[galaxy-user] Trouble Installing Galaxy on Cluster

2012-11-09 Thread greg
So here is what I've installed so far.  (/usr/local/galaxy) is a
directory that all nodes in the cluster can see.  And the web server
machine can also see it.)

$ls -l /usr/local/galaxy
drwxrwxr-- 20 galaxy  scicomp 2206 Nov  8 15:53 galaxy-dist
-rwxrwxr-x  1 galaxy  scicomp 1882 Nov  8 15:34 galaxy.fedora-init
drwxr-xr-x  5 galaxy scicomp   67 Nov  8 10:08 galaxy_python
-rw-r--r--  1 galaxy scicomp   80 Nov  8 15:32 job_environment_setup_file
drwxrwxr--  2 galaxy  scicomp   28 Nov  8 15:43 logs

Here are are the changes I made to galaxy-dist/universe_wsgi.ini
http://pastie.org/5351347

Here is what I put in my job_environment_setup_file:
export TEMP=/scratch/galaxy
source /usr/local/galaxy/galaxy_python/bin/activate

The galaxy_python directory contains my virtual env based off of Python 2.7.

Finally here are the contents of my galaxy.fedora-init file:
http://pastie.org/5351396

(Note: I created a sym link in /etc/init.d/ to
/usr/local/galaxy/galaxy.fedora-init
Then ran chkconfig --add galaxy.fedora-init
So I can use sudo /sbin/service galaxy.fedora-init start/stop)


Here are the results from running:
$sudo /sbin/service galaxy.fedora-init start
http://pastie.org/5351308

So my first question is why does it appear to be using Python 2.6?  Is
it not using my virtual env?  Am I using virtual env incorrectly?

Thanks,

Greg
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Re: [galaxy-user] [galaxy-dev] Account had be deleted ?

2012-11-09 Thread Nate Coraor
On Nov 7, 2012, at 5:47 AM, Diam Hsu wrote:

 Dear Sir or Madam,
 
 
 
 I used to use Galaxy to analyze NGS data, but these two days I could't login 
 my Galaxy account, it also told me that my account has been marked deleted, 
 and asked me to contact the Galaxy administrator to restore my account.
 
  
 Could you help me restore my accounts because I have to use Galaxy to finish 
 my thesis.

Hi,

Your account was disabled because we discovered that you were using more than 
one account.  The Galaxy Main server is a public resource and we implemented 
quotas to give everyone fair access to our (limited) resources.  If you need 
more resources than we are able to make available on Galaxy Main, your needs 
might be best suited to a cloud or local instance of Galaxy. For more 
information on these, see:

  http://wiki.g2.bx.psu.edu/Big%20Picture/Choices

If you'd like to use one of your existing accounts, please let me know which 
one and I'll re-enable it.

--nate

 
  
 Thank you very much for your help!!!
 
  
 Best regards,
 
  
 Dima Hsu
 
  
 Graduate student,
 
 Graduate Institute of Biomedical Informatics
 
 Taipei Medical University, Taipei, Taiwan
 
 Email:b1984...@gmail.com
 
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Re: [galaxy-user] Identification of replicate outlier

2012-11-09 Thread Dave Corney
Hi Ross,

Thanks for the suggestions. I'm aware that this is not really a
Galaxy-specific question, and I've been browsing through SeqAnswers and
found a couple of suggestions using edgeR or DESeq, but nothing for Tuxedo
suite. However, I have no experience with either of these tools, so I was
wondering how others have approached this problem if their workflow is
based on Cufflinks.

In the meantime, I'll go through your suggestions and see where I get.

Thanks,
Dave


On Thu, Nov 8, 2012 at 7:21 PM, Ross ross.laza...@gmail.com wrote:

 Hi Dave,
 This is an interesting and non-trivial question that extends well
 beyond Galaxy - and there's no simple solution AFAIK
 Defining an 'outlier' tends to boil down to subjective judgement in
 most real cases I've seen.
 EG: see
 http://comments.gmane.org/gmane.science.biology.informatics.conductor/40927

