Re: [Felvtalk] Felvtalk - freezing interferon

2009-06-10 Thread Patricia . A . Elkins
Interferon is a protein.  It is dried into a powder which should be able 
to be frozen with
no problem.  But when proteins are dissolved into a liquid solution, 
freezing can be tricky.
As a scientist working with a variety of proteins, I can tell you that 
many proteins will start
to break down if frozen more than once.  And rapid freezing is preferable 
to slow freezing.
At home, the closest thing to rapid freezing could be done by putting the 
protein tube into ice in the back of
the freezer lowering
the temp more rapidly than if you just stick it on the shelf in the 
freezer.  Proteins also generally
survive freezing better if they are more dilute and freezing in smaller 
portions will allow the
freezing to go faster than freezing in big portions.

If a protein is degraded by freezing, it's structure starts to unwind. 
Some molecules of the
protein in the solution may degrade and others won't so you may still see 
that the protein has
some activity or it may lose all activity.  You might see some precipitate 
in the solution (always
a bad sign) or you might not.  Without some kind of activity readout, it 
seems impossible
to me to know whether freezing is causing a problem.

In summary, I would make up the solution to the appropriate dilution and 
freeze it in the smallest
portions that will work (need appropriate sized containers) in the coldest 
part of the freezer in ice.
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Re: [Felvtalk] Adam Please add to the CLS :(

2009-06-10 Thread catatonya
I'm so sorry Sherry.
tonya

--- On Sat, 5/30/09, Sherry DeHaan sherryd...@yahoo.com wrote:


From: Sherry DeHaan sherryd...@yahoo.com
Subject: [Felvtalk] Adam Please add to the CLS :(
To: Felvtalk felvtalk@felineleukemia.org
Date: Saturday, May 30, 2009, 8:53 PM


I am heavy hearted as we lost Adam yesterday.I will miss this handsome boy.he 
used to jump on my back while I was bent over scooping and my shirt would slide 
up,then he would put his cold feet on my bare back. :) He was an awesome sweet 
baby.I will miss you my Adam.
 


 
We who choose to surround ourselves with lives more temporary
than our own,
Live within a fragile circle,easily and often breached.
Unable to accept its awful gaps.
We still would have it no other way


      
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Re: [Felvtalk] Darcy Is An Angel Now

2009-06-10 Thread catatonya
I'm so sorry. :(
tonya

--- On Mon, 6/1/09, Jody Butler jbutler5...@bellsouth.net wrote:


From: Jody Butler jbutler5...@bellsouth.net
Subject: [Felvtalk] Darcy Is An Angel Now
To: felvtalk@felineleukemia.org felvtalk@felineleukemia.org
Date: Monday, June 1, 2009, 3:32 PM



Thank you all for the words of support. Sweet Darcy went downhill so fast, 
becoming unable to walk. I drove to an emergency clinic where they did their 
best for her through the  weekend. 

Yesterday we helped her to the Bridge, knowing there was just no more anyone 
could do. Her FeLv wouldn't let her fight off the ravages of whatever attacked 
her - probably a cancer that centered in her nervous system. 

We miss her dreadfully but know she had a good life with us, short though it 
was. She was much loved. 

Jody
Help blind cats see a future!
www.blindcatrescue.com



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Re: [Felvtalk] Freezing meds.....

2009-06-10 Thread Gloria B. Lane
but with what we get, freezing is easy and doesn't take up much  
freezer space - I've been doing it for a while, but haven't bought any  
in many months.  A friend just got some, I'll have to check the math  
with her.  I also don't see why the freezing would be a problem, but  
any thoughts/ opinion are appreciated.


Gloria



On Jun 9, 2009, at 5:24 PM, gary wrote:


Personally, I don't see why the amount of dilution should make any
difference in whether you can freeze it or not, but if you took a 3  
million
unit vial and diluted it to 30 units per ml, it would take up quite  
a lot of
freezer space as you would have one thousand, 100 ml containers to  
freeze

and store instead of 1000, one ml containers.

There is certainly plenty of CMA in the medical field.

