[galaxy-user] Finding Galaxy version

2011-12-13 Thread Paul-Michael Agapow
A perhaps obvious question: how do I work out what version of Galaxy an
instance is?

 

This has come  up a few times because of apparent bugs and different
behaviour across various development and production instances. Now if
you're installed straight from the repo, you can use hg log for the
repo number. But that doesn't work if it's been installed in another
way, say from a tarball or via another versioning system (we use git to
synchronize tool development to a production server). 

 

Simple solution?

 



Paul Agapow (paul-michael.aga...@hpa.org.uk)

Bioinformatics, Health Protection Agency

 



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[galaxy-user] Help

2011-12-13 Thread Giuseppe Petrosino
Hi,
I read on Readme for MACS that: For the experiment with several
replicates, it is recommended to concatenate several ChIP-seq treatment
files into a single file.
Now, I have illumina ChipSeq data: two files for IP samples and two files
for Control samples. Is It right to use Concatenate datasets (text
manipulation) and then use MACS for the peaks calling?
Thank you so much.

Giuseppe
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[galaxy-user] SNPEff - Reports Gain of Stop Codon as FRAME_SHIFT in some cases

2011-12-13 Thread John David Osborne
Hi Pablo,

Correct me if I am wrong, but I believe that SNPEff doesn't check the inserted 
sequence when computing the effect of an insertion?

For instance an insertion of TTTA will be reported as a frameshift mutation. In 
the majority of cases this is fine, however if the current reading frame from 
the ORF annotation is the negative strand and the correct frame is selected the 
new codon will be:
TAA T…

The TAA is actually a stop codon, so the real effect of this insert should be 
insertion of a STOP CODON, not a frame shift in this instance. This is minor I 
know, but I though to report and make people aware.

I am using version 2.02 of SNPEff, but I don't see this fix reported up to 2.04.

 -John


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