---------- Forwarded message ----------
From: מירב . <mer...@gmail.com>
Date: Wed, May 29, 2013 at 8:40 PM
Subject: Re: [galaxy-dev] installing galaxy
To: Ross <ross.laza...@gmail.com>


It worked!! Thank you very mach [?]

Merav


2013/5/29 Ross <ross.laza...@gmail.com>

> Hi, Merav.
> A fully functional Galaxy server relies on a lot of third party software
> and the error message you see is probably because you do not have samtools
> available - the upload process needs it to make an index for your bam file.
> So the solution may be to install the samtools package - eg for ubuntu
> something like:
> sudo apt-get install samtools
>
> After that, if you execute
> samtools
> at a console, you should see something like:
>
> rlazarus@iaas1-int:~$ samtools
>
> Program: samtools (Tools for alignments in the SAM format)
> Version: 0.1.18 (r982:295)
>
> Usage:   samtools <command> [options]
>
> Command: view        SAM<->BAM conversion
>          sort        sort alignment file
>          mpileup     multi-way pileup
>
>
>
> Please take a look at
> http://wiki.galaxyproject.org/Admin/Tools/Tool%20Dependencies
>
>
>
>


On Wed, May 29, 2013 at 8:00 PM, מירב . <mer...@gmail.com> wrote:

> hello,
>
> I am installing  galaxy locally.
>  I tried to upload a  ".bam" file and I got this message:
>
> Traceback (most recent call last):
>   File "/home/user/galaxy_project/tools/data_source/upload.py", line 386, in 
> <module>
>     __main__()
>   File "/home/user/galaxy_project/tools/data_source/upload.py", line 375, in 
> __main__
>     add_file( dataset, registry, json_file, output_path )
>   File "/home/user/galaxy_project/tools/data_source/upload.py", line 314, in 
> add_file
>     if link_data_only == 'copy_files' and 
> datatype.dataset_content_needs_grooming( output_path ):
>   File "/home/user/galaxy_project/lib/galaxy/datatypes/binary.py", line 113, 
> in dataset_content_needs_grooming
>     version = self._get_samtools_version()
>   File "/home/user/galaxy_project/lib/galaxy/datatypes/binary.py", line 97, 
> in _get_samtools_version
>     output = subprocess.Popen( [ 'samtools' ], stderr=subprocess.PIPE, 
> stdout=subprocess.PIPE ).communicate()[1]
>   File "/usr/lib/python2.7/subprocess.py", line 679, in __init__
>     errread, errwrite)
>   File "/usr/lib/python2.7/subprocess.py", line 1259, in _execute_child
>     raise child_exception
> OSError: [Errno 2] No such file or directory
>
>
> This file uploaded  successfully, to the galaxy on the net.
>
> what can it be?
>
>  thanks for your help
>
> merav
>
>
>
>
>
>
>
> ___________________________________________________________
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>



-- 
Ross Lazarus MBBS MPH;
Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444
http://scholar.google.com/citations?hl=en&user=UCUuEM4AAAAJ



-- 
Ross Lazarus MBBS MPH;
Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444
http://scholar.google.com/citations?hl=en&user=UCUuEM4AAAAJ

<<328.png>>

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