[galaxy-dev] QUAL file format(s)

2011-03-03 Thread Peter Cock
Hi all,

Is there any documentation on what Galaxy means by the different qual formats?

In particular why is there a qual454 and not a qualsanger (to match fastq)?

Historically QUAL files were used back in Sanger/capillary sequencing
by PHRED, and just hold PHRED scores as integers. This was followed
by the Roche 454 output.

Peter
___
To manage your subscriptions to this and other Galaxy lists, please use the 
interface at:

  http://lists.bx.psu.edu/


Re: [galaxy-dev] rpy and R

2011-03-03 Thread Nate Coraor
Sarah Diehl wrote:
 Hi all,
 
 I'm currently working on setting up a fresh Galaxy server at our
 institute. I'm going through the tool dependencies list and install
 everything on it. I installed the latest R version (2.12.2) and now
 I'm trying to install rpy. With some workarounds I managed to
 compile rpy, but when I try to import it in python, I get the
 following error:
 
  import rpy
 Traceback (most recent call last):
   File stdin, line 1, in module
   File rpy.py, line 134, in module
  % RVERSION)
 RuntimeError: No module named _rpy2122
 
   RPy module can not be imported. Please check if your rpy
   installation supports R 2.12.2. If you have multiple R versions
   installed, you may need to set RHOME before importing rpy. For
   example:
 
from rpy_options import set_options
set_options(RHOME='c:/progra~1/r/rw2011/')
from rpy import *
 
 It looks like my version of R isn't supported by rpy. Does anybody
 know which version of R still works with rpy? I couldn't find
 anything about it. What about rpy2? Is it not supported by Galaxy?

Hi Sarah,

Although I haven't tested with 2.12.2, I know it works with at least
2.11.0.  The error above is indicating that rpy is installed somewhere
on python's path but the C-extension module for the version of R found
on your $PATH (2.12.2) is not present.  Most likely this is because rpy
was already installed, and is being found earlier on python's path than
the version of rpy that you installed.  The older rpy was built against
a different version of R.

If you find the original installation of rpy you should be able to
determine from _rpyversion.so what version of R it was built against,
and put that version of R ahead of 2.12.2 in your $PATH.

Alternatively, you can find and uninstall the old version of rpy, which
should allow your newer version to be found and used.

python's path can be determined with:

  python -c 'import sys; print \n.join( sys.path )'

There is some effort underway in the community to rewrite rpy tools to
use rpy2, but this work is not yet complete.

Hope this helps,
--nate

 
 Thanks very much in advance for your help!
 
 Best regards,
 Sarah Diehl
 ___
 To manage your subscriptions to this and other Galaxy lists, please use the 
 interface at:
 
  http://lists.bx.psu.edu/
___
To manage your subscriptions to this and other Galaxy lists, please use the 
interface at:

  http://lists.bx.psu.edu/


Re: [galaxy-dev] rpy and R

2011-03-03 Thread Sarah Diehl

Hi Nate,

thank you very much for your help! I managed to figure out the problem. 
There seemed to be an old version of R and somehow (I still don't know 
how) the rpy installation did only find the old one. I hard-coded the 
directory of the new R in the setup.py (everything else failed) and then 
it compiled against the new R version.


Best regards,
Sarah


On 03/03/2011 05:11 PM, Nate Coraor wrote:

Sarah Diehl wrote:

Hi all,

I'm currently working on setting up a fresh Galaxy server at our
institute. I'm going through the tool dependencies list and install
everything on it. I installed the latest R version (2.12.2) and now
I'm trying to install rpy. With some workarounds I managed to
compile rpy, but when I try to import it in python, I get the
following error:


import rpy

Traceback (most recent call last):
   File stdin, line 1, inmodule
   File rpy.py, line 134, inmodule
  % RVERSION)
RuntimeError: No module named _rpy2122

   RPy module can not be imported. Please check if your rpy
   installation supports R 2.12.2. If you have multiple R versions
   installed, you may need to set RHOME before importing rpy. For
   example:

 from rpy_options import set_options
 set_options(RHOME='c:/progra~1/r/rw2011/')
 from rpy import *

It looks like my version of R isn't supported by rpy. Does anybody
know which version of R still works with rpy? I couldn't find
anything about it. What about rpy2? Is it not supported by Galaxy?


