[galaxy-user] upgrade script

2012-02-21 Thread Victor Ruotti
Hi,
I'm trying to use the upgrade script  given that the current schema is now 
version 90.
I get the following error.
Can anyone point me out, maybe there is something to do before upgrading? 

thanks in advance.
Victor



trying to start galaxy I get 
Traceback (most recent call last):
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/web/buildapp.py, line 82, 
in app_factory
app = UniverseApplication( global_conf = global_conf, **kwargs )
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/app.py, line 32, in 
__init__
create_or_verify_database( db_url, kwargs.get( 'global_conf', {} ).get( 
'__file__', None ), self.config.database_engine_options )
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/model/migrate/check.py, 
line 105, in create_or_verify_database
% ( db_schema.version, migrate_repository.versions.latest, config_arg ) )
Exception: Your database has version '90' but this code expects version '79'.  
Please backup your database and then migrate the schema by running 'sh 
manage_db.sh -c ./universe_wsgi.runner.ini upgrade'.
Removing PID file runner0.pid

--trying to upgrade 

sh manage_db.sh -c ./universe_wsgi.runner.ini upgrade 
Traceback (most recent call last):
  File ./scripts/manage_db.py, line 63, in module
main( repository=repo, url=db_url )
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/shell.py,
 line 150, in main
ret = command_func(**kwargs)
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/api.py,
 line 221, in upgrade
return _migrate(url, repository, version, upgrade=True, err=err, **opts)
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/api.py,
 line 327, in _migrate
changeset = schema.changeset(version)
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/schema.py,
 line 173, in changeset
changeset = self.repository.changeset(database, start_ver, version)
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/repository.py,
 line 170, in changeset
changes = [self.version(v).script(database, op) for v in versions]
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/repository.py,
 line 145, in version
return self.versions.version(*p, **k)
  File 
/opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/version.py,
 line 125, in version
return self.versions[VerNum(vernum)]
KeyError: VerNum(90)

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Re: [galaxy-user] upgrade script

2012-02-21 Thread Greg Von Kuster
Hello Victor,

The following statement in your log is concerning because it looks like 
something in your environment got corrupted in some way.

Exception: Your database has version '90' but this code expects version '79'.

Clean database schema migration upgrades go from smaller to larger numbers 
(e.g., 89 - 90, 90 - 91, etc).  Your migrate_versions.version database table 
column has a value of 90, but your ~/lib/galaxy/model/migrate/versions 
sub-directory has scripts only up to 0079... (or so it seems).  Not sure how 
best to fix the problem...

Greg Von Kuster

On Feb 21, 2012, at 2:21 PM, Victor Ruotti wrote:

 Hi,
 I'm trying to use the upgrade script  given that the current schema is now 
 version 90.
 I get the following error.
 Can anyone point me out, maybe there is something to do before upgrading? 
 
 thanks in advance.
 Victor
 
 
 
 trying to start galaxy I get 
 Traceback (most recent call last):
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/web/buildapp.py, line 82, 
 in app_factory
app = UniverseApplication( global_conf = global_conf, **kwargs )
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/app.py, line 32, in 
 __init__
create_or_verify_database( db_url, kwargs.get( 'global_conf', {} ).get( 
 '__file__', None ), self.config.database_engine_options )
  File /opt/galaxy/galaxy-dist-original/lib/galaxy/model/migrate/check.py, 
 line 105, in create_or_verify_database
% ( db_schema.version, migrate_repository.versions.latest, config_arg ) )
 Exception: Your database has version '90' but this code expects version '79'. 
  Please backup your database and then migrate the schema by running 'sh 
 manage_db.sh -c ./universe_wsgi.runner.ini upgrade'.
 Removing PID file runner0.pid
 
 --trying to upgrade 
 
 sh manage_db.sh -c ./universe_wsgi.runner.ini upgrade 
 Traceback (most recent call last):
  File ./scripts/manage_db.py, line 63, in module
main( repository=repo, url=db_url )
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/shell.py,
  line 150, in main
ret = command_func(**kwargs)
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/api.py,
  line 221, in upgrade
return _migrate(url, repository, version, upgrade=True, err=err, **opts)
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/api.py,
  line 327, in _migrate
changeset = schema.changeset(version)
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/schema.py,
  line 173, in changeset
changeset = self.repository.changeset(database, start_ver, version)
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/repository.py,
  line 170, in changeset
changes = [self.version(v).script(database, op) for v in versions]
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/repository.py,
  line 145, in version
return self.versions.version(*p, **k)
  File 
 /opt/galaxy/galaxy-dist-original/eggs/sqlalchemy_migrate-0.5.4-py2.6.egg/migrate/versioning/version.py,
  line 125, in version
return self.versions[VerNum(vernum)]
 KeyError: VerNum(90)
 
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Re: [galaxy-user] ftp Server unavailable

2012-02-21 Thread Jennifer Jackson

Hello Florian,

Hopefully you have noticed that the service is up now, but if not, 
please try your FTP load again and let us know if you continue to have 
problems.


