[galaxy-user] Tools won't go away even after deleting it from tool_conf.xml

2012-09-06 Thread Makis Ladoukakis
Dear Galaxy users, Has anyone experienced this problem before? In my local instance of Galaxy on a server I've deleted the path of almost all sections with their corresponding tools from tool_conf.xml (wanting to leave my own customized section) but after a restart and a new run of the run.sh

Re: [galaxy-user] Tools won't go away even after deleting it from tool_conf.xml

2012-09-06 Thread Hans-Rudolf Hotz
moving to galaxy-dev this is probably related to this issue: http://dev.list.galaxyproject.org/problems-with-labels-in-the-tool-box-and-order-of-the-tools-td4655985.html Hence, try deleting integrated_tool_panel.xml first before you do a restart. Regards, Hans On 09/06/2012 01:29 PM,

[galaxy-user] Tophat settings

2012-09-06 Thread Du, Jianguang
Dear All, I am not so sure about two Tophat settings. Please help. 1) Number of mismatches allowed in the initial read mapping Based on the documantation, my understanding is: the reads are re-aligned to transcriptome/genome if the mismatches in the initial alignment is more than the set

Re: [galaxy-user] Please help to understand the square root of Jensen-Shannon divergence

2012-09-06 Thread Jennifer Jackson
Hello Jianguang, Fold is included in the Cuffdiff output. Section Differential expression tests, first file, column #9. http://cufflinks.cbcb.umd.edu/manual.html Hopefully this helps, Jen Galaxy team On 9/4/12 1:16 PM, Du, Jianguang wrote: Dear All, I am looking for the differential

Re: [galaxy-user] Please help to understand the square root of Jensen-Shannon divergence

2012-09-06 Thread Du, Jianguang
Hi Jen, Thank you for your answer. However, the output file transcript differential expression testing gives the ratio (log2 of the fold change) of FPKM of a specific transcript between two conditions, which means this fold change in FPKM does not take the overall gene expression into

[galaxy-user] Number of mismatches allowed in the initial read mapping

2012-09-06 Thread Du, Jianguang
Dear All, I tested how to set the Number of mismatches allowed in the initial read mapping as follows. At first, I ran FASTQ Groomer on a dataset to get the number of total reads. The total number of the reads is 17510227. Then I ran Tophat after set Number of mismatches allowed in the