Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Dear folks, Why could not I find BDGP 5.74 in the drop-down menu of SnpEff? If I want to use this version, what should I do or what other steps do I need to install it? Thank you so much! From: Wang, Xiaofei Sent: Monday, April 14, 2014 9:50 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: RE: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Dear there, Could someone tell me why there is no Drosophila melanogaster : BDGP 5.74 in the drop-down menu of SnpEff for local Galaxy? In fact, I could find it (BDGP 5.74) in SnpEff Databases and also download it from SnpEff Download (Data manager: Admin Manage local data (beta)). But, I still can not find it in the menu of SnpEff after downloading and installing it by the two-steps as below. Thanks a lot! Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Friday, April 11, 2014 5:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors I am sorry to bother again about this problem. I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the Genome drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.) Thanks Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42 Thank you so much! Best, Xiaofei _ From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is: java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42 at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem? May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times): Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Dear there, Could someone tell me why there is no Drosophila melanogaster : BDGP 5.74 in the drop-down menu of SnpEff for local Galaxy? In fact, I could find it (BDGP 5.74) in SnpEff Databases and also download it from SnpEff Download (Data manager: Admin Manage local data (beta)). But, I still can not find it in the menu of SnpEff after downloading and installing it by the two-steps as below. Thanks a lot! Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Friday, April 11, 2014 5:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors I am sorry to bother again about this problem. I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the Genome drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.) Thanks Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42 Thank you so much! Best, Xiaofei _ From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is: java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42 at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem? May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times): Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. It's a little bit complicated. If you click on the SnpEff Download under Data Manager a progress ist started that will fetch a list
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
I am sorry to bother again about this problem. I would like to use Drosophila melanogaster : BDGP5.74. I did the two steps you suggested to me to install the annotation. I also did from command line. But, there is no the option of BDGP5.74 from the Genome drop-down menu in SnpEff. The version of SnpEff is version 1.0 and I used SnpEfff in CloudMap workflow. Do you know why and how to figure it out? (In fact, it works fine for BDGP5.66.) Thanks Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42 Thank you so much! Best, Xiaofei _ From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is: java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42 at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem? May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times): Data Manager: snpeff_genomedb value name path dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. It's a little bit complicated. If you click on the SnpEff Download under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool SnpEff Databases there you can choose which genome annotation you want to install. To sum it up. 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin Data Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why? Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. It's a little bit complicated. If you click on the SnpEff Download under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool SnpEff Databases there you can choose which genome annotation you want to install. To sum it up. 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin Data Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why? Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel? Cheers, Bjoern When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time? Thank you so much! Best, Xiaofei From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin Data Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty. After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. Hope that will help! Bjoern Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel? I really appreciate for your help, and thank you so much for your patience! Best, Xiaofei Data Manager Choose your data managing option from below. * Access data managers - get data, build indexes, etc * SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database * BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by manager * SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database * BWA indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by Tool Data Table
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is: java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42 at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem? May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times): Data Manager: snpeff_genomedb value namepath dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. It's a little bit complicated. If you click on the SnpEff Download under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool SnpEff Databases there you can choose which genome annotation you want to install. To sum it up. 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin Data Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why? Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel? Cheers, Bjoern When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time? Thank you so much! Best, Xiaofei From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin Data Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty. After SnpEff Databases Execute you still need to pick genome