Best,

Jen
Galaxy team

On 2/20/12 3:04 AM, Florian Peschke wrote:



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http://galaxyproject.org/wiki/Support
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Re: [galaxy-user] about primer/adaptor removal?

2012-02-21 Thread Jennifer Jackson

Hello Arthur,

The tool NGS: QC and manipulation - FASTX-Toolkit - Clip will remove 
specified 3' adapter sequence.


Other Trim tools in the sections NGS: QC and manipulation - Generic 
FASTQ manipulation  FASTX-Toolkit may be of interest, if you want to 
clip by length/quality.


Hopefully this provides you with some options,

Best,

Jen
Galaxy team

On 2/20/12 7:32 AM, Hao Zheng wrote:

Hi,

Does galaxy provide primer/adaptor removal for NGS (illumina) reads?

Thanks.

Arthur




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--
Jennifer Jackson
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http://galaxyproject.org/wiki/Support
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[galaxy-user] Cufflinks related problem

2012-02-21 Thread Bomba Dam
Dear Noa,

Can you please  tell me the parameters that I should keep while mapping
bacterial transcripts using cuffkins. I have kept the default parameters as
in Cufflinks and used my custom genome annotation  in gff3 format. The
cufflinks seems to work Ok but all the FPKM values in these files are zero.
As suggested by other users I have checked the correctness of my GFF3
files. The corresponding fasta file was used for mapping the transcripts
using Bowtie. Are there any special trick for mapping bacterial
transcriptome.

regards,

Bomba
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Re: [galaxy-user] Megablast

2012-02-21 Thread Jennifer Jackson

Hello Scott,

For #1, option -p:

Here is a link to some megablast parameter documentation online:
http://www.ncbi.nlm.nih.gov/staff/tao/URLAPI/megablast.html#3
(the primary paper for the Galaxy tool is noted at the bottom of the 
tool form, but this is convenient)


Quote:

Table 3.30 Parameter -p
FunctionSpecifies the percentage identity cut-off
Default 0
Input format[Real]
Example To set percent id cutoff to 75%, use: -p 75
Note: The input value range is between 0 and 100, with 0 meaning no 
cutoff. It only works on the aligned region or individual HSPs.


For #2, there are a few ways to interpret filter. If you mean will 
megablast consider the adapter part of the sequence in calculations, the 
answer is that it does for some and doesn't for others. The part of the 
sequence that is adapter wouldn't align to the genome, and percent 
identity is only based on HSPs (high scoring pairs - one part of the 
pair is the DNA query and the other is the genome target, for that 
alignment region only). So, adapter sequence wouldn't be involved in 
percent identify calculations (or be expected to!). But, these unaligned 
regions could become a problem if coverage or certain other statistics 
were part of your analysis. Learning about the statistics you choose to 
use, to see if query length is part of the calculation, will let you 
know if clipping is necessary. If important, removing adapters can be 
done with tools in NGS: QC and manipulation (perform a tool search on 
keywords trim or clip.



Best,

Jen
Galaxy team

On 2/20/12 4:59 PM, Scott Tighe wrote:

Hi Galaxy users

When Magablasting

1)what does the identity value -p mean ...is it percent identity?
I want my megablast results to be reported form only a 100% match. I do
not see a place for % alinement concordance.
2) form my Illumina Hiseq reads, are the adaptor sequences filtered
during the filter step?

Scott tighe

--2
Scott Tighe
Advanced Genome Technology Lab
Vermont Cancer Center at the University of Vermont
149 Beaumont Avenue
Health Science Research Bd RM 305
Burlington Vermont USA 05405
lab  802-656-AGTC (2482)
cell 802-999-



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[galaxy-user] merging databases

2012-02-21 Thread De Gendt, Karel
Hi!

Here's a really simple question, but I somehow can't find an easy answer in 
Galaxy.
I want to merge two fastq files (they contain 2 sequencing runs of the same 
library, and I want to pool them).
How do I do that in Galaxy?
Thanks!

Karel



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