Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-13 Thread David Matthews
Hi,

Thanks for the feedback. We are going to try debug=True (it was set to False) 
and get back to you. Our latest traceback is listed below...


Exception happened during processing of request from ('xxx.xxx.xxx.xxx', 59168)
Traceback (most recent call last):
  File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 1053, in process_request_in_thread
self.finish_request(request, client_address)
  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
322, in finish_request
self.RequestHandlerClass(request, client_address, self)
  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
617, in __init__
self.handle()
  File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 432, in handle
BaseHTTPRequestHandler.handle(self)
  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/BaseHTTPServer.py", line 
329, in handle
self.handle_one_request()
  File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 421, in handle_one_request
self.raw_requestline = self.rfile.readline()
  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/socket.py", line 444, in 
readline
data = self._sock.recv(self._rbufsize)
MemoryError




Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk






On 12 Dec 2011, at 21:28, Nate Coraor wrote:

> On Dec 12, 2011, at 5:25 AM, David Matthews wrote:
> 
>> Hi, 
>> 
>> Many thanks for the feedback. Sorry about missing some of the traceback, 
>> here is the full traceback:
>> 
>> Exception happened during processing of request from ('xxx.xxx.xxx.xxx', 
>> 32781)
>> Traceback (most recent call last):
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1053, in process_request_in_thread
>>   self.finish_request(request, client_address)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 322, in finish_request
>>   self.RequestHandlerClass(request, client_address, self)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 617, in __init__
>>   self.handle()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 432, in handle
>>   BaseHTTPRequestHandler.handle(self)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/BaseHTTPServer.py", 
>> line 329, in handle
>>   self.handle_one_request()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 421, in handle_one_request
>>   self.raw_requestline = self.rfile.readline()
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/socket.py", line 444, 
>> in readline
>> Unexpected exception in worker  at 0x2aab12fa9f50>
>> Traceback (most recent call last):
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 863, in worker_thread_callback
>>   runnable()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1037, in 
>>   lambda: self.process_request_in_thread(request, client_address))
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1056, in process_request_in_thread
>>   self.handle_error(request, client_address)
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1044, in handle_error
>>   return super(ThreadPoolMixIn, self).handle_error(request, client_address)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 338, in handle_error
>>   traceback.print_exc() # XXX But this goes to stderr!
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
>> 233, in print_exc
>>   print_exception(etype, value, tb, limit, file)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
>> 125, in print_exception
>>   print_tb(tb, limit, file)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 69, 
>> in print_tb
>>   line = linecache.getline(filename, lineno, f.f_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 14, 
>> in getline
>>   lines = getlines(filename, module_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 40, 
>> in getlines
>>   return updatecache(filename, module_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 
>> 131, in updatecache
>>   line

Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-13 Thread David Matthews
Hi,

We've just re run a Tophat run with debug=true and this is the email I got back 
from our HPC guys:



For the instance that just crashed I ran it interactively i.e. not in daemon 
mode and with debug = True

The only error I'm seeing on the screen is:

./run.sh: line 48:  8705 Segmentation fault  python ./scripts/paster.py 
serve universe_wsgi.ini $@



So we are not much wiser here. As for the question about what path are loading 
- sorry for being a bit slow but what exactly do you mean by that?


Sorry we are eating up so much of your time...

Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk






On 12 Dec 2011, at 21:28, Nate Coraor wrote:

