Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-24 Thread Hans-Rudolf Hotz
Hi Moritz I am not a galaxy tool shed expert, but I recommend you start with reading these wiki pages: http://wiki.galaxyproject.org/Tool%20Shed http://wiki.galaxyproject.org/InstallingRepositoriesToGalaxy and then try installing BWA via the tool shed and ask (if any) specific questions (

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-23 Thread Moritz Juchler
1. Thanks, I'll try this out, after you reavealed me which version I should use :) 2. Originally I had this workflow to start with https://main.g2.bx.psu.edu/u/mj--/w/ngs , but I at the sam-to-bam conversion I get the "sequences are not currently available for specified build" error when using "lo

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-22 Thread Moritz Juchler
Hey, now I am having a new problem: *Convert SAM to BAM *Tool execution generated the following error message: [samopen] SAM header is present: 93 sequences. Parse error at line 106: sequence and quality are inconsistent /bin/sh: line 1: 27934 Aborted samtools view -bS "/home/trr/

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-22 Thread Moritz Juchler
Hey, with the "tool file" line I got it working :) Thanks a lot. Could you answer the question regarding Tool Shed: "If I would use Tool Shed, could I skip all these manual steps? Is the installation of BWA with Tool Shed also this complicated or is it more simle?" Best Moritz On 17 July 2013 0

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-22 Thread Peter Cock
On Mon, Jul 22, 2013 at 10:40 AM, Moritz Juchler wrote: > Hey, > > now I am having a new problem: Convert SAM to BAM > Tool execution generated the following error message: > > [samopen] SAM header is present: 93 sequences. > Parse error at line 106: sequence and quality are inconsistent > /bin/sh

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-17 Thread Moritz Juchler
Hey, no thats correct I did not use Tool Shed. If I would use it, could I skip all these manual steps? Is the installation of BWA with Tool Shed also this complicated or is it more simle? How did you not follow my steps :) I wrote down everything clearly, at least thats what I was hoping. And no

Re: [galaxy-dev] BWA Illumina Mapping / BWA Reference Genome

2013-07-17 Thread Hans-Rudolf Hotz
Hi Moritz I am struggling to follow what exactly you have done. As far as I can see, you did not use the toolshed (http://wiki.galaxyproject.org/Tool%20Shed) to install the BWA alinger tool, but did all manually? If so, have you added the following line: to the "tool_conf.xml" file, and