Re: [galaxy-dev] two versions of show datasets API call?

2013-03-18 Thread Jeremy Goecks
This is a using-the-API question; not sure if it belongs in galaxy-dev or galaxy-user ! galaxy-dev is best because it's dealing with the development of Galaxy. There seem to be two ways to retrieve metadata for a dataset, one in the Histories API and one in the Datasets API. They return

Re: [galaxy-dev] two versions of show datasets API call?

2013-03-18 Thread Jeremy Goecks
Historical reasons only; this should be fixed. And this is now fixed. Take a look at these changesets for details: https://bitbucket.org/galaxy/galaxy-central/commits/579c0b64e463918691bb38e718af8d70dd6f9fa7

Re: [galaxy-dev] Search software on public Galaxy servers

2013-03-04 Thread Jeremy Goecks
The tools API, incomplete as it is, could be used to automatically generate a list of tools for a single instance. J. On Mar 4, 2013, at 5:37 AM, Hans-Rudolf Hotz wrote: On 03/03/2013 05:18 PM, Dave Clements wrote: Hi Bernardo, I'm intrigued by this idea. It might be possible to

Re: [galaxy-dev] cuff configure

2013-03-03 Thread Jeremy Goecks
To perform bias correction using sequence data in Cufflinks/Cuffcompare/Cuffdiff, you'll need to set up and add entries to sam_fa_indices.loc Of course, this isn't the most intuitive place, and we're planning to improve loc file structure and usage in the future. Best, J. On Mar 1, 2013, at

Re: [galaxy-dev] bowtie-wrapper

2013-02-28 Thread Jeremy Goecks
again Best wishes, Peter On 27/02/13 18:33, Jeremy Goecks wrote: Thanks Peter. I've added your patch to the Trello card tracking incorporation of community contributions to the Bowtie wrapper: https://trello.com/c/TdYxdbkm Best, J. On Feb 27, 2013, at 4:35 AM, Peter Briggs wrote

Re: [galaxy-dev] bowtie-wrapper

2013-02-28 Thread Jeremy Goecks
Attached to the card, thanks Peter. J. On Feb 28, 2013, at 11:14 AM, Peter Briggs wrote: Hi Jeremy No problem - zip file with the two patches is attached, please let me know if there's a problem. Best wishes Peter On 28/02/13 15:07, Jeremy Goecks wrote: Hmm, I don't see a patch

Re: [galaxy-dev] bowtie-wrapper

2013-02-27 Thread Jeremy Goecks
Thanks Peter. I've added your patch to the Trello card tracking incorporation of community contributions to the Bowtie wrapper: https://trello.com/c/TdYxdbkm Best, J. On Feb 27, 2013, at 4:35 AM, Peter Briggs wrote: Hello Alexander I've made some changes to our local copy of the bowtie

[galaxy-dev] Implementing dataset collections

2013-02-27 Thread Jeremy Goecks
what you described be accepted? Any thoughts you or anyone has on the future direction of composite datatypes in general would be appreciated? Thanks for your time, -John On Fri, Oct 12, 2012 at 2:44 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Hi Jim, This is nice

Re: [galaxy-dev] Installing Prebuilt Indexes for Tophat for Illumina - What to do for color space files?

2013-02-20 Thread Jeremy Goecks
I see where I would use the *.ebwt files in the bowtie_index.loc file. But I'm not sure what to reference in the bowtie_index_color.loc file? (The same *.ebwt files??) You need to download the colorspace indices separately: http://bowtie-bio.sourceforge.net/index.shtml Good luck,

Re: [galaxy-dev] Test data for tophat2 with hg19 reference genome

2013-02-20 Thread Jeremy Goecks
There is some test data for hg19 on the RNA-seq exercise: https://main.g2.bx.psu.edu/rna-seq Best, J. On Feb 20, 2013, at 6:47 AM, Sachit Adhikari wrote: Hi, I need a test_data for Tophat with the hg19 reference genome? The test_data from this website

Re: [galaxy-dev] tophat for illumina looking in wrong directory for bowtie2 indexes?