> On Dec 12, 2011, at 5:25 AM, David Matthews wrote:
> 
>> Hi, 
>> 
>> Many thanks for the feedback. Sorry about missing some of the traceback, 
>> here is the full traceback:
>> 
>> Exception happened during processing of request from ('xxx.xxx.xxx.xxx', 
>> 32781)
>> Traceback (most recent call last):
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1053, in process_request_in_thread
>>   self.finish_request(request, client_address)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 322, in finish_request
>>   self.RequestHandlerClass(request, client_address, self)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 617, in __init__
>>   self.handle()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 432, in handle
>>   BaseHTTPRequestHandler.handle(self)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/BaseHTTPServer.py", 
>> line 329, in handle
>>   self.handle_one_request()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 421, in handle_one_request
>>   self.raw_requestline = self.rfile.readline()
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/socket.py", line 444, 
>> in readline
>> Unexpected exception in worker  at 0x2aab12fa9f50>
>> Traceback (most recent call last):
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 863, in worker_thread_callback
>>   runnable()
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1037, in 
>>   lambda: self.process_request_in_thread(request, client_address))
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1056, in process_request_in_thread
>>   self.handle_error(request, client_address)
>> File 
>> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>>  line 1044, in handle_error
>>   return super(ThreadPoolMixIn, self).handle_error(request, client_address)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
>> 338, in handle_error
>>   traceback.print_exc() # XXX But this goes to stderr!
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
>> 233, in print_exc
>>   print_exception(etype, value, tb, limit, file)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
>> 125, in print_exception
>>   print_tb(tb, limit, file)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 69, 
>> in print_tb
>>   line = linecache.getline(filename, lineno, f.f_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 14, 
>> in getline
>>   lines = getlines(filename, module_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 40, 
>> in getlines
>>   return updatecache(filename, module_globals)
>> File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 
>> 131, in updatecache
>>   lines = fp.readlines()
>> MemoryError
> 
> Hi David,
> 
> It looks like something is trying to read way too much data into something 
> else, probably the response.
> 
> 1. is debug = True?
> 2. what path are you loading that yields this result?
> 
> --nate
> 
>> 
>> 
>> We'll look again at the webpage for more clues. As for trying to reproduce 
>> it elsewhere, the data we are using here are all from Galaxy main which has 
>> run the jobs several times without a problem. So we're confident we are 
>> missing something at our end in the set up - presumably to do with memory 
>> usage by TopHat - any thoughts would be most welcome.
>> 
>> Best Wishes,
>> David.
>> 
>> __
>> Dr David A. Matthews
>> 
>> Senior Lecturer in Virology
>> Room E49
>> Department of Cellular and Molecular Medicine

Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-12 Thread Nate Coraor
On Dec 12, 2011, at 5:25 AM, David Matthews wrote:

> Hi, 
> 
> Many thanks for the feedback. Sorry about missing some of the traceback, here 
> is the full traceback:
> 
> Exception happened during processing of request from ('xxx.xxx.xxx.xxx', 
> 32781)
> Traceback (most recent call last):
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 1053, in process_request_in_thread
>self.finish_request(request, client_address)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
> 322, in finish_request
>self.RequestHandlerClass(request, client_address, self)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
> 617, in __init__
>self.handle()
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 432, in handle
>BaseHTTPRequestHandler.handle(self)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/BaseHTTPServer.py", 
> line 329, in handle
>self.handle_one_request()
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 421, in handle_one_request
>self.raw_requestline = self.rfile.readline()
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/socket.py", line 444, 
> in readline
> Unexpected exception in worker  at 0x2aab12fa9f50>
> Traceback (most recent call last):
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 863, in worker_thread_callback
>runnable()
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 1037, in 
>lambda: self.process_request_in_thread(request, client_address))
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 1056, in process_request_in_thread
>self.handle_error(request, client_address)
>  File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
>  line 1044, in handle_error
>return super(ThreadPoolMixIn, self).handle_error(request, client_address)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
> 338, in handle_error
>traceback.print_exc() # XXX But this goes to stderr!
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
> 233, in print_exc
>print_exception(etype, value, tb, limit, file)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 
> 125, in print_exception
>print_tb(tb, limit, file)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 69, 
> in print_tb
>line = linecache.getline(filename, lineno, f.f_globals)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 14, 
> in getline
>lines = getlines(filename, module_globals)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 40, 
> in getlines
>return updatecache(filename, module_globals)
>  File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 
> 131, in updatecache
>lines = fp.readlines()
> MemoryError

Hi David,

It looks like something is trying to read way too much data into something 
else, probably the response.

1. is debug = True?
2. what path are you loading that yields this result?

