[galaxy-user] Fwd: Question about FTP

2011-03-07 Thread Cathy Riemer
- Forwarded message from Noushin Ghaffari noushin.ghaff...@gmail.com -

Date: Mon, 7 Mar 2011 09:09:13 -0600
From: Noushin Ghaffari noushin.ghaff...@gmail.com
To: dbad...@bio.cse.psu.edu
Subject: Question about FTP

Dear Galaxy team,

Firstly, thank you very much for the great tools.

I am working on a large dataset and need to upload it to Galaxy via FTP. I
used

ftp://main.g2.bx.psu.edu

but I cannot login. I used my email, my public name on Galaxy and just
simple my name, but none of them worked. Can you please help me to know how
can I login to upload my file?

Here is my inofrmation:

email: noushin.ghaff...@gmail.com

public name: me-on-galaxy

I appreciate you help and time in advance.

Noushin

- End forwarded message -
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[galaxy-user] BED-to-GFF: option for gff3 format

2011-03-07 Thread Karen Tang

Hi Galaxy developers,

Any plans on adding a gff3 format option to BED-to-GFF?

I found a BED-to-GFF3 converter at the Rätsch lab's instance 
of Galaxy (http://galaxy.tuebingen.mpg.de/). Any plans on 
making this converter more widely available?


Karen :)
Dept of Plant Biology
University of Minnesota___
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Re: [galaxy-user] GTF-to-GFF3

2011-03-07 Thread Karen Tang

Hi Galaxy developers,

Any plans on adding a GTF-to-GFF3 format conversion?

This converter is at the Rätsch lab's instance of Galaxy 
(http://galaxy.tuebingen.mpg.de/). Could it be made more

widely available?

Karen :)
Dept of Plant Biology
University of Minnesota___
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[galaxy-user] Bowtie Job doesn't start

2011-03-07 Thread Felix Hammer

Hi,
just trying to use Bowtie, but the job does not start.
Is there somthing wrong with the server at the moment?
Felix
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[galaxy-user] irc channel for galaxy

2011-03-07 Thread George Marselis
Hey guys, 

Is there an irc channel for galaxy? I googled for galaxy irc but all I
see in the first fifty results are entries for the samsung galaxy (which
is an excellent phone ;)

Best Regards,

George Marselis, systems administrator
Building #2, Level 4, room 4327
Computational Bioscience Research Center, KAUST
Land: +966-2-808-2944, Mobile: +966-56-321-7714, Skype: project2501a


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Re: [galaxy-user] Fwd: Need a generous help regarding uploading

2011-03-07 Thread Jennifer Jackson

Hi Vasu,

Very sorry to hear that you are still having problems. Have you been 
able to do the update? It was not clear from your email.


The latest is: http://bitbucket.org/galaxy/galaxy-central/wiki/GetGalaxy

new:  % hg clone http://www.bx.psu.edu/hg/galaxy galaxy-dist
upgrade:  % hg pull -u -r ca0c4ad2bb39

When you reply, could you note which revision you are using (perhaps you 
are pulling from galaxy-central instead?)


Thanks for helping us to diagnose the problem,

Jen
Galaxy team

On 1/3/11 12:05 PM, vasu punj wrote:

NO I am still unable to get it through. Any help in this regard will be
apprceiated.
Vasu

--- On *Mon, 1/3/11, Jennifer Jackson /j...@bx.psu.edu/* wrote:


From: Jennifer Jackson j...@bx.psu.edu
Subject: Re: [galaxy-user] Fwd: Need a generous help regarding uploading
To: vasu punj pu...@yahoo.com
Cc: galaxy-u...@bx.psu.edu galaxy-u...@bx.psu.edu
Date: Monday, January 3, 2011, 1:59 PM

Hello Vasu,

Are you still having problems? Or did this other reply help?

http://gmod.827538.n3.nabble.com/Bam-file-after-Tophat-run-td2089789.html#a2092204

If the problem is persistent, Galaxy needs to be updated to get better
UI support for downloading BAM files.

Hopefully the problem can be resolved, but please let us know if you
need more help.

Thanks!

Jen
Galaxy team


On 12/15/10 12:23 PM, vasu punj wrote:
  I have submited your email to share job 31 and 25 are the Tophat runs
  which I am trying to save as bam files from Floppy icon.
  The job is run on our University cluster. I have alos enclosed screen
  shot for your ready reference.
  vasu
 
  --- On *Wed, 12/15/10, Jennifer Jackson /j...@bx.psu.edu



  Hello Asifullah,
 
  It would be helpful if you would be able to share your history.
Please
  note which exact datasets (by number) you are having problems saving.
 
  Use Options - Share or Publish - click on the button to Share via
  link
  and then email that to me.
 
  Thank you,
 
  Jen
  Galaxy team
 
  On 12/15/10 7:56 AM, vasu punj wrote:
   I have done the same it gav eme bam index file when i removed the
   extension index or as such it come out to be not right bam file
  format
   as recognized by various tools
   Thanks
   Date: Wednesday, December 15, 2010, 9:52 AM
  
   Hello Asifullah,
  
   Click on the save icon for the dataset to initiate a
download. This
   icon is shaped like a little purple disk on the very left side
of the
   dataset's box in the right history pane.
  
   Hopefully this is helpful,
  
   Thank you,
  
   Jen
   Galaxy team
  
   On 12/15/10 7:47 AM, vasu punj wrote:
Hi,
Can we save Bam file from Tophat out put if so how can we do that
   please.
Thanks.