2013-02-20 Thread Jeremy Goecks
But this is what's in my galaxy-dist/tool-data/bowtie2_indices.loc file: mm10mm10mm10 /usr/local/galaxy/indexes/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index You need: /usr/local/galaxy/indexes/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/genome J. Why isn't it looking in that

Re: [galaxy-dev] [galaxy-user] Uploading large archive histories

2013-02-18 Thread Jeremy Goecks
After discussing this with Getiria, I think what is needed here is a way to use FTP to upload compressed histories from the import screen as is available from the upload data tool. The problem we're currently experiencing is that when we try to upload a compressed history where the

Re: [galaxy-dev] hg19 reference gnome for Tophat2

2013-02-15 Thread Jeremy Goecks
, I was successfully able to run the test data from the tophat2 website in command line. I think there's something wrong with the reference genome. I have correctly specified the path of reference genome? What's the problem I am facing? On Thu, Feb 14, 2013 at 1:44 PM, Jeremy Goecks

Re: [galaxy-dev] Error while writing data in tophat2

2013-02-15 Thread Jeremy Goecks
This is not a known bug. For questions related to Tophat errors, you might try the Tophat mailing list: tophat.cuffli...@gmail.com Good luck, J. On Feb 15, 2013, at 6:02 AM, Sachit Adhikari wrote: Galaxy is working fine in command line whereas in Galaxy at last i got this error:

Re: [galaxy-dev] hg19 reference gnome for Tophat2

2013-02-14 Thread Jeremy Goecks
the same problem and regarding settings I haven't changed a thing of Tophat2. What might be causing this error? Thanks On Wed, Feb 13, 2013 at 7:10 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: If the other outputs are fine, then the problem is likely with your data and/or your

Re: [galaxy-dev] Why are the search options in datasets no longer using AND logic?

2013-02-14 Thread Jeremy Goecks
This is a bug, and it's been fixed in this changeset: https://bitbucket.org/galaxy/galaxy-central/commits/db08c095de0c249f3a6cde62f254c104de1fb6ea Thanks for pointing it out, J. On Feb 13, 2013, at 8:25 PM, Anthonius deBoer wrote: Hi, One very cool feature was to be able to search

Re: [galaxy-dev] hg19 reference gnome for Tophat2

2013-02-13 Thread Jeremy Goecks
Shall I replace: /orig/path/hg19hg19hg19 /depot/data2/galaxy/bowtie2/hg19/hg19 with hg19 hg19hg19 Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index Yes, that's correct. Finally, please email only galaxy-dev with questions about local installations;

Re: [galaxy-dev] hg19 reference gnome for Tophat2

2013-02-13 Thread Jeremy Goecks
Also, do I have make all the reference files executable? No, that's not necessary. Best, J. ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists,

Re: [galaxy-dev] Error executing tophat2

2013-02-13 Thread Jeremy Goecks
Error in tophat: [2013-02-13 02:02:43] Beginning TopHat run (v2.0.7) --- [2013-02-13 02:02:43] Checking for Bowtie Bowtie version:2.0.6.0 [2013-02-13 02:02:43] Checking for Samtools Samtools version:

Re: [galaxy-dev] hg19 reference gnome for Tophat2

2013-02-13 Thread Jeremy Goecks
? Thanks, Sachit On Wed, Feb 13, 2013 at 1:20 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Shall I replace: /orig/path/hg19hg19hg19 /depot/data2/galaxy/bowtie2/hg19/hg19 with hg19 hg19hg19 Homo_sapiens/UCSC/hg19/Sequence

Re: [galaxy-dev] Circster save just hangs

2013-02-13 Thread Jeremy Goecks
I will try to reproduce. In the meantime, can you please check the JavaScript console and forward along any errors that you see? Thanks, J. On Feb 12, 2013, at 11:15 PM, Anthonius deBoer wrote: Hi, I had created a trackster vizualization that had not finished indexing yet,

Re: [galaxy-dev] Extract genomic DNA job error

2013-02-12 Thread Jeremy Goecks
So we did the following: Downloaded the data Added the paths to .loc file restarted galaxy We still are getting the following error: 2: Extract Genomic DNA on data 1 empty format: fasta, database: hg19 56 warnings, 1st is: Chromosome by name 'chr22' was not found for build 'hg19'.

Re: [galaxy-dev] Extract genomic DNA job error

2013-02-08 Thread Jeremy Goecks
Hi Ricardo, This tool requires external data that you'll need to configure. Here's how: (1) download the 2bit file from UCSC for your genomes into a local directory from here: http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/ (2) add an entry like this to alignseq.loc (note that you'll

Re: [galaxy-dev] Import history from web server to local server

2013-02-07 Thread Jeremy Goecks
is the problem? I have certainly mis-understanded your explaination. Thanks. julie 2013/2/6 Jeremy Goecks jeremy.goe...@emory.edu Unfortunately using wget won't work in this case. The reason you have access to the history is your Galaxy cookie, which isn't shared with wget/curl. You'll

Re: [galaxy-dev] Import history from web server to local server

2013-02-06 Thread Jeremy Goecks
This is likely a permissions issue: importing your history into another Galaxy instance requires the history to be accessible. Here's a solution that, while ugly, should work: (1) Make the history accessible by going to Share/Publish and clicking the option to make it accessible. (2) Export