 My 2c worth:
 a) confirm that all of your sample library sizes and quality score
 distributions are comparable with the FastQC tool. A sample with
 relatively low library size may indicate an upstream technical failure
 with (eg) RNA extraction or a flowcell lane.
 b) check that the number of unique alignments to the reference are
 similar (eg picard alignment summary metrics or even the samtools
 flagstat tool)
 c) if you can create an appropriate input matrix (read counts by exon
 or other contig for each sample eg), the Principal Component Analysis
 tool might be helpful (library size normalization is one devil that
 lies in the detail and it's not quite the same as MDS - see below)
 d) If you're an R hacker, you might find

 http://gettinggeneticsdone.blogspot.com.au/2012/09/deseq-vs-edger-comparison.html
 useful - it shows how to get MDS plots which are probably the most
 reliable way to identify samples that don't cluster well with the
 other members of their tribe



 On Fri, Nov 9, 2012 at 10:22 AM, Dave Corney dcor...@princeton.edu
 wrote:
  Hello list,
 
  I've been analyzing an experiment with two groups each with three
  replicates. My workflow was TopHat (paired end) - Cufflinks - CuffDiff.
  Unfortunately, there are not many significant differences identified by
  CuffDiff.
 
  I am wondering whether one of my replicates might be an outlier. Does
  anybody have a suggestion on how to search for an outlier? The quality
  statistics of the unprocessed data looked equally good for all samples,
 so I
  don't think that this is a problem.
 
  Thanks,
  Dave
 
 
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 --
 Ross Lazarus MBBS MPH;
 Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444
 http://scholar.google.com/citations?hl=enuser=UCUuEM4J

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Re: [galaxy-user] Teaching GALAXY

2012-11-09 Thread Larry Reiter

Dannon,

Maybe you can, but not through me.  I'm just a lowly user and 
biologist.  You would have to contact the guy who did the installation 
and runs the server: Lei Yan (leiyan2...@gmail.com)


I would think he would be receptive to some help, so you should mail him.

LTR

On 11/7/12 4:05 PM, Dannon Baker wrote:

Main will complete the jobs eventually, though obviously that's not 
particularly useful in the context of your class today.  How is your cloud 
instance set up?  Cloud or local instances should be the best and most reliable 
option for teaching with and I'd definitely like to figure out why yours failed.

-Dannon


On Nov 7, 2012, at 3:57 PM, Larry Reiter lrei...@uthsc.edu wrote:


Dannon,

Thanks for getting back to me.  Both the local cloud install and the main 
server are frozen.  Well, not exactly frozen.  I can still rename or delete 
jobs, etc.  I can even switch histories and such, but I can't get it to run 
even simple jobs - like taking a demo file and running Fastq Groomer.  I 
realize the load is high today on the main server, but we were able to get jobs 
to run right up until we tried to MAP using Bowtie for Illumina.  At that 
point, all members of the class - and me - could not go any further (i.e. jobs 
are grey and never start to run).  Class ended almost three hours ago.

Does this help?

LTR

On 11/7/12 2:52 PM, Dannon Baker wrote:

Can you describe more about what is broken with your cloud install?

Regarding main - the server has been under heavy load today and, while it 
shouldn't remain frozen, might be slow to dispatch and, depending on the type, 
execute) jobs.

-Dannon

On Nov 7, 2012, at 1:12 PM, Larry Reiter
lrei...@uthsc.edu
  wrote:



Tried to teach a class today on ChipSeq analysis, but the local Cloud install 
is broken and then the Penn server froze on us.  Is there something I should 
know?

LTR

--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-7440 (FAX)
901-448-7443 (Lab)
e-mail:
lrei...@uthsc.edu


Reiter Faculty Page
Reiter Citation Index
Tooth Study
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--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-7440 (FAX)
901-448-7443 (Lab)
e-mail: lrei...@uthsc.edu

Reiter Faculty Page
Reiter Citation Index
Tooth Study



--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-7440 (FAX)
901-448-7443 (Lab)
e-mail: lrei...@uthsc.edu

Reiter Faculty Page http://www.uthsc.edu/neuroscience/faculty/L_Reiter.php
Reiter Citation Index 
http://scholar.google.com/citations?user=-kaQJ14Jhl=en

Tooth Study http://tinyurl.com/88f688l
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Re: [galaxy-user] Teaching GALAXY

2012-11-09 Thread Larry Reiter
It looks like both the main server and our local install are frozen.  
Nothing has run since we started yesterday at about 11:30am.  Anybody 
know why?


