Re: [ECOLOG-L] Fear and Intimidation

2011-08-25 Thread Erin Chin
I think it depends on why one is using Ecolog. Personally, I use it as a source of current community activity/conferences/jobs to help me gauge for the future. I have also used it to ask questions about my own research and have received very helpful tips from students and professionals alike. I

Re: [ECOLOG-L] DNA extraction from feathers collected in the field

2011-07-29 Thread Erin Chin
Hi Ecologgers! Thank you so much for the advice and words of support!! I will try many of your suggestions in an attempt to increase yield. A little bit more detail about what I've done so far: nanodrop machine,PCR, and tissue extraction from preserved specimens. I use a nanodrop machine to

[ECOLOG-L] DNA extraction from feathers collected in the field

2011-07-26 Thread Erin Chin
Hello, I am a graduate student who is trying to extract DNA from feathers stored in ethanol (95%) collected from Costa's Hummingbirds (Calypte costae) and am getting very low yields. I am using the Dneasy Kit and the user-protocol for extracting DNA from the Qiagen website. My yields are