Re: [galaxy-user] visualization through Trackster on local Galaxy installation

2014-01-31 Thread Yupu Liang
Using full path in the ini file fix the problem!

Thanks,
Yupu
On Jan 30, 2014, at 10:21, Jennifer Jackson j...@bx.psu.edu wrote:

 Hi Yupu,
 
 I'm sorry, I mixed up the processing for custom builds with the processing 
 for installed builds in my initial reply. A .2bit  .len file are appropriate 
 for what you are doing - and the wiki instructions are accurate.
 
 Still, I double checked with Jeremy, the scientist that developed the tool, 
 about your issue. He found that the full path to the .len file needs to be 
 specified right now in order for installed builds to function correctly.
 
 This deviates from the wiki instructions, but we consider this a bug and plan 
 to correct it in the next release.
 
 Please try using the full path and let us know how that works out,
 
 Jen
 Galaxy team
 
 On 1/29/14 11:54 AM, Yupu Liang wrote:
 Dear Jennifer:
 
 Hi, I did put 2bit file as suggested by 
 https://wiki.galaxyproject.org/Visualization%20Setup.
 
 Should I get rid of the len file?  and I assume I will also need to take off 
 the len_file_path out of the universe_wsgi.ini file.
 
 In term of using .fasta file. I have them and I am not clear where I should 
 put them for visualization purpose and also what adjustment i need to make 
 in the ini file. Any help would be appreciated and I am really excited about 
 doing all the visualization within Galaxy. I think the biologists would 
 really love these features.
 
 Thanks a lot,
 Yupu
 
 
 
 
 On Jan 29, 2014, at 14:21, Jennifer Jackson j...@bx.psu.edu wrote:
 
 Hello Yupu,
 
 Using a .len file is problematic at this time. In the upcoming release this 
 will be corrected. Using a .fasta file is the solution. In many cases, 
 using a .fasta file can be preferred as it will include the reference 
 genome sequence in the visualization, but the choice is yours, once the 
 correction is released to the distribution.
 
 More about this is in a recent post from Jeremy:
 http://lists.bx.psu.edu/pipermail/galaxy-user/2014-January/007106.html
 
 Our apologies for the confusion this caused. The advice from Hans-Rudolf is 
 under normal circumstances the best first-pass route for troubleshooting, 
 and you will want to restart after using the fasta method as well.
 
 Best,
 
 Jen
 Galaxy team
 
 On 1/28/14 11:12 AM, Yupu Liang wrote:
 Hi,
 
 I am trying to set up the visualization functionality on our local Galaxy 
 installation. I followed the instructions on  
 https://wiki.galaxyproject.org/Visualization%20Setup
 
 And here is the list of len files:
 ls -l tool-data/shared/ucsc/chrom/
 total 200
 -rw-rw-r-- 1 galaxy galaxy10 Jan 27 15:13 centromeres1.len
 -rw-rw-r-- 1 galaxy galaxy   821 Jan 27 15:13 hg15.len
 -rw-rw-r-- 1 galaxy galaxy   713 Jan 27 15:13 hg16.len
 -rw-rw-r-- 1 galaxy galaxy   792 Jan 27 15:13 hg17.len
 -rw-rw-r-- 1 galaxy galaxy   855 Jan 27 15:13 hg18.len
 -rw-rw-r-- 1 galaxy galaxy   202 Jan 27 15:13 hg19Haps.len
 -rwxr-xr-x 1 galaxy galaxy  1971 Jan 27 15:13 hg19.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 hg19LggInv.len
 -rw-rw-r-- 1 galaxy galaxy  4516 Jan 27 15:13 hg19Patch10.len
 -rw-rw-r-- 1 galaxy galaxy  1983 Jan 27 15:13 hg19Patch2.len
 -rw-rw-r-- 1 galaxy galaxy  2844 Jan 27 15:13 hg19Patch5.len
 -rw-rw-r-- 1 galaxy galaxy  3879 Jan 27 15:13 hg19Patch9.len
 -rw-rw-r-- 1 galaxy galaxy 11672 Jan 27 15:13 hg38.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 hlaRef1.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 hlaRef2.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 hlaRef3.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 repBase0.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 repBase1.len
 -rw-rw-r-- 1 galaxy galaxy 12697 Jan 27 15:13 repeats2.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 simHuman.len
 -rw-rw-r-- 1 galaxy galaxy67 Jan 27 15:13 simHumanMammal.len
 -rw-rw-r-- 1 galaxy galaxy   362 Jan 27 15:13 venter1.len
 
 
 When I test the visualization through Trackster. I got the following error 
 after seeing a message saying preparing the data, this can take a 
 while……:
 
 Error: The requested genome file (tool-data/shared/ucsc/chrom/hg18.len) 
 could not be opened. Exiting!
 sort: write failed: standard output: Broken pipe
 sort: write error
 Couldn't open tool-data/shared/ucsc/chrom/hg18.len , No such file or 
 directory.
 