LTR

On 11/7/12 4:05 PM, Dannon Baker wrote:

Main will complete the jobs eventually, though obviously that's not 
particularly useful in the context of your class today.  How is your cloud 
instance set up?  Cloud or local instances should be the best and most reliable 
option for teaching with and I'd definitely like to figure out why yours failed.

-Dannon


On Nov 7, 2012, at 3:57 PM, Larry Reiter lrei...@uthsc.edu wrote:


Dannon,

Thanks for getting back to me.  Both the local cloud install and the main 
server are frozen.  Well, not exactly frozen.  I can still rename or delete 
jobs, etc.  I can even switch histories and such, but I can't get it to run 
even simple jobs - like taking a demo file and running Fastq Groomer.  I 
realize the load is high today on the main server, but we were able to get jobs 
to run right up until we tried to MAP using Bowtie for Illumina.  At that 
point, all members of the class - and me - could not go any further (i.e. jobs 
are grey and never start to run).  Class ended almost three hours ago.

Does this help?

LTR

On 11/7/12 2:52 PM, Dannon Baker wrote:

Can you describe more about what is broken with your cloud install?

Regarding main - the server has been under heavy load today and, while it 
shouldn't remain frozen, might be slow to dispatch and, depending on the type, 
execute) jobs.

-Dannon

On Nov 7, 2012, at 1:12 PM, Larry Reiter
lrei...@uthsc.edu
  wrote:



Tried to teach a class today on ChipSeq analysis, but the local Cloud install 
is broken and then the Penn server froze on us.  Is there something I should 
know?

LTR

--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-7440 (FAX)
901-448-7443 (Lab)
e-mail:
lrei...@uthsc.edu


Reiter Faculty Page
Reiter Citation Index
Tooth Study
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--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-7440 (FAX)
901-448-7443 (Lab)
e-mail: lrei...@uthsc.edu

Reiter Faculty Page
Reiter Citation Index
Tooth Study



--
Lawrence T. Reiter, Ph.D.
Associate Professor, Department of Neurology
855 Monroe Ave., Link 415
Memphis, TN 38163

901-448-2635 (Office)
901-448-1160 (FAX)
901-448-7443 (Lab)
e-mail: lrei...@uthsc.edu

Reiter Faculty Page http://www.uthsc.edu/neuroscience/faculty/L_Reiter.php
Reiter Citation Index 
http://scholar.google.com/citations?user=-kaQJ14Jhl=en
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[galaxy-user] (no subject)

2012-11-09 Thread Vevis, Christis
Hi,

I am performing online tophat on 5  different samples which I want to compare 
for gene expression. Is there any simple way, after the end of tophat for all 
of them, with which I can have an excel table with the 5 samples and their hits?

Something similar to this

Vevis1

Vevis2

Vevis3

Vevis4

Vevis5

uc010kuo.1

128.8503

136.60553

146.7073

91.23218

120.325

AK311687

TRA2A

Homo sapiens transformer 2 alpha homolog (Drosophila) (TRA2A), mRNA.

Regards

Kristis Vevis, PhD Student
Cell Biology
UCL Institute of Ophthalmology
11-43 Bath Street
London
EC1V 9EL, UK
020 7608 4067

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Re: [galaxy-user] tophat

2012-11-09 Thread Dannon Baker
It isn't normal, but this can happen during periods of extremely high load like 
we're currently experiencing.  If you leave your jobs in the queue, they'll 
execute as soon as possible - don't cancel or restart your jobs as this will 
only move them to the back of the queue and delay completion.

-Dannon

On Nov 8, 2012, at 5:13 AM, Vevis, Christis christis.vevis...@ucl.ac.uk 
wrote:

 Hi all,
  
 I am trying to perform tophat for illumina from the main server of galaxy and 
 is in queue for 24 hours…is that normal??
  
