Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-16 Thread A Leslie
I would like to thank Justin for his summary of this topic, which I'm sure many people found of interest, and is very much in the spirit of the bulletin board. I would just like to correct one factual error, in that it has been possible to specify anisotropic resolution limits to MOSFLM

Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-16 Thread Eleanor Dodson
I would like to have some comments on whether the maps before or after truncation are better . (obviously the Rfactors will be lower for the truncated data ..) I suspect it iwill be completely anecdotal - but I confess to a gut unhappiness about throwing out measurements.. eleanor Pavel

Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-16 Thread Pierre Rizkallah
Hi Everyone, I echo Andrew's thanks for the summary offered by Justin. I would like to mention another way to trim anisotropic diffraction patterns of the weak patches 'at source', as it were, in MOSFLM, by specifying a sigma cut off applied to each image. from the manual: RESOLUTION [ lowres

Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-16 Thread Harry Powell
Just to add my two ha'porth. I discussed this some years ago with Garib, just after I'd added the anisotropic cutoff to the resolution limits in Mosflm (mentioned by Andrew below); as I remember (and this is an invitation to Garib to contribute and correct me here!), the answer went along

[ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-15 Thread Justin Hall
Dear All; In response to my Anisotropic Diffraction In Refinement, which asked for suggestions for how best to proceed with refinement with an anisotropic data set, I received a large number of responses which overwhelmingly suggested using the UCLA Anisotropy Server

Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-15 Thread Peter Zwart
Application of a elliptical resolution boundary is justified because the resolution boundary from common integration programs (Denzo and Mosflm for example) is spherical where diffraction for anisotropic data is ellipsoidal. A spherical boundary would result in the inclusion of numerous poorly

Re: [ccp4bb] Summary for Anisotropic Diffraction In Refinement question

2009-09-15 Thread Pavel Afonine
This is why phenix.refine by default outputs both maps: 2mFo-DFc filled and not filled, and it is the best to look at both keeping in mind all pros and cons of each of them. Pavel. On 9/15/09 5:22 PM, Peter Zwart wrote: Application of a elliptical resolution boundary is justified because