Re: [galaxy-dev] Import history from web server to local server

2013-02-06 Thread Jeremy Goecks
work. I have the same error. Sorry and thanks for the creation of the card. Julie 2013/2/6 Jeremy Goecks jeremy.goe...@emory.edu This is likely a permissions issue: importing your history into another Galaxy instance requires the history to be accessible. Here's a solution that, while ugly

Re: [galaxy-dev] Import history from web server to local server

2013-02-06 Thread Jeremy Goecks
with html code thanks again. Julie 2013/2/6 Jeremy Goecks jeremy.goe...@emory.edu Ah, I see the issue: the Cistrome instance cannot be used anonymously (without login). It's not possible for one Galaxy instance to work with another instance's history because instances work with objects

Re: [galaxy-dev] Divide the web interface into sections for a tool.

2013-02-06 Thread Jeremy Goecks
Hi Luobin, This isn't yet possible but is planned: https://trello.com/c/KxlQK0FB Best, J. On Feb 6, 2013, at 2:47 PM, Luobin Yang wrote: Hi, Galaxy developers, I would like to have the parameters on the web interface to be packed into sections for a tool. Is there a way to do this? There

Re: [galaxy-dev] HTML output, hide preview text box?

2013-02-04 Thread Jeremy Goecks
My outputs section in the tool's xml file is: outputs data format=HTML name=fred_report label=generating FRED report.../ /outputs You should set the output format to 'html' — all lower case. Galaxy has a list of known dataset types in datatypes.conf, and your output format should

Re: [galaxy-dev] HTML output, hide preview text box?

2013-02-03 Thread Jeremy Goecks
All works great, but I was hoping that there's an easy way to remove the preview text box from the resulting History pane item (it currently shows the unrendered html tags)? This should not be happening. If your tool's output dataset has type HTML, the peek (preview) should say 'HTML file'

Re: [galaxy-dev] Patch for RepeatMasker.xml for RepeatMasker 3.3.0

2013-01-31 Thread Jeremy Goecks
On Jan 31, 2013, at 8:15 PM, Guest, Simon wrote: Dear Galaxians, We are using Galaxy with RepeatMasker 3.3.0. I don't know whether that is the expected version, as I can't find any statement about what is required in the Galaxy tool dependencies for RepeatMasker. Anyway, when a job

Re: [galaxy-dev] Only custom genomes for uploads in data libraries

2013-01-27 Thread Jeremy Goecks
So my quick hack broke this: https://bitbucket.org/galaxy/galaxy-central/commits/0ec7f0b6dec8707b6f4d114468367c9131e61200 To fix this and related issues, I finally took the step of unifying all builds and build information into a single Python data structure:

Re: [galaxy-dev] Only custom genomes for uploads in data libraries

2013-01-25 Thread Jeremy Goecks
So my quick hack broke this: https://bitbucket.org/galaxy/galaxy-central/commits/0ec7f0b6dec8707b6f4d114468367c9131e61200 To fix this and related issues, I finally took the step of unifying all builds and build information into a single Python data structure:

Re: [galaxy-dev] Visualise Galaxy workflows using graphviz

2013-01-21 Thread Jeremy Goecks
please find attached a small patch that add the gen_image() functionality to the export-workflow UI. Thanks for the contribution. I committed a modified version of it: https://bitbucket.org/galaxy/galaxy-central/commits/0e064a23864c846483b35f893cf0a769dedf24f1 Specifically, I (a) used

Re: [galaxy-dev] Redirecting to a URL and breaking the frameset

2013-01-21 Thread Jeremy Goecks
Adding the attribute target=_top to your URL will load the URL in the main browser window rather than a frame. E.g. a href=controller/action target=_topLoad result in top window/a Best, J. On Jan 21, 2013, at 7:24 AM, Paul Boddie wrote: Hello, Is there a convenient recipe for

Re: [galaxy-dev] local install problem with fasta manipulation tools?

2013-01-19 Thread Jeremy Goecks
Two things confuse me about this. First, the tools I want to use are not listed under the list of tools with dependencies.. but I still need to install? what is the difference then between tools with dependencies and all the others? If I understand you correctly, here's the answer: tools with

Re: [galaxy-dev] local install problem with fasta manipulation tools?

