[galaxy-user] question about sorting barcoded sequences

2011-10-17 Thread Qingquan Liu
Hi, I have an Illumina HiSeq lane of sequences, in which I input multiple samples with 5-prime 6 bp barcodes. The barcodes were added in a way so that the barcodes are the first 6 bp in the reads. What I need to do is to sort my reads according to the barcodes, then clip off the barcodes and use

[galaxy-user] Data files for Gencode Partition tool

2011-10-17 Thread Harald Dahle
Hello! I tried asking this at the galaxy-dev list a while back, but figure it might more rightly belong on this list. I am trying to configure the Gencode Partition tool on our local installation of Galaxy. However, I am not able to find any information about what sort of files it should

[galaxy-user] Memory issues while using Galaxy

2011-10-17 Thread prashant singh
Hi We have installed Galaxy on a local machine (Mac pro 2 x 2.4 gHz quad core Intel Xeon with 32 GB RAM). We wish to use dynamic trimmer in order to trim the data and while downloading a groomed file from galaxy, it runs out of memory. Please advise. Sent from my iPhone