--nate

> 
> 
> We'll look again at the webpage for more clues. As for trying to reproduce it 
> elsewhere, the data we are using here are all from Galaxy main which has run 
> the jobs several times without a problem. So we're confident we are missing 
> something at our end in the set up - presumably to do with memory usage by 
> TopHat - any thoughts would be most welcome.
> 
> Best Wishes,
> David.
> 
> __
> Dr David A. Matthews
> 
> Senior Lecturer in Virology
> Room E49
> Department of Cellular and Molecular Medicine,
> School of Medical Sciences
> University Walk,
> University of Bristol
> Bristol.
> BS8 1TD
> U.K.
> 
> Tel. +44 117 3312058
> Fax. +44 117 3312091
> 
> d.a.matth...@bristol.ac.uk
> 
> 
> 
> 
> 
> 
> On 10 Dec 2011, at 14:18, Jeremy Goecks wrote:
> 
>> David,
>> 
>> What is the full stack trace that you're seeing? The stack trace is the text 
>> below "Traceback" and identifies the exact location where the problem 
>> occurs. 
>> 
>> Are you following these guidelines:
>> 
>> http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup
>> 
>>  for setting up large genomes in Galaxy?
>> 
>> Also, it would be ideal if you could upload the problematic data--genome + 
>> reads--to our public instance (main.g2.bx.psu.edu) and see if you can 
>> reproduce the problem that you're seeing.
>> 
>> Thanks,
>> J.
>> 
>> On Dec 9, 2011, at 12:20 PM, David Matthews wrote:
>> 
>>> Hi,
>>> 
>>> We seem to have sorted out the problem of TopHat failing to run but now we 
>>> have a new problem. When TopHat runs with a large

Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-12 Thread David Matthews
Hi, 

Many thanks for the feedback. Sorry about missing some of the traceback, here 
is the full traceback:

Exception happened during processing of request from ('xxx.xxx.xxx.xxx', 32781)
Traceback (most recent call last):
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 1053, in process_request_in_thread
   self.finish_request(request, client_address)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
322, in finish_request
   self.RequestHandlerClass(request, client_address, self)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
617, in __init__
   self.handle()
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 432, in handle
   BaseHTTPRequestHandler.handle(self)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/BaseHTTPServer.py", line 
329, in handle
   self.handle_one_request()
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 421, in handle_one_request
   self.raw_requestline = self.rfile.readline()
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/socket.py", line 444, in 
readline
Unexpected exception in worker  at 0x2aab12fa9f50>
Traceback (most recent call last):
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 863, in worker_thread_callback
   runnable()
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 1037, in 
   lambda: self.process_request_in_thread(request, client_address))
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 1056, in process_request_in_thread
   self.handle_error(request, client_address)
 File 
"/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/paste/httpserver.py",
 line 1044, in handle_error
   return super(ThreadPoolMixIn, self).handle_error(request, client_address)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/SocketServer.py", line 
338, in handle_error
   traceback.print_exc() # XXX But this goes to stderr!
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 233, 
in print_exc
   print_exception(etype, value, tb, limit, file)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 125, 
in print_exception
   print_tb(tb, limit, file)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/traceback.py", line 69, 
in print_tb
   line = linecache.getline(filename, lineno, f.f_globals)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 14, 
in getline
   lines = getlines(filename, module_globals)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 40, 
in getlines
   return updatecache(filename, module_globals)
 File "/gpfs/cluster/isys/galaxy/Galaxy/lib/python2.6/linecache.py", line 131, 
in updatecache
   lines = fp.readlines()
MemoryError


We'll look again at the webpage for more clues. As for trying to reproduce it 
elsewhere, the data we are using here are all from Galaxy main which has run 
the jobs several times without a problem. So we're confident we are missing 
something at our end in the set up - presumably to do with memory usage by 
TopHat - any thoughts would be most welcome.

Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk






On 10 Dec 2011, at 14:18, Jeremy Goecks wrote:

> David,
> 
> What is the full stack trace that you're seeing? The stack trace is the text 
> below "Traceback" and identifies the exact location where the problem occurs. 
> 
> Are you following these guidelines:
> 
> http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup
> 
>  for setting up large genomes in Galaxy?
> 
> Also, it would be ideal if you could upload the problematic data--genome + 
> reads--to our public instance (main.g2.bx.psu.edu) and see if you can 
> reproduce the problem that you're seeing.
> 
> Thanks,
> J.
> 
> On Dec 9, 2011, at 12:20 PM, David Matthews wrote:
> 
>> Hi,
>> 
>> We seem to have sorted out the problem of TopHat failing to run but now we 
>> have a new problem. When TopHat runs with a large genome (but not with small 
>> genomes), it finishes the run fine and all the data is there but the web 
>> interface falls over about 8 hours into the run and when we try to access 
>> the web based interface we get a Proxy Error. When we restart it all looks 
>> fine. This is the sort of errors our HPC people find:
>> 
>> Exception happened during processing of request from ('XXX.XXX.XXX.XXX', 
>> 32960)
>> Traceback (most recent call last):
>>   File 
>> "/gpfs/cluster/isys/galaxy/Galaxy

Re: [galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-10 Thread Jeremy Goecks
David,

What is the full stack trace that you're seeing? The stack trace is the text 
below "Traceback" and identifies the exact location where the problem occurs. 

Are you following these guidelines:

http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup

 for setting up large genomes in Galaxy?

Also, it would be ideal if you could upload the problematic data--genome + 
reads--to our public instance (main.g2.bx.psu.edu) and see if you can reproduce 
the problem that you're seeing.

Thanks,
J.

On Dec 9, 2011, at 12:20 PM, David Matthews wrote:

> Hi,
> 
> We seem to have sorted out the problem of TopHat failing to run but now we 
> have a new problem. When TopHat runs with a large genome (but not with small 
> genomes), it finishes the run fine and all the data is there but the web 
> interface falls over about 8 hours into the run and when we try to access the 
> web based interface we get a Proxy Error. When we restart it all looks fine. 
> This is the sort of errors our HPC people find:
> 
> Exception happened during processing of request from ('XXX.XXX.XXX.XXX', 
> 32960)
> Traceback (most recent call last):
>   File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/pa
> ste/httpserver.py", line 1053, in process_request_in_thread
> Unexpected exception in worker  at 0x201caed8>
> Traceback (most recent call last):
>   File 
> "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/pa
> ste/httpserver.py", line 863, in worker_thread_callback
> Unhandled exception in thread started by   read(worker 118, stopped 1127647552)>>
> Traceback (most recent call last):
> 
> 
> Do you have any suggestions for what is happening?
> 
> 
> Best Wishes,
> David.
> 
> __
> Dr David A. Matthews
> 
> Senior Lecturer in Virology
> Room E49
> Department of Cellular and Molecular Medicine,
> School of Medical Sciences
> University Walk,
> University of Bristol
> Bristol.
> BS8 1TD
> U.K.
> 
> Tel. +44 117 3312058
> Fax. +44 117 3312091
> 
> d.a.matth...@bristol.ac.uk
> 
> 
> 
> 
> 
> 
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[galaxy-dev] Galaxy web interface falls over when running TopHat

2011-12-10 Thread David Matthews
Hi,

We seem to have sorted out the problem of TopHat failing to run but now we have 
a new problem. When TopHat runs with a large genome (but not with small 
genomes), it finishes the run fine and all the data is there but the web 
interface falls over about 8 hours into the run and when we try to access the 
web based interface we get a Proxy Error. When we restart it all looks fine. 
This is the sort of errors our HPC people find:

Exception happened during processing of request from ('XXX.XXX.XXX.XXX', 32960)
Traceback (most recent call last):
  File "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/pa
ste/httpserver.py", line 1053, in process_request_in_thread
Unexpected exception in worker  at 0x201caed8>
Traceback (most recent call last):
  File "/gpfs/cluster/isys/galaxy/Galaxy/galaxy-dist/eggs/Paste-1.6-py2.6.egg/pa
ste/httpserver.py", line 863, in worker_thread_callback
Unhandled exception in thread started by >
Traceback (most recent call last):


Do you have any suggestions for what is happening?


Best Wishes,
David.

__
Dr David A. Matthews

Senior Lecturer in Virology
Room E49
Department of Cellular and Molecular Medicine,
School of Medical Sciences
University Walk,
University of Bristol
Bristol.
BS8 1TD
U.K.

Tel. +44 117 3312058
Fax. +44 117 3312091

d.a.matth...@bristol.ac.uk






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