2013-01-18 Thread Jeremy Goecks
I hope I have the correct place to ask for assistance. Yes, this list is a great place to ask questions about local Galaxy instances. I've been trying to use a local install of Galaxy. I can upload data and convert formats (FASTq to FASTA) but can't use fasta manipulation tools like width

Re: [galaxy-dev] Integration of STAR Aligner v2.2.0c into Galaxy

2013-01-18 Thread Jeremy Goecks
read in gzipped FASTQ files in the STAR wrapper without writing an external Python script or the like? Thanks, Patrick Von: Jeremy Goecks [mailto:jeremy.goe...@emory.edu] Gesendet: Freitag, 18. Januar 2013 04:37 An: Sorn, Patrick Cc: 'galaxy-dev@lists.bx.psu.edu'; 'do...@cshl.edu

Re: [galaxy-dev] Integration of STAR Aligner v2.2.0c into Galaxy

2013-01-17 Thread Jeremy Goecks
I have a very basic STAR wrapper that works: https://bitbucket.org/jgoecks/jeremys-code/src/fa1930a689b8e2f6b59cc1706e5ba0ed8ad357be/galaxy/tool-wrappers/star.xml?at=default For now, the wrapper does two things: maps RNA-seq reads and identifies chimeric reads/junctions. There are tons of

Re: [galaxy-dev] Anonymous use of workflows and visualization?

2013-01-15 Thread Jeremy Goecks
I'd also been been worried about a similar requirement to ensure reviewers could have anonymous access to software run as a service. In that case even providing dummy accounts to the reviewers might not have been allowed (since the administrator could easily 'spy' on what the reviewers did or

Re: [galaxy-dev] text manipulation Compute tool error

2013-01-14 Thread Jeremy Goecks
Can you reproduce this behavior on our public server? If so, it'll be much easier for us to take a look at the issue. If you can't reproduce on our public server, then providing as many details as possible about the problem will provide the best chance for the list to help you. For instance:

Re: [galaxy-dev] Anonymous use of workflows and visualization?

2013-01-14 Thread Jeremy Goecks
Shared/published visualizations should be able to be used completely anonymously, so that shouldn't be a problem. (Let us know if you find otherwise.) Workflows, however, are tied to user accounts, so using them requires a login for now. Viewing workflows anonymously should be possible,

Re: [galaxy-dev] text manipulation Compute tool error

2013-01-14 Thread Jeremy Goecks
but the job listing in the history is red, and the output is not available for input into other tools. I’ve tried this on other tab-delimited files and it does the same thing. Mark From: Jeremy Goecks [mailto:jeremy.goe...@emory.edu] Sent: Monday, January 14, 2013 12:58 PM

Re: [galaxy-dev] text manipulation Compute tool error

2013-01-14 Thread Jeremy Goecks
with expression c3-c2+1 Kept 100.00% of 96 lines. Thanks Mark From: Jeremy Goecks [mailto:jeremy.goe...@emory.edu] Sent: Monday, January 14, 2013 2:56 PM To: Rose Mark USRE Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] text manipulation Compute tool error If you click

Re: [galaxy-dev] Errors in browser: unknown css properties

2013-01-14 Thread Jeremy Goecks
Trying to fix Trackster on our Galaxy, I noticed that my browser (Firefox) catches a lot of warnings, also on properties of CSS that I thought would be especially for Firefox. Can this cause lagging while using Galaxy? (some of our users experience slow browsing on our Galaxy). No, these

Re: [galaxy-dev] variables not defined

2013-01-07 Thread Jeremy Goecks
These bugs have been fixed in our development repository (galaxy-central) and will be available in the next distribution, which should occur in the coming week. Thanks, J. On Jan 7, 2013, at 6:23 AM, MONJEAUD wrote: Hello all, I am using my own Galaxy instance and I had a problem with

Re: [galaxy-dev] Urgent: Seems like tophat2 does not support --transcriptome-mismatches

2012-12-20 Thread Jeremy Goecks
This thread addresses your issue: http://dev.list.galaxyproject.org/tophat-option-transcriptome-mismatches-not-recognized-td4657568.html In the future, please use the mailing list search before posting your question. You'll save time for both yourself and others.

Re: [galaxy-dev] copying files via galaxy API

2012-12-19 Thread Jeremy Goecks
file_name: /galaxy-central/database/files/000/dataset_733.dat This is the dataset location on the filesystem (perhaps relative . If you script is running on a machine that has access to the filesystem, you should be able to simply copy it. Best,

Re: [galaxy-dev] tophat: option --transcriptome-mismatches not recognized

2012-12-14 Thread Jeremy Goecks
I don't understand why the option is offered in the galaxy UI if it's not available, or is there some kind of bug, or wrong version of tophat2? The parameter has been deprecated in recent versions of Tophat; the wrapper has been updated in -central changeset 4b4ea9952336 to reflect this. J.

