On 17/11/2022 19:03, Alessandro S. Nascimento wrote:
Dear all,
We are refining a 24-mer complex composed of 12 copies of protein A
and 12 copies of protein B. Since the resolution is not very high
(~3.0 Ang), we will probably want to copy the modifications made in
Coot for one chain to the ot
Hi Alessandro,
Wouldn't it be easier/simpler to use the modified chain together with its
(possibly) modified partner as a probe in MR to generate the completely
modified 12mer?
Just wondering.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben Gurion University
Beer Sheva, Israel
On Nov 17, 20
Hello, this sounds like a good case for electron density averaging which would
allow you to build one chain into the averaged density and then generate the
full set of 12 by NCS for refinement. Even better would be using the NCS in
refinement of the one chain you have built. Anyway, I would have