 
 It seems something else failed in the middle of visualization as I do have 
 the hg18.len under the default path with the global reading permission……..
 
 Does anybody have similar experience? Is there is way for me to debug?
 
 Best,
 Yupu
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[galaxy-user] problem with scientific notation sorting and filtering in galaxy

2014-01-31 Thread Douglas Cavener
I have found that numbers in scientific notation (e.g 9.8E-9) are incorrectly 
sorted or filtered by Galaxy. Looks like the negative exponent is ignored and 
so if you sorting something like P values (see example below) then you find 
these ordered above P=1 instead at the other end of the list where P is near 
zero. In the example below a KEGG list is ordered on column 5 in asending order 
by galaxy. If you scroll to the bottom of the list, however, you will see the 
smallest numbers all of which are in scientific notation. I should note that I 
can can copy this into excel and excel will properly sort scientific notation. 
So this seems to be a specific problem with Galaxy. I found this problem with 
both filtering and sorting functions of Galaxy. 
Doug Cavener

41  0.5326  1.0 0.025   cfa04146=Peroxisome
39  0.5325  1.0 0.038   cfa04640=Hematopoietic cell 
lineage
29  0.5913  1.0 0.044   cfa00980=Metabolism of 
xenobiotics by cytochrome P450
22  0.6310  1.0 0.16cfa00140=Steroid hormone 
biosynthesis
28  0.5619  1.0 0.27cfa05204=Chemical carcinogenesis
11  0.853   1.0 0.33cfa00511=Other glycan 
degradation
25  0.5716  1.0 0.52cfa00600=Sphingolipid metabolism
9   0.902   1.0 0.74cfa00740=Riboflavin metabolism
23  0.5814  1.0 0.79cfa00380=Tryptophan metabolism
15  0.686   1.0 0.96cfa04977=Vitamin digestion and 
absorption
22  0.5617  1.0 0.00017 cfa00071=Fatty acid metabolism
22  0.5617  1.0 0.00017 cfa05150=Staphylococcus aureus infection
26  0.5327  1.0 0.00017 cfa00982=Drug metabolism - cytochrome 
P450
49  0.4442  1.0 0.00018 cfa04142=Lysosome
56  0.4247  1.0 0.00024 cfa04630=Jak-STAT signaling pathway
34  0.4835  1.0 0.00026 cfa03320=PPAR signaling pathway
13  0.685   1.0 0.00027 cfa00531=Glycosaminoglycan degradation
20  0.5621  1.0 0.00043 cfa04975=Fat digestion and absorption
22  0.5424  1.0 0.00043 cfa00830=Retinol metabolism
19  0.5620  1.0 0.00053 cfa00565=Ether lipid metabolism
14  0.6111  1.0 0.00092 cfa00591=Linoleic acid metabolism
20  0.5328  1.0 0.0011  cfa02010=ABC transporters
22  0.5032  1.0 0.0015  cfa00280=Valine, leucine and isoleucine 
degradation
33  0.4444  1.0 0.0020  cfa04974=Protein digestion and 
absorption
15  0.5621  1.0 0.0022  cfa03440=Homologous recombination
14  0.5619  1.0 0.0028  cfa00650=Butanoate metabolism
35  0.4248  1.0 0.0035  cfa05323=Rheumatoid arthritis
16  0.5231  1.0 0.0043  cfa00350=Tyrosine metabolism
16  0.5231  1.0 0.0043  cfa05320=Autoimmune thyroid disease
12  0.5715  1.0 0.0044  cfa00592=alpha-Linolenic acid metabolism
15  0.5230  1.0 0.0054  cfa00760=Nicotinate and nicotinamide 
metabolism
21  0.4739  1.0 0.0054  cfa05144=Malaria
84  0.3666  1.0 0.0059  cfa04080=Neuroactive ligand-receptor 
interaction
16  0.4833  1.0 0.0091  cfa05143=African trypanosomiasis
12  0.5229  1.0 0.011   cfa00340=Histidine metabolism
14  0.4834  0.990.015   cfa05330=Allograft rejection
9   0.5618  1.0 0.015   cfa04614=Renin-angiotensin system
3   1.0 1   1.0 0.021   cfa00780=Biotin metabolism
35  0.3856  0.990.021   cfa00240=Pyrimidine metabolism
15  0.4443  0.990.030   cfa04940=Type I diabetes mellitus
11  0.4836  0.990.032   
cfa00563=Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
29  0.3854  0.980.032   cfa04512=ECM-receptor interaction
20  0.4150  0.980.034   cfa04622=RIG-I-like receptor signaling 
pathway
9   0.5032  0.990.037   cfa00100=Steroid biosynthesis
10  0.4837  0.990.042   cfa04950=Maturity onset diabetes of the 
young
7   0.5423  0.990.042   cfa03450=Non-homologous end-joining
6   0.5522  0.990.055   cfa00430=Taurine and hypotaurine 
metabolism
6   0.5522  0.990.055   cfa00790=Folate biosynthesis
22  0.3858  0.970.060   cfa05140=Leishmaniasis
12  0.4346  0.970.062   cfa00512=Mucin type O-Glycan 
biosynthesis
3   0.754   0.990.068   cfa00061=Fatty acid biosynthesis
3   0.754   0.990.068   cfa00232=Caffeine metabolism
23  0.3762  0.960.070   cfa04115=p53 signaling pathway
29  0.3664  0.960.071   cfa00564=Glycerophospholipid metabolism
8   0.4738  0.980.071   cfa00120=Primary bile 

