Re: [galaxy-user] Identification of replicate outlier

c) if you can create an appropriate input matrix (read counts by exon or other contig for each sample eg), the Principal Component Analysis tool might be helpful (library size normalization is one devil that lies in the detail and it's not quite the same as MDS - see below) I like starting

Re: [galaxy-user] Identification of replicate outlier

Hi Ross, Thanks for the suggestions. I'm aware that this is not really a Galaxy-specific question, and I've been browsing through SeqAnswers and found a couple of suggestions using edgeR or DESeq, but nothing for Tuxedo suite. However, I have no experience with either of these tools, so I was

Re: [galaxy-user] Identification of replicate outlier

Hi Dave, This is an interesting and non-trivial question that extends well beyond Galaxy - and there's no simple solution AFAIK Defining an 'outlier' tends to boil down to subjective judgement in most real cases I've seen. EG: see