[galaxy-user] ChIP-seq data analysis question

Hello, My name is Christopher Terranova and am a M.S student at the University of Buffalo SUNY.I have been attempting to analyze my MACS data using Galaxy, already have my custom peaks on the UCSC Genome browser and have some specific questions. I am attempting to show how my peaks (and peak cen

Re: [galaxy-user] Chip-seq data

Giuseppe, Your ChipSeq data is already in fastq format. It appears to have Illunima quality scores, so you'll need to use the NGS:QC and manipulation > FASTQ Groomer tool, using 'Illumina 1.3+' as input and 'Sanger' quality format as output. As to using MACS, I've never used it before but you shoul

[galaxy-user] Chip-seq data

Hi, I have illumina ChipSeq data in txt format with this structure: @HWI-EAS225:8:1:1:58#0/1 NAGAGTGCCCGGGTTCAGTTCTCAGCACCCATGTGG +HWI-EAS225:8:1:1:58#0/1 DMSSUSSTTTUTSRQRTTTSSSUS @HWI-EAS225:8:1:1:1803#0/1 NCCATGGGAAGAGCTGGGCAGGCGGGCCGAGCGAAG +HWI-EAS225:8:1:1:1803#0/1 DLSTTSKOUTRRTTS