Re: [galaxy-dev] Local galaxy

2012-12-13 Thread Jeremy Goecks
Hi I am doing NGS analysis in our local galaxy, I would like to access the output files for further analysis. I do have an account on the server where galaxy is installed, so I would like to access the output files without actually downloading them( aince they are huge). Can you

Re: [galaxy-dev] Tophat for Illumina Error: Could not find Bowtie 2 index files

2012-12-13 Thread Jeremy Goecks
I'm confused. So I installed tophat-2.0.6.Linux_x86_64 which seems to contain tophat and tophat2. tophat2 redirects to tophat, which is tophat version 2.X.X Can't galaxy use that tophat? You can, but you need to use the Tophat2 wrapper, not the Tophat for Illumina wrapper. And, when you

Re: [galaxy-dev] Trying to Run MegaBlast

2012-12-10 Thread Jeremy Goecks
Please see the Megablast section on this page for instructions about getting and adding target databases to Galaxy: http://wiki.galaxyproject.org/Admin/NGS%20Local%20Setup Best, J. On Dec 10, 2012, at 2:59 PM, greg wrote: I have a fresh install on our cluster. I'm trying

Re: [galaxy-dev] Trying to Run MegaBlast

2012-12-10 Thread Jeremy Goecks
Are there any included with Galaxy, or do I need to download a database? You need to download them. Could you guys point me to a good file to test with? Following the directions you sent I got to the point of downloading a file. I tried download

Re: [galaxy-dev] Exporting Trackster visualizations.

2012-12-10 Thread Jeremy Goecks
Unfortunately, no solutions right now; currently, screenshots are best that can be done. One trick we often use for publications/posters is zooming, taking multiple screenshots, and then composing them together; ugly, but pretty effective for showing details. We have some ideas about how to

Re: [galaxy-dev] Data/history/user export and import

2012-12-02 Thread Jeremy Goecks
I was thinking whether I could establish the same from using the DB access directly!? Or would that be too dangerous? In the past I have tried to transfer the user tables from an old instance into a fresh onebut failed due to all sorts of unpredictable errors.. We (the Galaxy team) do

Re: [galaxy-dev] Data/history/user export and import

2012-11-29 Thread Jeremy Goecks
(New feature discussion belong on the -dev list.) This is not possible now but is pretty simple to do in the future: (a) abstract the import/export history functionality into the UsesHistoryMixin; and (b) make import/export available via the API. I've made a Trello card for this:

Re: [galaxy-dev] dynamic column names and R ExpressionSet files

2012-11-28 Thread Jeremy Goecks
if dynamic options allowed a function to be used for retrieving options from a dataset. Best, J. On Nov 28, 2012, at 9:39 AM, S. Horsman wrote: But how does that let me read from a binary file? Bas From: Jeremy Goecks [mailto:jeremy.goe...@emory.edu] Sent: 22 November 2012 15:59

Re: [galaxy-dev] how to get annotation files for rna-seq

2012-11-26 Thread Jeremy Goecks
Thanks Jeremy, Are there any resources linked with Galaxy for viral genome seq annotation ? I'm not aware of any viral genome annotations resources integrated into Galaxy, but NCBI (and others) have virus gene annotations that you upload into Galaxy and use. Best, J.

Re: [galaxy-dev] dynamic column names and R ExpressionSet files

2012-11-22 Thread Jeremy Goecks
Options and filters should work for what you need: http://wiki.galaxyproject.org/Admin/Tools/Tool%20Config%20Syntax#A.3Coption.3E_tag_set http://wiki.galaxyproject.org/Admin/Tools/Tool%20Config%20Syntax#A.3Cfilter.3E_tag_set Best, J. On Nov 22, 2012, at 4:49 AM, S. Horsman wrote: Hi All,

Re: [galaxy-dev] [galaxy-user] help

2012-11-21 Thread Jeremy Goecks
Because your questions are about a local installation of Galaxy, your email is best suited to the galaxy-dev mailing list, so I've moved it there. 1. FastQC not running . gives an error and doesn’t run on groomed or un-groomed FastQ file. The error message shows in the below pic. I

Re: [galaxy-dev] Scatter plot integration in pages

2012-11-15 Thread Jeremy Goecks
Scatterplots will behave just as Trackster does: you'll be able to save scatterplots and embed them in Pages. Looking forward, the Galaxy team is in the process of developing a plug-in framework for visualizations that will allow for arbitrary Web visualizations to work with Galaxy. With this

Re: [galaxy-dev] Trackster error after upgrade

2012-11-13 Thread Jeremy Goecks
One dataset I want to view in Trackster has an error ConverterDependencyException: 'A dependency (bgzip) was in an error state. I've seen this once or twice before but haven't been able to track down the issue yet. Here are two workaround that may help you out: (1) Add the dataset to a

Re: [galaxy-dev] Local data

2012-11-13 Thread Jeremy Goecks
Two ideas: (1) Use data libraries to import all UCSC data files, making sure to link data rather than copy into Galaxy: http://wiki.galaxyproject.org/Admin/Data%20Libraries/Uploading%20Library%20Files (2) Set up a local table browser and modify the ucsc_tablebrowser.xml to point to your local

