---------- Forwarded message ---------- From: onetwo <twoon...@rediffmail.com> Date: 9 April 2015 at 19:48 Subject: [ccp4bb] visualizing .mrc maps in coot and model building To: ccp...@jiscmail.ac.uk
Dear All, This is regarding the protein structure model building using the density files or the .mrc file obtained by cryo-electron microscopy data. I want to seek some help on how to visualize these files in COOT. I am working on mac system and have recently installed CCP4 which has Coot- Dec 2014 version. When I load mrc file in coot via open map it gives error: map failed to open. Then using the em2em program, i have been successful in converting it to .ccp4 which I am able to open it in the coot. Please let me know if this the way I am supposed to do it. I have one more query that may be very naive but at which sigma level one should work towards these maps in coot ? I have read the paper by Brown et al, in ACTA D, 2015, they have mentioned the tools for macromolecular model building and refinement into electron cryo-microscopy and mentioned many useful tools in COOT like jiggle fit etc for this. So, if I can use ccp4 also in the same way like we do for crystallography and do refinement after model building in coot.For information, the protein I am working on has around 8A resolution. Looking forward for the help and guidance. Regards <https://sigads.rediff.com/RealMedia/ads/click_nx.ads/www.rediffmail.com/signatureline.htm@Middle?> Get your own *FREE* website, *FREE* domain & *FREE* mobile app with Company email. *Know More >* <http://track.rediff.com/click?url=___http://businessemail.rediff.com?sc_cid=sign-1-10-13___&cmp=host&lnk=sign-1-10-13&nsrv1=host>
