[Felvtalk] slight positives v very very very positives?

2008-07-19 Thread MaryChristine
there's no such thing as very very very, or very very or very positive, or
faint positive: there is positive, or there is negative. give me a couple of
days and i will even be able to give you the reference from a far more
reputable source than myself, because i just found it for sure the other
day. i believe the wording, as some of us have less elegantly stated it
before, was that the spot responds with coloration, or no coloration. if
you've never seen a snap-type ELISA test, it might not make that much sense,
but it's a little piece of paper that has dots in different places that are
treated to respond to different antigens or antibodies or test solution: it
doesn't measure how MUCH, or how RECENT, or any of that, it just says that
yes, that particular whatever IS there. i have never seen, anywhere, any
reference to the color having any quantifiable value, especially considering
the nature of the test: you HAVE to read the dots at a specific time period
after the test is started, or it is not valid, as the paper can continue to
react past that time. so if the FeLV circle has NO COLOR at 10 minutes or
whatever the specific test specifies, the test result is NEGATIVE. ten
minutes later, for those vets who forgot to set the timer (doing snap tests
incorrectly is one of the top ten mistakes vets admit to) that same circle
may actually show color. the cat is NOT positiive, because the accurate
testing period is OVER. so a light spot at 10 minutes is as positive as a
dark spot at 10 minutes is, either -- it's the existence of color, at the
specific time that counts.

do vets not realize this? i'm not sure. i don't remember the last time i
read the idexx instructions, but they're pretty detailed, and i don't think
they were unclear. so i'm not sure if vets are trying to cushion the blow,
knowing that one should never take the results of a single test, especially
an ELISA, as the final determinant, they haven't read the instructions
themselves in years, or well, it LOOKS lighter, and they've just forgotten
whatever they knew about the underlying science of the test.

.





On Wed, Jul 16, 2008 at 10:21 AM, Sharyl [EMAIL PROTECTED] wrote:

 I have been feeding a dumpster colony of kitties for several months with
 the goal of doing TNR (trap/neuter/release) for those that I can not
 rescue.  Of the 3 kittens I rescued 2, Sissy and Rocket, were FeLV+.  They
 are about 9 months old now and are doing well.  Both have test positive
 twice.



 Their sister, Daisy, had a litter before I was able to TNR her.  The
 kittens were approx. 4 weeks old when I got them.  I took them because 1 had
 both eyes stuck shut and 1 had 1 eye stuck shut.  The next day I was finally
 able to trap Daisy using a kitten as bait in the carrier.Daisy has since
 been spayed and released.  The babies have received one worm treatment, got
 terramycin salve in their eyes for a week, and have lysine added to their
 food.



 It appears that Mattie will be blind.  She is the biggest of the 4 and very
 feisty.   I had her tested and the vet said she was very, very, very FeLV+.
 No idea what 3 verys mean.  CJ may have some limited vision in her bad eye.
 Bright Eyes and Houdini (escaped from the dog crate twice now) seem healthy.
   I am adding L-Lysine and Mega C Plus to their food.  I am looking for
 advice on what else I can do to help them throw off this virus.



 They will each be tested when I have them spayed.   Sissy and Rocket were
 several months old when I rescued them.  Didn't know if starting treatments
 earlier would improve the odds that they could beat this.   Any advise on
 how to proceed with now 5 1/2 wk old kittens would be appreciated.



 By the way Stormie, same colony but different Mom from Sissy, Rocket and
 Daisy, tested negative.  I did not have Daisy tested.  I knew I could not
 afford to keep her if she was positive and simply do not have the
 disposition to have a cat PTS simply because she is positive.   At least she
 will not have any more litters.  There is one more sister, Mae, that I have
 been trying to trap for TNR.

 Sharyl Sissy, Rocket and the babies


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Re: [Felvtalk] slight positives v very very very positives?

2008-07-19 Thread TatorBunz
 
 
I totally agree with you on this MC
It's either positive or negative!
 
In a message dated 7/19/2008 11:54:02 A.M. Pacific Daylight Time,  
[EMAIL PROTECTED] writes:

there's  no such thing as very very very, or very very or very positive, or 
faint  positive: there is positive, or there is negative. give me a couple of 
days  and i will even be able to give you the reference from a far more 
reputable  source than myself, because i just found it for sure the other day. 
i 
believe  the wording, as some of us have less elegantly stated it before, was 
that 
the  spot responds with coloration, or no coloration. if you've never seen a  
snap-type ELISA test, it might not make that much sense, but it's a little  
piece of paper that has dots in different places that are treated to respond  
to different antigens or antibodies or test solution: it doesn't measure how  
MUCH, or how RECENT, or any of that, it just says that yes, that particular  
whatever IS there. i have never seen, anywhere, any reference to the color  
having any quantifiable value, especially considering the nature of the test:  
you 
HAVE to read the dots at a specific time period after the test is started,  
or it is not valid, as the paper can continue to react past that time. so if  
the FeLV circle has NO COLOR at 10 minutes or whatever the specific test  
specifies, the test result is NEGATIVE. ten minutes later, for those vets who  
forgot to set the timer (doing snap tests incorrectly is one of the top ten  
mistakes vets admit to) that same circle may actually show color. the cat is  
NOT 
positiive, because the accurate testing period is OVER. so a light spot at  10 
minutes is as positive as a dark spot at 10 minutes is, either -- it's the  
existence of color, at the specific time that counts. 

do vets not  realize this? i'm not sure. i don't remember the last time i 
read the idexx  instructions, but they're pretty detailed, and i don't think 
they 
were  unclear. so i'm not sure if vets are trying to cushion the blow, 
knowing that  one should never take the results of a single test, especially an 
ELISA, as  the final determinant, they haven't read the instructions themselves 
in 
years,  or well, it LOOKS lighter, and they've just forgotten whatever they 
knew about  the underlying science of the test.





TAZZY'S ANIMAL TRANSPORTS
SIAMESE  COLLIE RESCUE
_http://tazzys.org/_ (http://tazzys.org/index.html) 


_http://www.hurricanepetsrescue.org/_ (http://www.hurricanepetsrescue.org/) 

OTRA VERIFIED TRANSPORTER
(On The Road  Again)

Terrie  Mohr-Forker




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