Re: [ccp4bb] Using EMAN2.

[Tom Calcraft]

Hi Gurudatt,

If the alignment of the tilt series is already good enough (this also applies 
to .ali stacks from IMOD), you can run eman2 tomogram reconstruction using the 
—noali and —niter=0 options, which will just reconstruct your tilt series in 
their current alignment. Note that the tilt axis should lie along the Y axis in 
the aligned stacks.

Also, this question would be better addressed to the EMAN2 Google group 
(https://groups.google.com/g/eman2), or alternatively the 3dem or ccpem mailing 
lists.

Best regards,
Tom


On 22 Mar 2022, at 13:53, Gurudatt Patra 
mailto:gurudatt1...@gmail.com>> wrote:


External Sender: Use caution.

Dear All,

I was wondering if we can use the aligned tilt series that we get from Serial 
EM as raw data directly for reconstruction and particle picking in EMAN2.
Thank you so much in advance.

Sincerely,
Gurudatt



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[ccp4bb] Using EMAN2.

[Gurudatt Patra]

Dear All,

I was wondering if we can use the aligned tilt series that we get from
Serial EM as raw data directly for reconstruction and particle picking in
EMAN2.
Thank you so much in advance.

Sincerely,
Gurudatt



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https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1

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