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Dear there, I am still stuck there for FASTQ Summary Statistics. Could you give me some clues to fix it? Thanks a lot! Best, Xiaofei From: Wang, Xiaofei Sent: Wednesday, April 02, 2014 11:21 AM To: Daniel Blankenberg Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: RE: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, yes, here it is: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in module if __name__ == __main__: main() File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 32, in main base_counts = aggregator.get_base_counts_for_column( i ) File /Users/xiaofeiwang/softwares/galaxy-dist/lib/galaxy_utils/sequence/fastq.py, line 397, in get_base_counts_for_column return self.nuc_index_base[ column ] IndexError: list index out of range From: Daniel Blankenberg [d...@bx.psu.edu] Sent: Wednesday, April 02, 2014 11:14 AM To: Wang, Xiaofei Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, To get the complete error message, can you click on the bug icon (left-most icon at the bottom of the expanded history item) of the error’d dataset and copy and paste from the page that loads in the middle pane? Thanks for using Galaxy, Dan On Apr 2, 2014, at 12:04 PM, Wang, Xiaofei xfw...@ku.edu wrote: Can you give us the complete error message. The important part is missing. Here is the whole thing for the error: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/xiaofe I know it seems weird, but it is the complete message. May I ask a question about this? Is it required rpy for FASTQ Summary Statistics or CloundMap? Thanks a lot! From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei, Can you provide some sample data that is causing the issue for you? Thanks, Dan On Apr 3, 2014, at 11:46 AM, Wang, Xiaofei xfw...@ku.edu wrote: Dear there, I am still stuck there for FASTQ Summary Statistics. Could you give me some clues to fix it? Thanks a lot! Best, Xiaofei From: Wang, Xiaofei Sent: Wednesday, April 02, 2014 11:21 AM To: Daniel Blankenberg Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: RE: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, yes, here it is: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in module if __name__ == __main__: main() File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 32, in main base_counts = aggregator.get_base_counts_for_column( i ) File /Users/xiaofeiwang/softwares/galaxy-dist/lib/galaxy_utils/sequence/fastq.py, line 397, in get_base_counts_for_column return self.nuc_index_base[ column ] IndexError: list index out of range From: Daniel Blankenberg [d...@bx.psu.edu] Sent: Wednesday, April 02, 2014 11:14 AM To: Wang, Xiaofei Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, To get the complete error message, can you click on the bug icon (left-most icon at the bottom of the expanded history item) of the error’d dataset and copy and paste from the page that loads in the middle pane? Thanks for using Galaxy, Dan On Apr 2, 2014, at 12:04 PM, Wang, Xiaofei xfw...@ku.edu wrote: Can you give us the complete error message. The important part is missing. Here is the whole thing for the error: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/xiaofe I know it seems weird, but it is the complete message. May I ask a question about this? Is it required rpy for FASTQ Summary Statistics or CloundMap? Thanks a lot! From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download the database by command again: java -jar snpEff.jar download dm5.42 Thank you so much! Best, Xiaofei _ From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Yes, I need to wait a few minutes to get response. But, after these two steps, I got an error again when I run the snpEff, here it is: java.lang.RuntimeException: ERROR: Cannot read file '/Users/pablocingolani/snpEff/./data/dm5.42/snpEffectPredictor.bin'. You can try to download the database by running the following command: java -jar snpEff.jar download dm5.42 at ca.mcgill.mcb.pcingola.snpEffect.SnpEffectPredictor.load(SnpEffectPredictor.java:58) at ca.mcgill.mcb.pcingola.snpEffect.Config.loadSnpEffectPredictor(Config.java:377) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:1006) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) I tried to download the database by command line: java -jar snpEff.jar download dm5.42, but still got this error. How should I figure out this problem? May I ask one more question about this? After 1 and 2 steps, what is it going to be shown in Data Manager? I mean in snpeff annotation, the third part of Data Manager (View managed data by Tool Data Table). When I click on snpeff annotations on View managed data by Tool Data Table, there is nothing there. Is it correct? Actually, when I clicked on snpeff genomedb, it showed like this (I installed the genome several times): Data Manager: snpeff_genomedb value namepath dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data dm5.42 dm5.42 : Drosophila_melanogaster : dm5.42 /Users/xiaofeiwang/softwares/galaxy-dist/tool-data/snpEff/data From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. It's a little bit complicated. If you click on the SnpEff Download under Data Manager a progress ist started that will fetch a list of available genomes that in a next step is shown under the tool SnpEff Databases there you can choose which genome annotation you want to install. To sum it up. 1. click SnpEff Download 2. choose one genome from SnpEff Databases and install it Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin Data Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why? Then there is something missing from your reinstallation. The data manager is a dependency of the snpeff package and should be installed as well. Can you check if you need to reinstall the dependency manually unter the snpeff tool in your admin panel? Cheers, Bjoern When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time? Thank you so much! Best, Xiaofei From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Can you give us the complete error message. The important part is missing. Here is the whole thing for the error: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/xiaofe I know it seems weird, but it is the complete message. May I ask a question about this? Is it required rpy for FASTQ Summary Statistics or CloundMap? Thanks a lot! From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei, To get the complete error message, can you click on the bug icon (left-most icon at the bottom of the expanded history item) of the error’d dataset and copy and paste from the page that loads in the middle pane? Thanks for using Galaxy, Dan On Apr 2, 2014, at 12:04 PM, Wang, Xiaofei xfw...