Hi,
I saw the same thing once and the cause was that the crystal had been
hideously over-exposed during data collection. As a result, essentially
all the spots at lower than 2.5A resolution were overloaded. The Wilson
plot was thus more or less flat at medium to high resolution and
accordingly the
On Mon, Dec 20, 2010 at 7:34 AM, Zhibing Lu billz...@gmail.com wrote:
Recently I solved a structure in which some water molecules have Bfactors
at 0 and overall wilson Bfactor is 0.654 based on PHENIX refinement. Is it
possible?
Very unlikely - it might be worth trying the latest nightly
some water molecules have Bfactors at 0
B-factors refining towards zero may be an indication of heavier molecules,
e.g. SO4. You have to model them manually.
Ralf
On 12/20/2010 10:34 AM, Zhibing Lu wrote:
Hi All,
Recently I solved a structure in which some water molecules have
Bfactors at 0 and overall wilson Bfactor is 0.654 based on PHENIX
refinement. Is it possible?
Bill Lu
Hi Bill,
What resolution are you working with here? An overall Wilson B
Hi Bill,
if you put a water oxygen in place where a heavier atom is, then water
oxygen's B-factor will refine to a value close to zero. This is the feature
that we currently use as one of many criteria to develop automatic
identification and building of metals.
Overall Wilson B-factor of 0.6A**2
