc/articles/PMC3689524/ > > For example > > On balance it’s unlikely throwing away measurements will make your model > better... > > Best wishes Graeme > > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of