[galaxy-user] special character $ gets converted to X in tool

2014-01-31 Thread Ketan Maheshwari
Hi,

In a test tool that I am working on, I need to enter text preceded by a $
sign to be interpreted as an environment variable by the underlying running
script. However, it seems that the $ sign gets converted to X when it gets
passed to the tool executable.

Is there a way to work around this or should I be doing something else to
pass environment variables via Galaxy tool UI.

Thanks,
Ketan
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Re: [galaxy-user] special character $ gets converted to X in tool

2014-01-31 Thread Peter Cock
On Fri, Jan 31, 2014 at 5:13 PM, Ketan Maheshwari
ketancmaheshw...@gmail.com wrote:
 Hi,

 In a test tool that I am working on, I need to enter text preceded by a $
 sign to be interpreted as an environment variable by the underlying running
 script. However, it seems that the $ sign gets converted to X when it gets
 passed to the tool executable.

 Is there a way to work around this or should I be doing something else to
 pass environment variables via Galaxy tool UI.

 Thanks,
 Ketan

This is a security feature I think - or it may just need escaping as \$

Since you are writing the script, why not pass in the environment
variable name without the dollar?

Peter
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[galaxy-user] February 2014 Galaxy Update Newsletter

2014-01-31 Thread Dave Clements
Hello all,

The 25th Edition of Galaxy Update is
outhttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02.
Highlights from the February 2014 Galaxy
Updatehttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02
 include:

   -

   42 new 
papershttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#New_Papers
   -

   Who's 
hiringhttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Who.27s_Hiring
   -

   Upcoming Eventshttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Events,
   including
   -

  Registration for GCC2014 opens February
10https://wiki.galaxyproject.org/GalaxyUpdates/2014_02#GCC2014:_June_30_-_July_2.2C_Baltimore
  -

  Registration for the Galaxy Australasia Workshop 2014 (GAW
2014)https://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Galaxy_Australasia_Workshop_2014_.28GAW_2014.29
   and GMOD Malaysia
2014https://wiki.galaxyproject.org/GalaxyUpdates/2014_02#GMOD_Malaysia_2014
are
  open.
  -

   Three new public Galaxy
servershttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#New_Public_Servers
   -

   January 2014 CloudMan
Releasehttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Galaxy_Distributions
   -

   New Galaxy Deployment
Cataloghttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Galaxy_Community_Hubs
and Community Log
Boardhttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Galaxy_Community_Hubs
entries
   -

   Tool Shed 
Contributionshttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#ToolShed_Contributions
   -

   Johns Hopkins and George Washington walk into a bar
...https://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Galaxy_is_now_at_Johns_Hopkins_and_GWU_.28and_Penn_State.21.29
   -

   Other Newshttps://wiki.galaxyproject.org/GalaxyUpdates/2014_02#Other_News

If you have anything you would like to see in the next *Galaxy Update
https://wiki.galaxyproject.org/GalaxyUpdates*, please let us know.