@ku.edu wrote: Can you give us the complete error message. The important part is missing. Here is the whole thing for the error: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/xiaofe I know it seems weird, but it is the complete message. May I ask a question about this? Is it required rpy for FASTQ Summary Statistics or CloundMap? Thanks a lot! From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Oh, yes, here it is: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in module if __name__ == __main__: main() File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 32, in main base_counts = aggregator.get_base_counts_for_column( i ) File /Users/xiaofeiwang/softwares/galaxy-dist/lib/galaxy_utils/sequence/fastq.py, line 397, in get_base_counts_for_column return self.nuc_index_base[ column ] IndexError: list index out of range From: Daniel Blankenberg [d...@bx.psu.edu] Sent: Wednesday, April 02, 2014 11:14 AM To: Wang, Xiaofei Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, To get the complete error message, can you click on the bug icon (left-most icon at the bottom of the expanded history item) of the error’d dataset and copy and paste from the page that loads in the middle pane? Thanks for using Galaxy, Dan On Apr 2, 2014, at 12:04 PM, Wang, Xiaofei xfw...@ku.edu wrote: Can you give us the complete error message. The important part is missing. Here is the whole thing for the error: Traceback (most recent call last): File /Users/xiaofeiwang/softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/xiaofe I know it seems weird, but it is the complete message. May I ask a question about this? Is it required rpy for FASTQ Summary Statistics or CloundMap? Thanks a lot! From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Yes, it is figured, when I changed the java version to 1.7. Thank you so much! But, I got another error for snpEff. java.lang.RuntimeException: Property: 'dm5.30.genome' not found at ca.mcgill.mcb.pcingola.interval.Genome.init(Genome.java:92) at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:96) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) param name=genomeVersion type=select label=Genome option value=hg37Human (hg37)option option value=mm37.61Mouse (mm37.61)option param From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi, that means that the snpeff annotation is missing. You can install it with the snpeff datamanager under your Admin panel. Have a look at the wiki page about data-manager and the snpeff wrapper documentation. You do not need to edit xml files. If so that is an bug in the wrapper and we should fix it :) Cheers, Bjoern Am 02.04.2014 19:34, schrieb Wang, Xiaofei: When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Yes, it is figured, when I changed the java version to 1.7. Thank you so much! But, I got another error for snpEff. java.lang.RuntimeException: Property: 'dm5.30.genome' not found at ca.mcgill.mcb.pcingola.interval.Genome.init(Genome.java:92) at ca.mcgill.mcb.pcingola.snpEffect.Config.readGenomeConfig(Config.java:481) at ca.mcgill.mcb.pcingola.snpEffect.Config.readConfig(Config.java:444) at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:345) at ca.mcgill.mcb.pcingola.snpEffect.Config.init(Config.java:96) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.readConfig(SnpEff.java:245) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:981) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:963) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:352) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:320) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:83) What does this mean? I found the similar error on this thead http://dev.list.galaxyproject.org/snpEff-on-Galaxy-td4659332.html, but there is no reply. Does this mean I need to add genome to Galaxy? Following this webpage: http://snpeff.sourceforge.net/SnpEff_manual.html? If yes, could you give me an example how to edit the snpEffect.xml file in dm (Drosophila) case as below? (In fact, on the drap-down menu of Genome in snpEff, there is different options for genome. Why the error shows '*.genome' not found? ) param name=genomeVersion type=select label=Genome option value=hg37Human (hg37)option option value=mm37.61Mouse (mm37.61)option param From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j Which Java version do you use. Please make sure you are running Java 1.7. Also, when I used the FASTQ Summary Statistics, I got an error like this: Traceback (most recent call last): File /Users/./softwares/shed_tools/toolshed.g2.bx.psu.edu/repos/devteam/fastq_stats/9b7b4e0ca9db/fastq_stats/fastq_stats.py, line 48, in if __name__ == __main__: main() File /Users/... Can you give us the complete error message. The important part is missing. Ciao, Bjoern I tried to google and solve them by myself. But I really do not know how to do that. Could anybody give me some clue? Thanks a lot for your help! ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/ ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/mailinglists/