Re: [galaxy-dev] Tags API

2012-11-12 Thread Jeremy Goecks
Ah, sorry I missed this. From IRC (weeks ago): (03:15:01 PM) jmchilton: Ideally, what would the API uri for assigning a tag to a histories dataset be? POST to api/tags/tag_name/item_class(e.g. HistoryDatasetAssociation)/encoded_id or POST to

Re: [galaxy-dev] Tags API

2012-11-12 Thread Jeremy Goecks
While we're talking about the tags API, I'd also like to see if we can think about the annotations API. Can it be formulated in the same manner as the proposal for the tags api? Yes, annotations and tags share the same structure, so it makes sense that the tag API structure can be used for

Re: [galaxy-dev] Uploading to history via JavaScript

2012-11-12 Thread Jeremy Goecks
What is the error that you're seeing on the server side? I haven't tried this myself, but Brad Chapman suggested this example payload: -- {tool_id: upload1, history_id: identifier of history to use, params: {file_type: vcf, dbkey: hg19, files_0|url_paste:

Re: [galaxy-dev] Tags API

2012-11-10 Thread Jeremy Goecks
Kyle, Tags haven't been implemented in the API yet. This is, of course, planned development for the future. J. On Nov 9, 2012, at 7:56 PM, Kyle Ellrott wrote: Are there any docs or examples about how to add/remove tags from items via the galaxy web-api? Thanks, Kyle Ellrott

Re: [galaxy-dev] Trackster error after upgrade

2012-11-09 Thread Jeremy Goecks
(BITS) http://www.bits.vib.be @bitsatvib On 11/08/2012 04:14 PM, Jeremy Goecks wrote: Try clearing your browser cache and see if that does the trick. If not, please open your JavaScript console and report the errors that you're seeing. Thanks, J. On Nov 8, 2012, at 4:56 AM, Joachim

Re: [galaxy-dev] Trackster error after upgrade

2012-11-08 Thread Jeremy Goecks
Try clearing your browser cache and see if that does the trick. If not, please open your JavaScript console and report the errors that you're seeing. Thanks, J. On Nov 8, 2012, at 4:56 AM, Joachim Jacob wrote: Hi all, After upgrading to the latest galaxy-dist, Trackster does not behave.

Re: [galaxy-dev] can't use bowtie mapper

2012-11-07 Thread Jeremy Goecks
Using my local instance, I was able to run Bowtie2 using a genome in the history. This means the problem is likely with your local instance. This appears to be the problem: Error: could not open Nagenome.fasta Error: Encountered internal Bowtie 2 exception (#1) Command: bowtie2-build -f

Re: [galaxy-dev] JBrowse direct export to Galaxy

2012-11-01 Thread Jeremy Goecks
JBrowse is fully client-side, so there is no jbrowse server we can use. Is there a way to pass data to galaxy directly without the server? This functionality could also be useful for desktop applications. This isn't possible right now, though you could imagine using FTP with the API to

Re: [galaxy-dev] output name of downloaded datasets

2012-10-31 Thread Jeremy Goecks
Downloading data is handled in lib/galaxywebapps/galaxy/controllers/dataset.py, method display(), which in turn calls this line: -- return data.datatype.display_data(trans, data, preview, filename, to_ext, chunk, **kwd) -- Which, in most cases, calls display_data in

Re: [galaxy-dev] Galaxy processing

2012-10-31 Thread Jeremy Goecks
Where do I find info if the installed applications make use of multiple nodes via MPI(etc) which would indicate the benefit of starting up X number of nodes for faster processing? You'll need to look at the individual tool documentation. In general, many tools uses multiple cores, few use

Re: [galaxy-dev] Empty TopHat output

2012-10-31 Thread Jeremy Goecks
Given that this doesn't seem to be happening on our public server or on local instances, my best guess is that the issue is old code. Are you running the most recent dist? J. On Oct 31, 2012, at 7:37 PM, Mohammad Heydarian wrote: We are still getting empty TopHat output files on our Galaxy

Re: [galaxy-dev] Empty TopHat output

2012-10-31 Thread Jeremy Goecks
last week. Thanks! Cheers, Mo Heydarian PhD candidate The Johns Hopkins School of Medicine Department of Biological Chemistry 725 Wolfe Street 414 Hunterian Baltimore, MD 21205 On Wed, Oct 31, 2012 at 8:30 PM, Jeremy Goecks jeremy.goe...@emory.edu wrote: Given

Re: [galaxy-dev] Trackster and gff file with multiple chromosome annotations

2012-10-29 Thread Jeremy Goecks
Whatever the file type I set for the gff file (gff3, gff or gtf), I get the transcript_id error: Traceback (most recent call last): File /home/pgtgal/galaxy-dist/lib/galaxy/datatypes/converters/interval_to_fli.py, line 91, in main() File

