Hi, Ecologers, I asked about sampling air microbes several weeks ago. We have got many useful suggestions, thanks everyone!
Several people told me that they actually failed in getting workable samples for sequencing. I think this is a real situation to many people, and highlight the current challenge to this field. Several researchers pointed me to cyclonic air sampler. This is what we end up using in our current testing. I also got several suggestions about pass the air through a solution, but this is not quite portable for us. Of course passive sampling is among the most suggested. We have included this too in our plan for getting genome resources. For our own testing, we used a portable cyclonic air sampler to pass the air onto a filter paper placed on a petri dish. The filter paper was then extracted for DNA using a MO BIO water kit. In general, we did not get enough DNA for shotgun metagenomics, but kind of enough for amplicon sequencing. We are planning on a trial sequencing this month. I’ll keep update. Cheers, Zewei -- 宋泽伟 Zewei Song 华大生命科学研究院 宏基因组研究所 BGI-Shenzhen Institute of Metagenomics 电话 Tel: +86 13632929180 邮件 Email: songze...@genomics.cn 邮件 Email: songze...@outlook.com