 Regards
  
 Kristis Vevis, PhD Student
 Cell Biology
 UCL Institute of Ophthalmology
 11-43 Bath Street
 London
 EC1V 9EL, UK
 020 7608 4067
  
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Re: [galaxy-user] Teaching GALAXY

2012-11-09 Thread Dannon Baker
Lei -

It looks like you have two worker nodes, but both are currently listed as 
non-idle, yet have low load.  My guess is that something has gone wrong with 
the SGE configuration causing jobs to simply wait in the queue.  If you look at 
the contents of qconf and qstat on the admin page, does it look like there are 
jobs backed up and/or worker nodes processing the queue?  Depending on how 
comfortable you are working with SGE, it might be easiest to just click 
restart for SGE in the admin panel and see if everything goes back to normal.

-Dannon


On Nov 8, 2012, at 12:43 PM, Lei Yan leiyan2...@gmail.com wrote:

 Dear Dannon,
 
 My name is Lei Yan, and I am a systems administrator at UTHSC.
 I installed a Cloud Galaxy on the Amazon EC2 about two months ago, and most 
 functions work well at that time.
 http://galaxycloud.genenetwork.org/
 But from the day before yesterday, jobs were hung up always. In my opinion, I 
 didn’t find any reasons, because all look correct from Galaxy Admin page (see 
 attachments please).
 Would you please help us to check it? I can give you the administrator 
 password if you need.
 Thanks so much.
 
 
 Lei Yan
 leiyan2...@gmail.com
 UTHSC
 
 
 
 On Wed, Nov 7, 2012 at 4:07 PM, Larry Reiter lrei...@uthsc.edu wrote:
 Dannon,
 
 Maybe you can, but not through me.  I'm just a lowly user and biologist.  You 
 would have to contact the guy who did the installation and runs the server: 
 Lei Yan (leiyan2...@gmail.com)
 
 I would think he would be receptive to some help, so you should mail him.
 
 LTR
 
 On 11/7/12 4:05 PM, Dannon Baker wrote:
 Main will complete the jobs eventually, though obviously that's not 
 particularly useful in the context of your class today.  How is your cloud 
 instance set up?  Cloud or local instances should be the best and most 
 reliable option for teaching with and I'd definitely like to figure out why 
 yours failed.
 
 -Dannon
 
 
 On Nov 7, 2012, at 3:57 PM, Larry Reiter 
 lrei...@uthsc.edu
  wrote:
 
 
 Dannon,
 
 Thanks for getting back to me.  Both the local cloud install and the main 
 server are frozen.  Well, not exactly frozen.  I can still rename or delete 
 jobs, etc.  I can even switch histories and such, but I can't get it to run 
 even simple jobs - like taking a demo file and running Fastq Groomer.  I 
 realize the load is high today on the main server, but we were able to get 
 jobs to run right up until we tried to MAP using Bowtie for Illumina.  At 
 that point, all members of the class - and me - could not go any further 
 (i.e. jobs are grey and never start to run).  Class ended almost three 
 hours ago.
 
 Does this help?
 
 LTR
 
 On 11/7/12 2:52 PM, Dannon Baker wrote:
 
 Can you describe more about what is broken with your cloud install? 
 
 Regarding main - the server has been under heavy load today and, while it 
 shouldn't remain frozen, might be slow to dispatch and, depending on the 
 type, execute) jobs.
 
 -Dannon
 
 On Nov 7, 2012, at 1:12 PM, Larry Reiter 
 
 lrei...@uthsc.edu
 
  wrote:
 
 
 
 Tried to teach a class today on ChipSeq analysis, but the local Cloud 
 install is broken and then the Penn server froze on us.  Is there 
 something I should know?
 
 LTR
 
 -- 
 Lawrence T. Reiter, Ph.D.
 Associate Professor, Department of Neurology
 855 Monroe Ave., Link 415
 Memphis, TN 38163
 
 
 901-448-2635
  (Office)
 
 901-448-7440
  (FAX)
 
 901-448-7443
  (Lab)
 e-mail: 
 
 lrei...@uthsc.edu
 
 
 
 Reiter Faculty Page
 Reiter Citation Index
 Tooth Study
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at 
 usegalaxy.org
 .  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  
 
 http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  
 
 http://lists.bx.psu.edu/
 -- 
 Lawrence T. Reiter, Ph.D.
 Associate Professor, Department of Neurology
 855 Monroe Ave., Link 415
 Memphis, TN 38163
 
 
 901-448-2635
  (Office)
 
 901-448-7440
  (FAX)
 