Re: [galaxy-dev] Trackster error

2012-10-24 Thread Jeremy Goecks
Can you please share the datasets that are generating this errors? Thanks, J. On Oct 24, 2012, at 4:19 AM, Joachim Jacob wrote: Hi all, I want to view two interval-type datasets in trackster on genome hg19 together with one BAM dataset. The interval datasets give an error (while the

Re: [galaxy-dev] JBrowse direct export to Galaxy

2012-10-24 Thread Jeremy Goecks
Passing it back to Jeremy, there is also a Javascript wrapper around the tools API which might help: https://bitbucket.org/galaxy/galaxy-central/src/tip/static/scripts/mvc/tools.js Yes, the Galaxy team is working on a JavaScript binding to the API, and one of the areas that we've done some

Re: [galaxy-dev] Trackster error

2012-10-24 Thread Jeremy Goecks
of the output of a liftover file (which is in interval format). https://dl.dropbox.com/u/18352887/Galaxy48-%5BSelect_first_on_data_23%5D.interval As you can see, every interval is only one bp long... Thanks Joachim On 10/24/2012 03:27 PM, Jeremy Goecks wrote: Can you please share

Re: [galaxy-dev] JBrowse direct export to Galaxy

2012-10-23 Thread Jeremy Goecks
Erik, The API could be used for this. Specifically, you could do an upload for the user from a URL via the tools API. I know that Brad Chapman (cc'd) has done this successfully recently. Brad, can you share the parameters used to do this? Thanks, J. On Oct 22, 2012, at 3:21 PM, Erik

Re: [galaxy-dev] Trackster and gff file with multiple chromosome annotations

2012-10-23 Thread Jeremy Goecks
Yes, you should be able to use a single GFF for the complete genome. This error stems from the same issue as before, namely that Galaxy is treating your GFF file as GTF. If you think your GFF is well formatted and there is an issue with Galaxy's handling of GFF, please send me your GFF and

Re: [galaxy-dev] Error running cuffmerge

2012-10-20 Thread Jeremy Goecks
Try upgrading your Cufflinks binaries and see if that solves the problem; the wrappers are designed to support versions 1.3+, but I'm not sure if they'll work with older versions. Best, J. On Oct 19, 2012, at 3:22 PM, Iry Witham wrote: Hi Folks, I have run into an issue with cuffmerge. I

Re: [galaxy-dev] Cuff merge and cuffcompare sequence reference

2012-10-19 Thread Jeremy Goecks
Jonas, Cuffmerge and Cuffcompare use the sam_fa_indices.loc for sequence data; this is done for historical reasons. You can see this if you take a look at the wrapper code: galaxy_home/tools/ngs_rna/cuffmerge_wrapper.py galaxy_home/tools/ngs_rna/cuffcompare_wrapper.py Best, J. On Oct 19,

Re: [galaxy-dev] [galaxy-user] Data table named 'bowtie2_indexes' is required by tool but not configured

2012-10-19 Thread Jeremy Goecks
is not working? What might be the problem? On Fri, Oct 19, 2012 at 9:01 AM, Jeremy Goecks jeremy.goe...@emory.edu wrote: You'll need to update the tool_data_table_conf.xml file in your Galaxy home directory. If you haven't made changes to the file, you can copy

Re: [galaxy-dev] Composite datatype output for Cuffdiff

2012-10-12 Thread Jeremy Goecks
Hi Jim, This is nice and is a path forward for the immediate future. That said, a couple extensions to Galaxy to better support composite datatypes would enable cummerbund without the additional tools: (i) extending the composite datatype to include definition of individual outputs in the

Re: [galaxy-dev] Adding metadata to datasets

2012-10-12 Thread Jeremy Goecks
Is there an easy way to add meta data to a dataset without having to resort to coding it into the dataset definition? I am looking for things beyond tags and just a simple key value pair association would be enough... Tags support key-value pairs using this syntax: -- key:value OR

Re: [galaxy-dev] Small bug with search tools in the lastest version of Galaxy

2012-10-11 Thread Jeremy Goecks
My best guess is that these changes are occurring due to the changing corpus of tool titles/descriptions/help text. (Search functionality hasn't been changed in some time.) I've tweaked the search parameters in -central changeset 18ab38e43d8b , specifically removing the minscore requirement,

Re: [galaxy-dev] Velveth wrapper not working with real user jobs

2012-10-10 Thread Jeremy Goecks
Alex, This is definitely an issue, but it is not currently high priority for the Galaxy team. We expect to move the Velvet wrappers to the tool shed in the near future, and from there others can enhance the wrappers as needed. In the meantime, community contributions to the Galaxy code base,

