> 1. We want to use the NGS:Mapping tool 'Map with Bowtie for Illumina'
> which requires 'bowtie (0.12.7) with bowtie indexes'. Do we simply
> download the bowtie software, place it into
> /Users/administrator/galaxy-dist/tools, restart Galaxy, and the tool
> is available under NGS:Mapping ?

Correct, download the bowtie software, install and verify that it
works. Once it does, you can place the binaries (or a symbolic link)
to it in the tools dependecy directory (not tools). You need to
specify the tools dependency directory in your universe_wsgi.ini file.
You can also, see my response to #3. More information:
http://wiki.galaxyproject.org/Admin/Config/Tool%20Dependencies

> 2. Continuing from question #1, does a type of 'package manager' exist
> for automating the installation of tools and dependencies? Can
> mercurial accomplish this or is this the purpose of the Tool Shed?

Yes and no. Tool Shed is like a package manager, but not. More
information here: http://wiki.galaxyproject.org/Tool%20Shed

You will still need to install the tools, like bowtie, on your own and
not via Galaxy.

> 3. What is the purpose of the Galaxy-specific environment file and how
> is it used?

Going off your question one, you could install bowtie anywhere. I
think on OSX its /Applications/Bowtie2.app/ or some other locations
/opt/bowti2/ etc. Then simply have a reference to using env.sh to
setup the correct environmental paths. In your tools dependency folder
you would have the following setup:

tools_dependecy/bowtie2/1.03/env.sh
tools_dependecy/bowtie2/default --> 1.03/

Note: default is a symbolic link to 1.03

> 4. We have two hard drives: drive1 and drive2. Galaxy is installed on
> drive1 and our data files are on drive2. Will Galaxy know how to find
> the location of our data files on drive2? Also, will Galaxy know how
> to write the result of a job to drive2?

Yes, see universe_wsgi.ini for this. Just update the paths. On OSX it
could be /Volumes/someNameOfDrive/folder/on/drive

> 5. Does Galaxy come configured with an admin account and, if so, how
> is it accessed?

Yes, see universe_wsgi.ini (admin_users). You need to add an email
address of the admin account.

> 6. Does Galaxy use as many hardware resources as possible to execute
> each job (for example, all 12 cores)? If not, can it be configured to
> do so?

You will need to configure your job runners and job workers for this.
http://wiki.galaxyproject.org/Admin/Config/Performance/Cluster



Enjoy!
-Adam

--
Adam Brenner
Computer Science, Undergraduate Student
Donald Bren School of Information and Computer Sciences

Research Computing Support
Office of Information Technology
http://www.oit.uci.edu/rcs/

University of California, Irvine
www.ics.uci.edu/~aebrenne/
aebre...@uci.edu


On Wed, Jun 5, 2013 at 11:22 AM, Nicholas Kline <nx...@case.edu> wrote:
> Hi,
>
> Our lab is in the process of installing a local version of Galaxy on a
> computer with the following specs:
>
> - Mac Pro mid-2012
> - OS X 10.8.3
> - 2 2.4 GHz Intel Xeon Processors
> - 6 cores per processor, 12 cores total
> - 64 GB RAM
>
> This installation is meant to be used by a single user. We followed
> the instructions at http://wiki.galaxyproject.org/Admin/Get%20Galaxy
> and now have a working version of Galaxy available at localhost:8080,
> but we have a few questions before using it:
>
> 1. We want to use the NGS:Mapping tool 'Map with Bowtie for Illumina'
> which requires 'bowtie (0.12.7) with bowtie indexes'. Do we simply
> download the bowtie software, place it into
> /Users/administrator/galaxy-dist/tools, restart Galaxy, and the tool
> is available under NGS:Mapping ?
>
> 2. Continuing from question #1, does a type of 'package manager' exist
> for automating the installation of tools and dependencies? Can
> mercurial accomplish this or is this the purpose of the Tool Shed?
>
> 3. What is the purpose of the Galaxy-specific environment file and how
> is it used?
>
> 4. We have two hard drives: drive1 and drive2. Galaxy is installed on
> drive1 and our data files are on drive2. Will Galaxy know how to find
> the location of our data files on drive2? Also, will Galaxy know how
> to write the result of a job to drive2?
>
> 5. Does Galaxy come configured with an admin account and, if so, how
> is it accessed?
>
> 6. Does Galaxy use as many hardware resources as possible to execute
> each job (for example, all 12 cores)? If not, can it be configured to
> do so?
>
> Thank you for your help!
> ___________________________________________________________
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