[galaxy-user] illogical results in Tophat2 analysis

2014-04-07 Thread Si Naftaly
Hello, I used a Tophat 2 analysis and got 99.2% discordant in the summary, while in fact I checkedthe distance between the reads and got 170 with std of 35 (this is the data i filled in the Tophat2). Why did the Tophat2 test found 99.2% discordant when it should have been

[galaxy-user] setting dbkey in a workflow

2014-04-07 Thread William Holtz
I'm working on making my first workflow in Galaxy, using a local server. A high level overview of the workflow is: 1) take a fasta file as input with dbkey set to organism1 2) blast input fasta file against organism2 database 3) manipulate the blast output into bed format 4) extract genomic DNA

Re: [galaxy-user] illogical results in Tophat2 analysis

2014-04-07 Thread Jennifer Jackson
Hi Si, Would you like to share your history with me? Note which dataset has the run with the issue and make sure that all inputs, including upload and Fastq Groomer (if done), are present and undeleted. How to share: https://wiki.galaxyproject.org/Support#Shared_and_Published_data Thanks!

Re: [galaxy-user] setting dbkey in a workflow

2014-04-07 Thread Jennifer Jackson
Hi Will, Agreed, this would make a useful Edit Step Actions function. More are already planned and I asked our dev team to specifically consider this as an enhancement in this Trello ticket: https://trello.com/c/Gy0ZZpCc If there if another work-around and I've missed it (development is a