Dave Clements and the Galaxy Teamhttps://wiki.galaxyproject.org/GalaxyTeam

-- 
http://galaxyproject.org/
http://getgalaxy.org/
http://usegalaxy.org/
http://wiki.galaxyproject.org/
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[galaxy-user] GATK - Base recalibrator

2014-01-31 Thread Bolduc, Francois
Hi all,

I'm trying to call variants using GATK best practices workflow. So after 
performing Realigner Target Creator step, one should jump on Base Recalibrator 
step. Unfortunatly, I don't see this program under GATK tab. Is there a way to 
perform this step in Galaxy?

There are the Count covariates and Table recalibration steps. Is a combination 
of these two steps is equivalent to Base recalibrator?

Thank you for your help and time.




Frank
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Re: [galaxy-user] GATK - Base recalibrator

2014-01-31 Thread Geraldine Van der Auwera
Hi Frank,

Yes, that's equivalent; BaseRecalibrator is a single tool that combines
those two steps in more recent versions. The version of GATK in Galaxy (1.6
afaik) is a pretty old one. I would strongly recommend using the most
recent version of GATK (2.8) as it will give you better results.

Good luck
Geraldine


On Fri, Jan 31, 2014 at 1:51 PM, Bolduc, Francois 
francois.bol...@usherbrooke.ca wrote:

  Hi all,

 I'm trying to call variants using GATK best practices workflow. So after
 performing Realigner Target Creator step, one should jump on Base
 Recalibrator step. Unfortunatly, I don't see this program under GATK tab.
 Is there a way to perform this step in Galaxy?

 There are the Count covariates and Table recalibration steps. Is a
 combination of these two steps is equivalent to Base recalibrator?

 Thank you for your help and time.




 Frank

 ___
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-- 
Geraldine A. Van der Auwera, Ph.D.
Bioinformatics Scientist
Broad Institute
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Re: [galaxy-user] problem with scientific notation sorting and filtering in galaxy

2014-01-31 Thread Dave Clements
Hi Douglas,

I encourage you and anyone else that has encountered this error to up-vote
this issue in the Galaxy Trello board:

  https://trello.com/c/QZlmb0Yf

That will help us prioritize work.  Thanks for reporting this.

Dave C


On Thu, Jan 30, 2014 at 10:24 AM, Douglas Cavener d...@psu.edu wrote:

 I have found that numbers in scientific notation (e.g 9.8E-9) are
 incorrectly sorted or filtered by Galaxy. Looks like the negative exponent
 is ignored and so if you sorting something like P values (see example
 below) then you find these ordered above P=1 instead at the other end of
 the list where P is near zero. In the example below a KEGG list is ordered
 on column 5 in asending order by galaxy. If you scroll to the bottom of the
 list, however, you will see the smallest numbers all of which are in
 scientific notation. I should note that I can can copy this into excel and
 excel will properly sort scientific notation. So this seems to be a
 specific problem with Galaxy. I found this problem with both filtering and
 sorting functions of Galaxy.
 Doug Cavener

