Hi Jianguang,
Using known reference annotation ("Use Own Junctions", etc.) is not a
part of the example RNA-seq tutorial our team has published for this
type of analysis. That doesn't mean that it cannot be used, but that how
it could or should be used probably needs to be tested to see if the
Dear All,
I have two more questions about settings for Tophat.
My aim is to look for the defferential splicing events between cell types.
After I checked "Use Own Junctions", three more options came out:
1) "Use Gene Annotation Model"
2) "Use raw Junctions"
3) "Only look for supplied juncti
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