Re: [galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2013-04-25 Thread Langhorst, Brad
Hi Dave: Maybe some one knows a better way… I would probably muck around in the database directly if there were a lot of files. One could probably move them all into a history, groom them, then pull the data back into your libraries, deleting the old ones. Brad On Apr 25, 2013, at 4:58 AM, B

Re: [galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2013-04-25 Thread Dave Lin
Hi Brad- In the past, I had always been running the time-consuming fastqgroomer step. However, based on this thread, I now realize this step is sometimes unnecessary. Here is my question. I have a large number of fastq data files that are already in data libaries. They were imported in "fastq" fo

Re: [galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2013-04-25 Thread Langhorst, Brad
Hi dave: The fastq groomer tool will convert your fastq files (of unknown base quality scale) to fastqsanger. Are you sure these files are not already sanger scaled? Modern illumina pipelines produce fastqsanger files. If you do know the scale, just import the files explictly as fastqsanger (n

Re: [galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2013-04-24 Thread Dave Lin
Hi Brad, Galaxy Support- Besides changing them individually, is there a way to modify the settings for a large number files from fastq to fastqsanger in batch? Either via the bioblend or manually via the UI? Thanks, Dave On Fri, Dec 7, 2012 at 3:53 AM, Langhorst, Brad wrote: > You can just c

Re: [galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2012-12-07 Thread Langhorst, Brad
You can just change the format of the data from fastq to fastqsanger if you're sure about the error format (use the pencil, then datatype tab) note: fastqsanger !=fastqcsanger Brad On Dec 7, 2012, at 3:20 AM, 泽 蔡 mailto:caizexi...@yahoo.com.cn>> wrote: Hi Alex Now is an another problem. I now

[galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2012-12-07 Thread 泽 蔡
Hi Alex   Now is an another problem. I now deal with two fastq files, there are Illumina enconding 1.8 and pair-end, so I don't need to groom. But the fact is, I need to use the "filter by quality" and "Fastq interlacer" and without groom thses two tools can not regonize the files. Any idea to s

[galaxy-dev] 回复: 回复: 回复: Speed up the galaxy

2012-12-04 Thread 泽 蔡
Hi, Thanks a lot! 发件人: David Roquis 收件人: caizexi...@yahoo.com.cn; alex.boss...@wur.nl 抄送: galaxy-dev@lists.bx.psu.edu 发送日期: 2012年12月5日, 星期三, 上午 12:31 主题: RE: [galaxy-dev] 回复: 回复: Speed up the galaxy Hi, You can use fastQC to find out what is the qual