Gary

-Original Message-
From: felvtalk-boun...@felineleukemia.org
[mailto:felvtalk-boun...@felineleukemia.org] On Behalf Of Lorrie
Sent: Tuesday, June 09, 2009 4:04 PM
To: felvtalk@felineleukemia.org
Subject: Re: [Felvtalk] Freezing meds.

Gary, we all count on you for info. on our vet meds, so thanks.

As for freezing stuff... I freeze all my meds, vet meds and
human meds.  I've had human meds in the freezer as long as ten years
and they still work fine.  I've also had Clavamox in the freezer for
two years past the expiration date and it works fine.

I've asked several pharmacists about freezing meds, and they all say
not to do it, BUT I wonder if they have to say this to cover their
butts just in case.  There is a lot of CMA in the medical field.

What are your thoughts on this?

Lorrie


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Re: [Felvtalk] Felvtalk - freezing interferon

2009-06-10 Thread Gloria B. Lane

All this is very helpful - thanks Patricia, MC, and Joel!

Gloria



On Jun 10, 2009, at 12:32 PM, patricia.a.elk...@gsk.com wrote:

Interferon is a protein.  It is dried into a powder which should be  
able

to be frozen with
no problem.  But when proteins are dissolved into a liquid solution,
freezing can be tricky.
As a scientist working with a variety of proteins, I can tell you that
many proteins will start
to break down if frozen more than once.  And rapid freezing is  
preferable

to slow freezing.
At home, the closest thing to rapid freezing could be done by  
putting the

protein tube into ice in the back of
the freezer lowering
the temp more rapidly than if you just stick it on the shelf in the
freezer.  Proteins also generally
survive freezing better if they are more dilute and freezing in  
smaller

portions will allow the
freezing to go faster than freezing in big portions.

If a protein is degraded by freezing, it's structure starts to unwind.
Some molecules of the
protein in the solution may degrade and others won't so you may  
still see

that the protein has
some activity or it may lose all activity.  You might see some  
precipitate

in the solution (always
a bad sign) or you might not.  Without some kind of activity  
readout, it

seems impossible
to me to know whether freezing is causing a problem.

In summary, I would make up the solution to the appropriate dilution  
and

freeze it in the smallest
portions that will work (need appropriate sized containers) in the  
coldest

part of the freezer in ice.
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[Felvtalk] Jack - please add to CLS

2009-06-10 Thread Kelley Saveika
First, can I say how annoyed I am with the neighbors?  I don't know if yall
remember, but Jack was the neighbor's tomcat we got neutered that was
pos/pos for FELV and FIV.  I try so hard to be charitable with people, and
am usually successful, and this person is in a wheelchair, but apparently
they saw Jack out in the yard not moving (but still alive) and brought him
in and put him on the back porch and he was there 3 days before he died.
 They didn't call me for assistance, or anything, I would have taken him to
the vet and put it on our burgeoning credit card bill.  Now they have new
kittens which we will have to take to get altered also, or it won't get
done...and she took a dog to the pound to be killed because he bit someone -
now she wants an Irish Setter puppy..sigh.   We have it in our code of
ethics that people are animals too, and should be treated well..but
sometimes it is just hard.
Anyway, please add Jack to the CLS...may he have a better life over the
bridge than he did here, and may he forgive me for not doing anything to
help him, I would have done so if I had known.

Kelley

-- 
Rescuties - Saving the world, one cat at a time.

http://www.rescuties.org

Vist the Rescuties stores and save a kitty life!

http://www.amazon.com/exec/obidos/redirect-home?tag=rescuties-20

http://www.zazzle.com/rescuties*

Buy or renew magazines and help our kitties!
http://www.magfundraising.com/rescuties

Help us spay some kitties!

http://rescuties.chipin.com/feed-hungry-animals

Rather than helping, it's easier to point fingers and say take  them first
as long as you leave me alone.
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Re: [Felvtalk] Jack - please add to CLS

2009-06-10 Thread Diane Rosenfeldt
Oh, Lord, Kelley, these people really have no right to any pets!!!  Poor
Jack, I hope he didn't suffer too much.  I know he knows you cared and would
have helped him.  The bigger question is whether he can forgive his
boneheaded people.  Gentle Bridge vibes to the dear boy.