Date: Wednesday, December 15, 2010, 9:38 AM
   
Hello Asifullah,
   
The Galaxy tool Get Data - Upload File has instructions for
uploading
data to the FTP server. We recommend using the 3rd party utility
FileZilla as a desktop FTP client.
   
http://file-zilla.com/
   
Hopefully this will get you started,
   
Best!
   
Jen
Galaxy team
   
ps: In the future, if you could send questions directly to
galaxy-u...@bx.psu.edu



 *Subject: **Need a generous help regarding uploading*

 Dear All,

 I am in trouble to upload 1.2Gb illumine data on Galaxy
page for
 subsequent assembling and analysis steps. I am basically a
   biologist
 and new regarding utility of modern bioinformatics tools.
  some one
 have suggested to upload first your data on a http web page
   then it
 would be easy to be uploaded on GALaxy. But it is also not
   possible
 for me as having little computer knowledge. So could some one
help me
 generously regarding these issue. I will highly appreciate
his/her help.

 Regards
 Asifullah Khan



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Re: [galaxy-user] Bowtie Job doesn't start

2011-03-07 Thread Nate Coraor
Felix Hammer wrote:
 Hi,
 just trying to use Bowtie, but the job does not start.
 Is there somthing wrong with the server at the moment?

Hi Felix,

It looks like the reservation on the cluster which runs bowtie jobs
isn't working at the moment and there's some contention for resources.
I've reported the bug to the folks who maintain this cluster and
hopefully it'll be resolved shortly.

--nate

 Felix
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 please use the interface at:
 
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Re: [galaxy-user] Adding the Hydra genome to Galaxy

2011-03-07 Thread Jennifer Jackson

Thanks Rob,

This definitely helps - we appreciate the additional info. This is on 
our high-priority list and Kelly will get back to you if we have more 
questions.


Best,

Jen

On 3/7/11 1:23 PM, Rob Steele wrote:

Hi Jen,
The easiest way to refer to this genome would be Hydra_JCVI. This way we
know it is the JCVI assembly (ABRM). ACZU is a separate
assembly that was done at Berkeley. Including the two mitochondrial
genomes would be a good idea. The many WGS submissions under
ABRM are the scaffolds/contigs from the JCVI assembly. Because
we were unable to obtain long-insert clones (e.g. BACs), the assembly is
more fragmented than is typical for a metazoan genome assembly, and thus
it has more scaffolds/contigs than is typical.

Cheers,
Rob

Rob Steele, Ph.D.
Professor
D240 Medical Sciences I
Department of Biological Chemistry
School of Medicine
University of California, Irvine
Irvine, CA 92697-1700

phone: 949-824-7341
e-mail: reste...@uci.edu
fax: 949-824-2688
web: http://polyp.biochem.uci.edu/wiki/index.php/Main_Page


On 3/7/11 1:12 PM, Jennifer Jackson wrote:

Hi Rob,

The project ABRM links to several thousand individual WGS
submissions. Are there scaffolds or some type of assembled sequence
available? Or, perhaps there an alternate source?

Also, did you want us to include the two mitochondrial genomes from
NCBI in the main assembly (once we have it?). This is common for us to
do, but not required.

And finally, if there is a short-hand name for the genome that you and
the Hydra community use, we can assign that as the short-label for the
genome. Otherwise, we just use the Genus_species_source_date for the
label. Some way to distinguish from ACZU seems important.

Thanks Rob, we look forward to your feedback.

Jen

On 3/6/11 4:21 PM, Rob Steele wrote:

Hi Jen,
How are things progressing with the Hydra genome?

Cheers,
Rob

Rob Steele, Ph.D.
Professor
D240 Medical Sciences I
Department of Biological Chemistry
School of Medicine
University of California, Irvine
Irvine, CA 92697-1700

phone: 949-824-7341
e-mail: reste...@uci.edu
fax: 949-824-2688
web: http://polyp.biochem.uci.edu/wiki/index.php/Main_Page


On 2/15/11 6:17 AM, Jennifer Jackson wrote:

Hello Rob,

This genome is being worked on right now and should be available soon.
Please feel free to check back a few weeks again for an update if you
do not see it by then.

Thanks!

Jen
Galaxy team

On 2/14/11 3:20 PM, Rob Steele wrote:

Hi Jen et al.,
Sorry to be a pest, but do you have an estimate for when the Hydra
genome will be added to Galaxy?

Cheers,
Rob

Rob Steele, Ph.D.
Professor
D240 Medical Sciences I
Department of Biological Chemistry
School of Medicine
University of California, Irvine
Irvine, CA 92697-1700

phone: 949-824-7341
e-mail: reste...@uci.edu
fax: 949-824-2688
web: http://polyp.biochem.uci.edu/wiki/index.php/Main_Page


On 1/3/11 12:53 PM, Jennifer Jackson wrote:

Hello Rob,

We will add this to our to-do list for new genomes. Thanks for
sending
the Genbank information!

Next time, if you could send requests to galaxy-user, that would be
very helpful for the team.

Best,

Jen
Galaxy team

On 1/3/11 12:37 PM, Rob Steele wrote:

Hi Jennifer,
Would it be possible to get the Hydra genome assembly added to
Galaxy?
It has been published and is available in GenBank under accession
number
ABRM.

Cheers,
Rob

Rob Steele, Ph.D.
Professor
D240 Medical Sciences I
Department of Biological Chemistry
School of Medicine
University of California, Irvine
Irvine, CA 92697-1700

phone: 949-824-7341
e-mail: reste...@uci.edu
fax: 949-824-2688
web: http://polyp.biochem.uci.edu/wiki/index.php/Main_Page









--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org
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