Re: [ccp4bb] off topic: GPCR membane insertion/orientation

2011-03-04 Thread Van Den Berg, Bert
Hi Justin, I'm not sure if there are papers regarding this for GPCRs, but the phenomenon you're referring to is the positive inside rule. This basically means that the SecY translocon (in a way that is only partially clear) mediates membrane protein insertion in such a way that the (net)

Re: [ccp4bb] off topic: GPCR membane insertion/orientation

2011-03-04 Thread Pascal Egea
Hi Justin, Since GPCRs are polytopic a-helical transmembrane proteins, it is very likely that (1) insertion into the membrane is primarily performed by the Sec61 complex AKA translocon and (2) targeting to the membrane would be controlled by the signal recongition particle and its receptor. the

Re: [ccp4bb] off topic: GPCR membane insertion/orientation

2011-03-04 Thread Daniel Picot
In addition to Bert remarks, you can read this paper from the positive inside rule instigators. Seppälä S, Slusky JS, Lloris-Garcerá P, Rapp M, von Heijne G. Control of membrane protein topology by a single C-terminal residue. Science. 2010 Jun 25;328(5986):1698-700. Epub 2010 May 27. PubMed