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin Data Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty. Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel? I really appreciate for your help, and thank you so much for your patience! Best, Xiaofei Data Manager Choose your data managing option from below. * Access data managers - get data, build indexes, etc * SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database * BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by manager * SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database * BWA indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by Tool Data Table * snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases * snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb * snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb * snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations * bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes * bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color * all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta * * * * * From: Björn Grüning [bjoern.gruen...@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: *When I used SnpEff in local galaxy instance, I got an error: *Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at java.lang.ClassLoader.defineClassCond(ClassLoader.j * * * * *Which Java version do you use. Please make sure you are running Java 1.7. * * * * *Yes, it is figured, when I changed
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin Data Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty. After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. Hope that will help! Bjoern Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel? I really appreciate for your help, and thank you so much for your patience! Best, Xiaofei Data Manager Choose your data managing option from below. * Access data managers - get data, build indexes, etc * SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database * BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by manager * SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database * BWA indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by Tool Data Table * snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases * snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb * snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb * snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations * bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes * bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color * all_fastahttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=all_fasta * * * * * From: Björn Grüning [bjoern.gruen...@gmail.com] * Sent: Wednesday, April 02, 2014 12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel. Have a look at the * wiki page about data-manager and the snpeff wrapper documentation. * You do not need to edit xml files. If so that is an bug in the wrapper * and we should fix it :) * * Cheers, * Bjoern * * Am 02.04.2014 19:34, schrieb Wang, Xiaofei: *When I used SnpEff in local galaxy instance, I got an error: *Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1
Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors
After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. You mean SnpEff Download under Data Manager? But, there is no response when I click on it. Unfortunately, I tried to uninstall the snpEff tool shed and re-install it again to see if I can get response. But, when I go to Admin Data Manage local data (beta) this time, there is no snpEff data manager and only the BWA and Fetching genome (I installed them by myself) are left there. Do you know why? When I search the tool sheds like BWA data manager, I did not find there is snpEff data manager. Do you know where could I find it? Why the snpEff data manager has been there last time? Thank you so much! Best, Xiaofei From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did is: Admin Data Manage local data (beta), then the message is shown as below. (For the BWA and Fetching genome, I installed them from tool_sheds by myself. For the SnpEff staff, it has been there when I checked the data manager.) Next, I clicked on SnpEff Databases Execute. But, when I checked the snpeff annotations in View managed data by Tool Data Table part, it is still empty. After SnpEff Databases Execute you still need to pick genome of interest and run SnpEff Download. SnpEff Databases just downloads a list of available genomes. Hope that will help! Bjoern Could you give me more details about how to install the annotation with the snpeff data manager under the Admin panel? I really appreciate for your help, and thank you so much for your patience! Best, Xiaofei Data Manager Choose your data managing option from below. * Access data managers - get data, build indexes, etc * SnpEff Databaseshttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_databases%2F3.4 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager_snpeff_download%2F3.4 - Download a new database * BWA indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_index_builder_data_manager%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fbwa_color_space_index_builder_data_manager%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/tool_runner?tool_id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager_fetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by manager * SnpEff Databaseshttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_databases%2F0.0.1 - Read the list of available snpEff databases * SnpEff Downloadhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fiuc%2Fsnpeff%2Fdata_manager%2Fdata_manager_snpeff_download%2F0.0.1 - Download a new database * BWA indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_index_builder%2F0.0.1 - builder * BWA Color indexhttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_bwa_index_builder%2Fdata_manager%2Fbwa_color_space_index_builder%2F0.0.1 - builder * Reference Genomehttp://127.0.0.1:8080/data_manager/manage_data_manager?id=toolshed.g2.bx.psu.edu%2Frepos%2Fdevteam%2Fdata_manager_fetch_genome_all_fasta%2Fdata_manager%2Ffetch_genome_all_fasta%2F0.0.1 - fetching * View managed data by Tool Data Table * snpeff_databaseshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_databases * snpeff_genomedbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_genomedb * snpeff_regulationdbhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_regulationdb * snpeff_annotationshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=snpeff_annotations * bwa_indexeshttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes * bwa_indexes_colorhttp://127.0.0.1:8080/data_manager/manage_data_table?table_name=bwa_indexes_color
