[galaxy-user] SNP Calling

2012-10-17 Thread Francesco Vitiello
Does anyone knows a step-by-step pipeline to SNP calling on illumina dataset? From the alignment to the end thanks Francesco -- ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the

[galaxy-user] Error installing MACS on local Galaxy

2012-10-17 Thread Jerzy Dyczkowski
Hello, I cannot run MACS on the local Galaxy. When I install MACS version 1.4., it gives error something like cannot find macs. When I install version 1.3.7.1 it gives error /line 34, in module from MACS.OptValidator import opt_validate ImportError: No module named MACS.OptValidator/ I

[galaxy-user] How to merge Fastq groomer files in Galaxy?

2012-10-17 Thread Fang,Xiefan
Dear Galaxy users, Does anyone know how to merge several FASTQ groomer files by using Galaxy? If not, is there any other program that can achieve this? The size of one FASTQ groomer file is around 1GB. Thank you! Best regards, Xiefan Fang, Ph.D. Postdoctoral associate Department of

Re: [galaxy-user] How to merge Fastq groomer files in Galaxy?

2012-10-17 Thread Peter Cock
On Tue, Oct 16, 2012 at 8:22 PM, Fang,Xiefan xiefanf...@ufl.edu wrote: Dear Galaxy users, Does anyone know how to merge several FASTQ groomer files by using Galaxy? If not, is there any other program that can achieve this? The size of one FASTQ groomer file is around 1GB. Thank you!

Re: [galaxy-user] FW: problem in data uploading

2012-10-17 Thread Jennifer Jackson
Hello Amit, Are you using the public Main Galaxy instance at http://main.g2.bx.psu.edu (usegalaxy.org) or another server specific to your project? If you are working with a project's Galaxy server, then you will need to contact the administrator running that instance. It sounds as if you

Re: [galaxy-user] cuffdiff values different for same sample

2012-10-17 Thread Jennifer Jackson
Hello, Thank you for sharing your history. The difference in FPKM values can be explained by the use of the -N option (Perform quartile normalization: Yes). Set this to No to avoid the variable per-run normalization. This has also been discussed at seqanswers.com:

[galaxy-user] Export to file

2012-10-17 Thread Dave Corney
Hi list, Is there a currently a known problem with the export to file function? I'm trying to migrate some data from the public galaxy to a private one; the export function worked well with a small (~100mb) dataset, but it has not been working with larger datasets (2GB) and I get the error:

Re: [galaxy-user] cuffcompare input

2012-10-17 Thread Jennifer Jackson
Hello, Yes, this has not changed. The reference GTF given to Cuffdiff must contain all of the transcripts of the inputs: http://user.list.galaxyproject.org/cuffcompare-inputs-td4654384.html Run this as you have done previously:

Re: [galaxy-user] Export to file

2012-10-17 Thread Jennifer Jackson
Hi Dave, Yes, if your Galaxy instance is on the internet, for entire history transfer, you can skip the curl download and just enter the URL from the public Main Galaxy server into your Galaxy directly. To load large data over 2G that is local (datasets, not history archives), you can use

Re: [galaxy-user] Export to file

2012-10-17 Thread Jeremy Goecks
Dave, There's likely something problematic about your history that causing problems. Can you share with me the history that's generating the error? To do so, from the history options menu -- Share/Publish -- Share with a User -- my email address Thanks, J. On Oct 17, 2012, at 6:58 PM,

[galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Hello Everyone. Ctrl+C or Ctrl+D doesn't stop the Galaxy server. In local machine, I need to close the terminal and restart the terminal again. However, in server I integrated several tools, now I need to restart the server to test it. I used ./run.sh --reload doesn't restart the server and I

Re: [galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Enis Afgan
With a certain version of Python there's been an issue stopping Galaxy using ctrl+C. You can use 'sh run.sh --daemon' to have the process run in the background and then 'sh run.sh --stop-damemon' to stop it. Hope this helps, Enis On Thu, Oct 18, 2012 at 4:12 PM, Sachit Adhikari

Re: [galaxy-user] Can't seem to stop or restart the Galaxy in server

2012-10-17 Thread Sachit Adhikari
Thanks. Helped me! On Thu, Oct 18, 2012 at 11:02 AM, Enis Afgan eaf...@emory.edu wrote: With a certain version of Python there's been an issue stopping Galaxy using ctrl+C. You can use 'sh run.sh --daemon' to have the process run in the background and then 'sh run.sh --stop-damemon' to stop