I know you're trying to be charitable, but do you know if they fed and
watered Jack during that time?  For his sake I pray they did, but if they
didn't, doesn't that constitute abuse, and could they not be reported?  Or
would that open a can of worms with your own situation that you'd rather
not?

Diane R.

-Original Message-
From: felvtalk-boun...@felineleukemia.org
[mailto:felvtalk-boun...@felineleukemia.org] On Behalf Of Kelley Saveika
Sent: Thursday, June 11, 2009 12:41 AM
To: felvtalk
Subject: [Felvtalk] Jack - please add to CLS

First, can I say how annoyed I am with the neighbors?  I don't know if yall
remember, but Jack was the neighbor's tomcat we got neutered that was
pos/pos for FELV and FIV.  I try so hard to be charitable with people, and
am usually successful, and this person is in a wheelchair, but apparently
they saw Jack out in the yard not moving (but still alive) and brought him
in and put him on the back porch and he was there 3 days before he died.
 They didn't call me for assistance, or anything, I would have taken him to
the vet and put it on our burgeoning credit card bill.  Now they have new
kittens which we will have to take to get altered also, or it won't get
done...and she took a dog to the pound to be killed because he bit someone -
now she wants an Irish Setter puppy..sigh.   We have it in our code of
ethics that people are animals too, and should be treated well..but
sometimes it is just hard.
Anyway, please add Jack to the CLS...may he have a better life over the
bridge than he did here, and may he forgive me for not doing anything to
help him, I would have done so if I had known.

Kelley

--
Rescuties - Saving the world, one cat at a time.

http://www.rescuties.org

Vist the Rescuties stores and save a kitty life!

http://www.amazon.com/exec/obidos/redirect-home?tag=rescuties-20

http://www.zazzle.com/rescuties*

Buy or renew magazines and help our kitties!
http://www.magfundraising.com/rescuties

Help us spay some kitties!

http://rescuties.chipin.com/feed-hungry-animals

Rather than helping, it's easier to point fingers and say take  them first
as long as you leave me alone.
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Re: [Felvtalk] Jack - please add to CLS

2009-06-10 Thread Gloria B. Lane
Oh, Kelley, that's awful.  Sleep soft sweet Jack, I'm sorry for the  
suffering you endured.


Gloria



On Jun 11, 2009, at 12:40 AM, Kelley Saveika wrote:

First, can I say how annoyed I am with the neighbors?  I don't know  
if yall

remember, but Jack was the neighbor's tomcat we got neutered that was
pos/pos for FELV and FIV.  I try so hard to be charitable with  
people, and
am usually successful, and this person is in a wheelchair, but  
apparently
they saw Jack out in the yard not moving (but still alive) and  
brought him
in and put him on the back porch and he was there 3 days before he  
died.
They didn't call me for assistance, or anything, I would have taken  
him to
the vet and put it on our burgeoning credit card bill.  Now they  
have new
kittens which we will have to take to get altered also, or it won't  
get
done...and she took a dog to the pound to be killed because he bit  
someone -

now she wants an Irish Setter puppy..sigh.   We have it in our code of
ethics that people are animals too, and should be treated well..but
sometimes it is just hard.
Anyway, please add Jack to the CLS...may he have a better life over  
the
bridge than he did here, and may he forgive me for not doing  
anything to

help him, I would have done so if I had known.

Kelley

--
Rescuties - Saving the world, one cat at a time.

http://www.rescuties.org

Vist the Rescuties stores and save a kitty life!

http://www.amazon.com/exec/obidos/redirect-home?tag=rescuties-20

http://www.zazzle.com/rescuties*

Buy or renew magazines and help our kitties!
http://www.magfundraising.com/rescuties

Help us spay some kitties!

http://rescuties.chipin.com/feed-hungry-animals

Rather than helping, it's easier to point fingers and say take   
them first

as long as you leave me alone.
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