 901-448-7443
  (Lab)
 e-mail: 
 lrei...@uthsc.edu
 
 
 Reiter Faculty Page
 Reiter Citation Index
 Tooth Study
 
 
 
 -- 
 Lawrence T. Reiter, Ph.D.
 Associate Professor, Department of Neurology
 855 Monroe Ave., Link 415
 Memphis, TN 38163
 
 901-448-2635 (Office)
 901-448-7440 (FAX)
 901-448-7443 (Lab)
 e-mail: lrei...@uthsc.edu
 
 Reiter Faculty Page
 Reiter Citation Index
 Tooth Study
 
 2012-11-07 13-35-01.png2012-11-07 13-36-16.png


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Re: [galaxy-user] (no subject)

2012-11-09 Thread Jeremy Goecks
Kristis,

This data is available further downstream in an RNA-seq analysis pipeline, 
specifically, as output from the Cuffdiff tool. Take a look at the page for 
more details:

https://main.g2.bx.psu.edu/rna-seq

Best,
J.

On Nov 9, 2012, at 3:42 AM, Vevis, Christis wrote:

 Hi,
  
 I am performing online tophat on 5  different samples which I want to compare 
 for gene expression. Is there any simple way, after the end of tophat for all 
 of them, with which I can have an excel table with the 5 samples and their 
 hits?
  
 Something similar to this
 Vevis1
 Vevis2
 Vevis3
 Vevis4
 Vevis5
 uc010kuo.1
 128.8503
 136.60553
 146.7073
 91.23218
 120.325
 AK311687
 TRA2A
 Homo sapiens transformer 2 alpha homolog (Drosophila) (TRA2A), mRNA.
 Regards
  
 Kristis Vevis, PhD Student
 Cell Biology
 UCL Institute of Ophthalmology
 11-43 Bath Street
 London
 EC1V 9EL, UK
 020 7608 4067
  
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  http://lists.bx.psu.edu/

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Re: [galaxy-user] Trouble Installing Galaxy on Cluster

2012-11-09 Thread Scott McManus

I'll take a couple of stabs at this. First, make sure that /scratch/galaxy 
exists.
There's an error at the bottom of your pastie that seems to indicate it's not 
there.
Second, you're right about virtualenv not being used. It is likely that root
is being used, which means that all of the virtualenv stuff for the galaxy user
won't be available. I haven't tried this, but you could try modifying your
galaxy.fedora-init to contain a call to virtualenv - that way you switch into 
the
proper sandbox when you startup.

-Scott

- Original Message -
 So here is what I've installed so far.  (/usr/local/galaxy) is a
 directory that all nodes in the cluster can see.  And the web server
 machine can also see it.)
 
 $ls -l /usr/local/galaxy
 drwxrwxr-- 20 galaxy  scicomp 2206 Nov  8 15:53 galaxy-dist
 -rwxrwxr-x  1 galaxy  scicomp 1882 Nov  8 15:34 galaxy.fedora-init
 drwxr-xr-x  5 galaxy scicomp   67 Nov  8 10:08 galaxy_python
 -rw-r--r--  1 galaxy scicomp   80 Nov  8 15:32
 job_environment_setup_file
 drwxrwxr--  2 galaxy  scicomp   28 Nov  8 15:43 logs
 
 Here are are the changes I made to galaxy-dist/universe_wsgi.ini
 http://pastie.org/5351347
 
 Here is what I put in my job_environment_setup_file:
 export TEMP=/scratch/galaxy
 source /usr/local/galaxy/galaxy_python/bin/activate
 
 The galaxy_python directory contains my virtual env based off of
 Python 2.7.
 
 Finally here are the contents of my galaxy.fedora-init file:
 http://pastie.org/5351396
 
 (Note: I created a sym link in /etc/init.d/ to
 /usr/local/galaxy/galaxy.fedora-init
 Then ran chkconfig --add galaxy.fedora-init
 So I can use sudo /sbin/service galaxy.fedora-init start/stop)
 
 
 Here are the results from running:
 $sudo /sbin/service galaxy.fedora-init start
 http://pastie.org/5351308
 
 So my first question is why does it appear to be using Python 2.6?
  Is
 it not using my virtual env?  Am I using virtual env incorrectly?
 
 Thanks,
 
 Greg
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