 410.53261.00.025cfa04146=Peroxisome390.5325 
 1.00.038cfa04640=Hematopoietic
 cell lineage290.59131.00.044cfa00980=Metabolism of xenobiotics by
 cytochrome P450 220.63101.00.16cfa00140=Steroid hormone biosynthesis28
 0.56191.00.27cfa05204=Chemical carcinogenesis 
 110.8531.00.33cfa00511=Other
 glycan degradation250.57161.00.52cfa00600=Sphingolipid metabolism 9
 0.9021.00.74cfa00740=Riboflavin 
 metabolism230.58141.00.79cfa00380=Tryptophan
 metabolism 150.6861.00.96cfa04977=Vitamin digestion and absorption22
 0.56171.00.00017cfa00071=Fatty acid metabolism 
 220.56171.00.00017cfa05150=Staphylococcus
 aureus infection260.53271.00.00017cfa00982=Drug metabolism - cytochrome
 P450 490.44421.00.00018cfa04142=Lysosome560.42471.00.00024cfa04630=Jak-STAT
 signaling pathway 340.48351.00.00026cfa03320=PPAR signaling pathway130.685
 1.00.00027cfa00531=Glycosaminoglycan degradation 
 200.56211.00.00043cfa04975=Fat
 digestion and absorption220.54241.00.00043cfa00830=Retinol metabolism 19
 0.56201.00.00053cfa00565=Ether lipid 
 metabolism140.6.00.00092cfa00591=Linoleic
 acid metabolism 200.53281.00.0011cfa02010=ABC transporters220.50321.0
 0.0015cfa00280=Valine, leucine and isoleucine degradation 330.1.0
 0.0020cfa04974=Protein digestion and 
 absorption150.56211.00.0022cfa03440=Homologous
 recombination 140.56191.00.0028cfa00650=Butanoate metabolism350.42481.0
 0.0035cfa05323=Rheumatoid arthritis 160.52311.00.0043cfa00350=Tyrosine
 metabolism160.52311.00.0043cfa05320=Autoimmune thyroid disease 120.57151.0
 0.0044cfa00592=alpha-Linolenic acid 
 metabolism150.52301.00.0054cfa00760=Nicotinate
 and nicotinamide metabolism 210.47391.00.0054cfa05144=Malaria840.36661.0
 0.0059cfa04080=Neuroactive ligand-receptor interaction 
 160.48331.00.0091cfa05143=African
 trypanosomiasis120.52291.00.011cfa00340=Histidine metabolism 140.48340.99
 0.015cfa05330=Allograft rejection90.56181.00.015cfa04614=Renin-angiotensin
 system 31.011.00.021cfa00780=Biotin 
 metabolism350.38560.990.021cfa00240=Pyrimidine
 metabolism15 0.44430.990.030cfa04940=Type I diabetes mellitus110.48360.99
 0.032cfa00563=Glycosylphosphatidylinositol(GPI)-anchor biosynthesis 290.38
 540.980.032cfa04512=ECM-receptor 
 interaction200.41500.980.034cfa04622=RIG-I-like
 receptor signaling pathway 90.50320.990.037cfa00100=Steroid biosynthesis10
 0.48370.990.042cfa04950=Maturity onset diabetes of the young 70.54230.99
 0.042cfa03450=Non-homologous end-joining60.55220.990.055cfa00430=Taurine
 and hypotaurine metabolism 60.55220.990.055cfa00790=Folate biosynthesis22
 0.38580.970.060cfa05140=Leishmaniasis 120.43460.970.062cfa00512=Mucin
 type O-Glycan biosynthesis30.7540.990.068cfa00061=Fatty acid biosynthesis
 30.7540.990.068cfa00232=Caffeine metabolism230.37620.960.070cfa04115=p53
 signaling pathway 290.36640.960.071cfa00564=Glycerophospholipid metabolism
 80.47380.980.071cfa00120=Primary bile acid biosynthesis 
 200.38590.960.074cfa00561=Glycerolipid
 metabolism190.38570.960.076cfa04612=Antigen processing and presentation 48
 0.34740.950.076cfa05152=Tuberculosis180.38530.960.077cfa00510=N-Glycan
 biosynthesis 21.011.00.077cfa00290=Valine, leucine and isoleucine
 biosynthesis160.39520.960.079cfa00500=Starch and sucrose metabolism 160.39
 520.960.079cfa04621=NOD-like receptor signaling 
 pathway120.41490.960.080cfa00410=beta-Alanine
 metabolism 70.47390.970.093cfa00604=Glycosphingolipid biosynthesis -
 ganglio series330.34720.940.094cfa05146=Amoebiasis 
 80.44410.960.097cfa00900=Terpenoid
 backbone biosynthesis40.57150.980.099cfa00460=Cyanoamino acid metabolism 4
 0.57150.980.099cfa00750=Vitamin B6 
 metabolism120.40510.950.10cfa00052=Galactose
 metabolism 120.40510.950.10cfa00983=Drug metabolism - other enzymes90.4346
 0.960.10cfa00040=Pentose and glucuronate interconversions 
 50.50320.970.11cfa04122=Sulfur
 relay system260.35700.930.12cfa04064=NF-kappa B signaling 

Re: [galaxy-user] special character $ gets converted to X in tool

2014-01-31 Thread Ketan Maheshwari
Thanks!

Just wanted to report that \$ does not work; \ adds one more X.


On Fri, Jan 31, 2014 at 11:29 AM, Peter Cock p.j.a.c...@googlemail.comwrote:

 On Fri, Jan 31, 2014 at 5:13 PM, Ketan Maheshwari
 ketancmaheshw...@gmail.com wrote:
  Hi,
 
  In a test tool that I am working on, I need to enter text preceded by a $
  sign to be interpreted as an environment variable by the underlying
 running
  script. However, it seems that the $ sign gets converted to X when it
 gets
  passed to the tool executable.
 
  Is there a way to work around this or should I be doing something else to
  pass environment variables via Galaxy tool UI.
 
  Thanks,
  Ketan

 This is a security feature I think - or it may just need escaping as \$

 Since you are writing the script, why not pass in the environment
 variable name without the dollar?

 Peter




-- 
Ketan
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