[galaxy-user] preprocessing gDNA illumina paired end data for mapping/snp calling

2013-04-24 Thread Timothy Brennan
This question is w/ regards to pre-processing whole genome resequencing data for mapping data to a reference yeast strain. I'm having trouble joining paired end data. I have two files per sample (read1 and read2). I've successfully uploaded my fastq.gz files into galaxy using FTP. I have two

[galaxy-user] ImportError: Unable to run arch-specific checks

2013-04-24 Thread Shun Liang
Dear Galaxy Developers, I am trying to run Galaxy on ARM architecture. I am able to scramble the eggs locally. However, I am unable to run Galaxy on my machine as run.sh gives an error after some initialization steps: galaxy@arm01-48:/mnt/ceph/galaxy/galaxy-dist$ ./run.sh Initializing

Re: [galaxy-user] preprocessing gDNA illumina paired end data for mapping/snp calling

2013-04-24 Thread Jennifer Jackson
Hello Tim, Yes, this is a known issue, a few of the tools do not function with sequences with the newer formatted identifiers. There is a bare-bones Trello ticket for the upgrade, but to be honest, this hasn't been prioritized because of so few use cases: https://trello.com/c/bhurghHk

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread Dave Clements
Hi David, Using Galaxy to teach undergraduates is a long term interest of mine. Which, unfortunately, does not mean I have yet put a lot of thought into it. However, lack of thought hasn't stopped me yet. First, this topic was discussed in a breakout at last year's GCC:

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread David Joly
Thanks Dave! The focus is clearly more like your second description. The course is not intended to show them how to use command lines, or how to navigate in a UNIX environment and how to program. In fact, I think some would call what I have in mind more of a computational biology course than a

Re: [galaxy-user] [galaxy-dev] Teaching using Galaxy

2013-04-24 Thread Dave Clements
Hi David, Galaxy does sound like a great match for this course. It could also play a (smaller) part in the other course you are considering. Part of the course could include installing Galaxy and wrapping other tools to put into it. We are a Python based framework I do strongly recommend you