Re: [galaxy-user] Question about tool

2013-03-27 Thread Jennifer Jackson
Hi Sandra, Galaxy can certainly be used to correlate a set of unknown genomic coordinates with another annotated set of genomic corrdinates, such as those available from UCSC, Biomart, etc. The tool set to look at is Operate on genomic intervals and an example of how these tools are used,

Re: [galaxy-user] glitch in .wig visualization in trackster?

2013-03-27 Thread James Taylor
Hi Michael, sorry it took a while but we've figured out the root cause of this issue and will be deploying a fix shortly. Thanks! -- James Taylor, Assistant Professor, Biology/CS, Emory University On Fri, Mar 22, 2013 at 4:20 PM, Michael Axtell mj...@psu.edu wrote: Hi everyone. I'm having an

[galaxy-user] is there size limit of dataset for running Tophat?

2013-03-27 Thread Du, Jianguang
Hi All, Is there a size limit of dataset for running Tophat at Galaxy? If there is, how many reads is the limit? Thanks. Jianguang ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the

[galaxy-user] Internal Server Error and slow to refresh History

2013-03-27 Thread Gene Genome
Hi All, When I tried to download the filtered datasets from my History at Galaxy to my computer, I always got this error information: Internal Server Error Galaxy was unable to sucessfully complete your request An error occurred. This may be an intermittent problem due to load or other

Re: [galaxy-user] glitch in .wig visualization in trackster?

2013-03-27 Thread Michael Axtell
James, great! Glad you isolated the bug and will fix. It will come in handy for us (and maybe others). Mike On Wed, Mar 27, 2013 at 2:07 PM, James Taylor ja...@jamestaylor.org wrote: Hi Michael, sorry it took a while but we've figured out the root cause of this issue and will be deploying a

Re: [galaxy-user] is there size limit of dataset for running Tophat?

2013-03-27 Thread Jennifer Jackson
Hi Jianguang, The limit for Tophat will most likely not be the number of reads, but the total processing time when using the public Galaxy instance. Currently, a job has 72 hours to complete, assuming that there is not a memory problem before that time limit is reached. But, there are some

Re: [galaxy-user] Depth command in SAM tools

2013-03-27 Thread Jennifer Jackson
Hello Els, Have you seen the tool BEDTools - Create a BedGraph of genome coverage? This would give you the coverage numbers, then you could perform statistics on those numbers. You could also Convert from BAM to BED (there is an option to split for spliced alignments) and if you had a bed