[galaxy-user] tophat deletions output

2012-08-21 Thread i b
dear all, how can we use the tophat deletions output? e.g. if I want to see and conpare between two samples if a specific gene or transcript had been deleted, how can I use this output? is visualisation enough? thanks, ib ___ The Galaxy User

[galaxy-user] Files uploaded via FTP - 530 Sorry, the maximum number of clients (3) for this user are already connected.

2012-08-21 Thread Julie Rodor
Hi, I am trying to upload new files to galaxy via FTP using Filezilla. I have this error message: 530 Sorry, the maximum number of clients (3) for this user are already connected. What can I do? Julie -- The University of Edinburgh is a charitable body, registered in Scotland, with

[galaxy-user] cuffdiff sample values assigned

2012-08-21 Thread Jennifer Jackson
On 8/21/12 4:33 AM, i b wrote: Thanks Jen, useful link. But I did not understand one thing. I have the following FPKM in cufflinks for two samples: s1 (untreated): 1234106 s2 (treated): 159713 cuffdiff of the two samples gives me the following values: value_1:5.4 value_2:20.9 and it is

Re: [galaxy-user] run Bowtie to estimate Mean Inner Distance between Mate Pairs

2012-08-21 Thread rsharris
Howdy Jianguang, There's a more complete description of the SAM format in The Sequence Alignment/Map format and SAMtools, Li et al, Bioinformatics (2009). And you can find the latest specification for the format at samtools.sourceforge.net . In the spec, the terminology for the ISIZE field has

[galaxy-user] Aggregate collections of files (example: Heatmaps)

2012-08-21 Thread Ted Goldstein
I am developing several tools that will need to read and write multiple data files at once. For example, Eisen Cluster produces a heatmap which consists of three files: a .cdt file, .atr file and a gtr file which are the underlying heatmap and the array tree and the gene tree. All three

Re: [galaxy-user] Files uploaded via FTP - 530 Sorry, the maximum number of clients (3) for this user are already connected.

2012-08-21 Thread Jennifer Jackson
Hi Julie, This was mostly likely an issue on our side. Please try to FTP again and let us know if you continue to have problems. Our apologies for the inconvenience, Jen Galaxy team On 8/21/12 3:23 AM, Julie Rodor wrote: Hi, I am trying to upload new files to galaxy via FTP using

[galaxy-user] FTP upload problem

2012-08-21 Thread Fatih Ozsolak
Hi, I am trying to upload 12 fastq files to Galaxy via FTP. The FTP is randomly loosing connection, caussing interruption of the upload. Can you let me know what is wrong? Thanks, fatih ___ The Galaxy User list should be used for the

[galaxy-user] How to find the alternatively spliced segment of genes in Cuffdiff output

2012-08-21 Thread Du, Jianguang
Dear All, I have run programs from Tophat to Cuffdiff of Galaxy to look for the difference in alternative splicing events between cell types. However I do not know how to find the detail information (such as the sequence and the genomic coordinates) of the alternatively spliced part of a

Re: [galaxy-user] Aggregate collections of files (example: Heatmaps)

2012-08-21 Thread Peter Cock
On Tue, Aug 21, 2012 at 4:46 PM, Ted Goldstein t...@soe.ucsc.edu wrote: I am developing several tools that will need to read and write multiple data files at once. For example, Eisen Cluster produces a heatmap which consists of three files: a .cdt file, .atr file and a gtr file which are

[galaxy-user] New reference genome in Bowtie

2012-08-21 Thread Sarah Marcus
Dear All, I was wondering if it would be possible to add Mycobacterium tuberculosis H37Rv as a reference genome to the list of built-in indexes in the Map with Bowtie for Illumina (version 1.1.2) tool. Thank you, Sarah ___ The Galaxy User

[galaxy-user] How to upload multiple files......

2012-08-21 Thread Neil.Burdett
Hi, I've recently installed galaxy. I was trying to find how to upload multiple files without the need to compress them into a zip/gz file first. Is this possible? As the Get Data option allows only one file at a time. I searched the galaxy archive mailing list but didn't find anything

Re: [galaxy-user] FTP upload problem

2012-08-21 Thread Richard Mark White
Hi, I have been having FTP issues for the past several days.  I am using Filezilla.  The upload starts, then stops after about 32MB and the connection closes and tries to reopen, then closes again.  I also tried the command line ftp client in win7 with same results. rich

[galaxy-user] LimitInternalRecursion

2012-08-21 Thread Adhemar
Hi, There's this error message in my apache log: [error] Request exceeded the limit of 10 subrequest nesting levels due to probable confguration error. Use 'LimitInternalRecursion' to increase the limit if necessary. Use 'LogLevel debug' to get a backtrace., referer: ... When I turn the apache

[galaxy-user] file upload renamed

2012-08-21 Thread Neil.Burdett
When uploading a file using “Get Data” it seems the file is renamed to dataset_’id’.dat in ~/database/files/000/. Is it possible for the file to keep its name rather than being renamed? It seems this is being done because of the line