Re: [ccp4bb] Protein-DNA complex crystallization

2011-11-21 Thread Francis E Reyes
Curious, how did you assess that your crystals only have DNA? F On Nov 21, 2011, at 8:59 AM, umar farook wrote: Dear All, I have been trying to crystallize protein DNA complex, but all the time i end up with DNA crystals. Even i changed the length of DNA many times but still no complex,

Re: [ccp4bb] Protein-DNA complex crystallization

2011-11-21 Thread sxn214
Sent from my Verizon Wireless Phone - Reply message - From: umar farook umarfaroo...@gmail.com Date: Mon, Nov 21, 2011 10:59 am Subject: [ccp4bb] Protein-DNA complex crystallization To: CCP4BB@JISCMAIL.AC.UK Dear All, I have been trying to crystallize protein DNA complex, but all the

Re: [ccp4bb] Protein-DNA complex crystallization

2011-11-21 Thread Phoebe Rice
What is Kd? Also, in reply to earlier posts: it is sadly common in crystallizing large protein-DNA complexes to go through a couple dozen different duplexes and several dismally-diffracting crystal forms before finding a good one. Phoebe From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on