Hi Sarah,

Although I haven't tested with 2.12.2, I know it works with at least
2.11.0.  The error above is indicating that rpy is installed somewhere
on python's path but the C-extension module for the version of R found
on your $PATH (2.12.2) is not present.  Most likely this is because rpy
was already installed, and is being found earlier on python's path than
the version of rpy that you installed.  The older rpy was built against
a different version of R.

If you find the original installation of rpy you should be able to
determine from _rpyversion.so what version of R it was built against,
and put that version of R ahead of 2.12.2 in your $PATH.

Alternatively, you can find and uninstall the old version of rpy, which
should allow your newer version to be found and used.

python's path can be determined with:

   python -c 'import sys; print \n.join( sys.path )'

There is some effort underway in the community to rewrite rpy tools to
use rpy2, but this work is not yet complete.

Hope this helps,
--nate



Thanks very much in advance for your help!

Best regards,
Sarah Diehl
___
To manage your subscriptions to this and other Galaxy lists, please use the 
interface at:

  http://lists.bx.psu.edu/




___
To manage your subscriptions to this and other Galaxy lists, please use the 
interface at:

 http://lists.bx.psu.edu/


[galaxy-dev] Fwd: [Bosc] Bioinformatics Open Source Conference (BOSC 2011)--Call for Abstracts

2011-03-03 Thread Peter Cock
Hopefully this will be of interest to some of you...

-- Forwarded message --
From: Nomi Harris nlhar...@lbl.gov
Date: Thu, Mar 3, 2011 at 7:37 PM
Subject: [Bosc] Bioinformatics Open Source Conference (BOSC
2011)--Call for Abstracts
To: bosc-annou...@lists.open-bio.org, memb...@open-bio.org, GMOD
Announcements List gmod-annou...@lists.sourceforge.net, GMOD
Developers List gmod-de...@lists.sourceforge.net
Cc: Nomi Harris nlhar...@lbl.gov


We invite you to submit an abstract to BOSC 2011!  Please forward this
message as appropriate, and forgive multiple postings.

Call for Abstracts for the 12th Annual Bioinformatics Open Source
Conference (BOSC 2011)
An ISMB 2011 Special Interest Group (SIG)

Dates: July 15-16, 2011
Location: Vienna, Austria
Web site: http://www.open-bio.org/wiki/BOSC_2011
Email: b...@open-bio.org
BOSC announcements mailing list:
http://lists.open-bio.org/mailman/listinfo/bosc-announce

Important Dates:
April 18, 2011: Deadline for submitting abstracts to BOSC 2011
May 9, 2011: Notifications of accepted abstracts emailed to
corresponding authors
July 13-14, 2011: Codefest 2011 programming session (see
http://www.open-bio.org/wiki/Codefest_2011 for details)
July 15-16, 2011: BOSC 2011
July 17-19, 2011: ISMB 2011

The Bioinformatics Open Source Conference (BOSC) is sponsored by the
Open Bioinformatics Foundation (O|B|F), a non-profit group dedicated
to promoting the practice and philosophy of Open Source software
development within the biological research community. To be considered
for acceptance, software systems representing the central topic in a
presentation submitted to BOSC must be licensed with a recognized Open
Source License, and be freely available for download in source code
form.

We invite you to submit abstracts for talks and posters.  Sessions include:
- Approaches to parallel processing
- Cloud-based approaches to improving software and data accessibility
- The Semantic Web in open source bioinformatics
- Data visualization
- Tools for next-generation sequencing
- Other Open Source software

In addition to the above sessions, there will be a panel discussion
about Meeting the challenges of inter-institutional collaboration.
We are also working to arrange a joint session with one of the other
ISMB SIGs.

Thanks to generous sponsorship from Eagle Genomics and an anonymous
donor, we are pleased to announce a competition for three Student
Travel Awards for BOSC 2011. Each winner will be awarded $250 to
defray the costs of travel to BOSC 2011.

For instructions on submitting your abstract, please visit
http://www.open-bio.org/wiki/BOSC_2011#Abstract_Submission_Information

BOSC 2011 Organizing Committee:
Nomi Harris and Peter Rice (co-chairs); Brad Chapman, Peter Cock,
Erwin Frise, Darin London, Ron Taylor


___
BOSC mailing list
b...@lists.open-bio.org
http://lists.open-bio.org/mailman/listinfo/bosc

___
Please keep all replies on the list by using reply all
in your mail client.  To manage your subscriptions to this
and other Galaxy lists, please use the interface at:

  http://lists.bx.psu.edu/