Re: [galaxy-dev] [Patch] Adding interpreter-attribute to the version_command-tag

2012-10-08 Thread Jeremy Goecks
Looks great, committed in -central changeset 02fe49c3d251 Best, J. On Oct 6, 2012, at 6:31 PM, Björn Grüning wrote: Hi all, if we try to wrap a single script-tool, for example bismark [1], it has its own version number and should be included in version_command. I would like to ship

Re: [galaxy-dev] Upload to history via the tool API

2012-10-08 Thread Jeremy Goecks
This looks fantastic Brad and we should be able to integrate it soon. I have a couple questions, though, and I've noted them in the request's comments section. Best, J. On Oct 4, 2012, at 3:44 PM, Brad Chapman wrote: Hi all; I've been thinking about ways to upload into user histories via

Re: [galaxy-dev] Upload to history via the tool API

2012-10-08 Thread Jeremy Goecks
I explained the intent of that code more in the comments and happy to answer any other questions. Everything makes sense; I've accepted the pull request. While you're looking at the API, would you also be willing to consider this pull request:

Re: [galaxy-dev] stack trace from a history problem

2012-10-05 Thread Jeremy Goecks
That's a bug, fixed in cf93e8aa50f9 Thanks, J. On Oct 5, 2012, at 9:34 AM, Langhorst, Brad wrote: On one particular history, I see this. I am at 035583d94b11 I don't know why this happens on this history… I cloned it, then re-ran one step. I hope this stacktrace is helpful:

Re: [galaxy-dev] Instant crash on large history export

2012-10-05 Thread Jeremy Goecks
Alex, We'll need more details if we're going to figure out what the issue is. For example: *are there certain dataset types that lead to a crash? *what does the Galaxy log say (even if it's not interesting)? *does the export job make it into the database and/or onto the cluster? Any other info

Re: [galaxy-dev] Galaxy workflow editor cannot be loaded due to some JS files received 404 not found

2012-10-02 Thread Jeremy Goecks
We are using Sep 7 release of galaxy-dist. Strange enough we have a QA galaxy which is almost identical to the production one except it uses /galaxy_qa as prefix and a different database, but workflow editor works there. The best idea is to update to the most current release (Sept. 20) and

Re: [galaxy-dev] Can't create custom visualization

2012-09-27 Thread Jeremy Goecks
What version of Galaxy are you using? I cannot reproduce the issue using the most recent distribution or galaxy-central. Also, there should be a full stacktrace in your paster.log. Can you share that please? Thanks, J. On Sep 27, 2012, at 11:05 AM, Liisa Koski wrote: Hello, I'm

Re: [galaxy-dev] Can't edit Galaxy Workflow _ElementInterface instance has no attribute 'render'

2012-09-25 Thread Jeremy Goecks
Liisa, My best guess is that this is fixed in the most recent distribution. Can you please update your Galaxy instance to the Sept. 20 distribution and see if that fixes the problem. If not, please let us know. Thanks, J. On Sep 24, 2012, at 11:57 AM, Liisa Koski wrote: Hello, After

Re: [galaxy-dev] where are the tools?

2012-09-25 Thread Jeremy Goecks
Try updating again and see if that fixes the issue. We've been refactoring JavaScript dependencies, which may have caused some temporary problems. In general, if you're going to use/track galaxy-central, you'll need to be both persistent and patient because, as a development repository, things

Re: [galaxy-dev] cufflinks and global_model.txt

2012-09-25 Thread Jeremy Goecks
The Cufflinks wrapper doesn't support v2.0 or greater yet. Please use v1.3.0 for now. We expect to update the Cufflinks wrapper to support v2.0+ in the next couple weeks. Best, J. On Sep 25, 2012, at 5:34 PM, wormbuff wrote: I recently installed Galaxy on Ubuntu 12.04.1 LTS. Bowtie,

Re: [galaxy-dev] Tophat2 Issues

2012-09-18 Thread Jeremy Goecks
The bug manifests itself when running the tool with use Defaults selected results in the following error: cp: cannot stat `/data/galaxy-dev/galaxy-dev/database/job_working_directory/000/281/tophat_out/fusions.out': No such file or directory It seems that somehow, the filter on the

Re: [galaxy-dev] Fix for tophat2 output filter on fusions

2012-09-18 Thread Jeremy Goecks
Fixed in -central changeset 05a172868303 Thanks, J. On Sep 18, 2012, at 4:57 PM, Jim Johnson wrote: The filter on the tophat2 fusions output wasn't being evaluated correctly when settingsType is preSet since the param dict wouldn't then have an entry for 'fusion_search' $ hg diff

<    1   2   3   4   >