gt; Thank you
> Dhiraj
>
>
> -
> From: CCP4 bulletin board on behalf of Nikolay
> Dobrev
> Sent: Thursday, February 3, 2022 11:53 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [External] Re: [ccp4bb] protease inhibitor and 3c protease
>
> Dear Dhi
Dobrev
Sent: Thursday, February 3, 2022 11:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [External] Re: [ccp4bb] protease inhibitor and 3c protease
Dear Dhiraj,
the 3C protease is from the Cysteine protease family.
Therefore for sure, you can add EDTA (if it is not indented for follow up IMAC
Dear Dhiraj,
the 3C protease is from the Cysteine protease family.
Therefore for sure, you can add EDTA (if it is not indented for follow up IMAC
purification) and that will inhibit most of the metalloprotease. Furthermore,
you can resort to Serine type protease inhibitors like PMSF or
Hi
sorry for off topic question. does anyone know which protease inhibitors we
can include safely while cleaving with 3C protease?
Thank you
Dhiraj
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Hi All,
Does any one add protease inhibitor to purified protein just before setting
trays?
If yes what is the typical % that should be added ?
regards
rashmi
Dear All,
I apologize if the questions has already been asked on this forum.
We are purifying a membrane that seems prone to proteolysis. Although we use
Protease Inhibitor cocktails during lysis and the first step of purification
we get rid of them after and only keep PMSF and EDTA as general
Regarding adding inhibitors. I'm not sure the effect on crystallization but if
you use too much you will get covalent modification of your protein from one of
them (I can check my notes as to which one). We searched many MS databases of
protein modifications to realize why our protein was the
