Re: [galaxy-dev] Bowtie2 mm9 index

2013-11-08 Thread Jennifer Jackson

Hi Mary,

The data was restored this morning. Good luck with the new run,

Jen
Galaxy team


On 11/7/13 2:57 PM, Jennifer Jackson wrote:

Hello Mary,

The underlying data is not complete for all mm9 reference genomes for 
the Bowtie2/Tophat2 tools, causing the error. It is a known issue and 
we expect to have it corrected very soon now. We consider this 
important and high priority, our apologies for the confusion it caused.


A list of known data issues since the migration of usegalaxy.org are 
listed in this ticket. You can follow the ticket to find out when this 
genome has been restored: https://trello.com/c/SbizUDQt


Best,

Jen
Galaxy team

On 10/29/13 7:43 AM, Davis, Mary wrote:


Greetings---

I tried to run an alignment using Bowtie2 and got this message-

format: bam, database: mm9

Could not locate a Bowtie index corresponding to basename 
"/galaxy/data/mm9/mm9canon/bowtie2_index/mm9canon" Error: Encountered 
internal Bowtie 2 exception (#1) Command: 
/galaxy/software/linux2.6-x86_64/pkg/bowtie2-2.1.0/bin/bowtie2-align 
--wrapper basic


I imported Illumina fastq data, groomed them, and then did the 
analysis using the built-in index mouse, both full and male, and had 
the same error message.


I'm relatively new to this, and don't see what I missed.

Thanks

Mary E. Davis, Ph.D.

Professor

Department of Physiology & Pharmacology

West Virginia University Health Sciences Center

PO Box 9229

Morgantown, WV  26506-9229



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--
Jennifer Hillman-Jackson
http://galaxyproject.org


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--
Jennifer Hillman-Jackson
http://galaxyproject.org

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Re: [galaxy-dev] Bowtie2 mm9 index

2013-11-07 Thread Jennifer Jackson

Hello Mary,

The underlying data is not complete for all mm9 reference genomes for 
the Bowtie2/Tophat2 tools, causing the error. It is a known issue and we 
expect to have it corrected very soon now. We consider this important 
and high priority, our apologies for the confusion it caused.


A list of known data issues since the migration of usegalaxy.org are 
listed in this ticket. You can follow the ticket to find out when this 
genome has been restored: https://trello.com/c/SbizUDQt


Best,

Jen
Galaxy team

On 10/29/13 7:43 AM, Davis, Mary wrote:


Greetings---

I tried to run an alignment using Bowtie2 and got this message-

format: bam, database: mm9

Could not locate a Bowtie index corresponding to basename 
"/galaxy/data/mm9/mm9canon/bowtie2_index/mm9canon" Error: Encountered 
internal Bowtie 2 exception (#1) Command: 
/galaxy/software/linux2.6-x86_64/pkg/bowtie2-2.1.0/bin/bowtie2-align 
--wrapper basic


I imported Illumina fastq data, groomed them, and then did the 
analysis using the built-in index mouse, both full and male, and had 
the same error message.


I'm relatively new to this, and don't see what I missed.

Thanks

Mary E. Davis, Ph.D.

Professor

Department of Physiology & Pharmacology

West Virginia University Health Sciences Center

PO Box 9229

Morgantown, WV  26506-9229



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--
Jennifer Hillman-Jackson
http://galaxyproject.org

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