Re: [galaxy-user] SNP finding

2013-11-11 Thread Mark Lindsay
Dear Galaxy UsersI wondered if anybody is having the same problem.I am trying to run CuffDiff using the latest version of GenCode18 and keep getting the following error message.Is there a problem with the network or is it me?Best wishesMark___
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Re: [galaxy-user] SNP finding

2013-11-11 Thread Jennifer Jackson

Hello Xiefan,

This page has a simple breakdown of how to call variants within Galaxy. 
This is brand new, so full annotation/video is pending, but it should 
still be straightforward to see how the data is prepped and which tools 
are used.

https://usegalaxy.org/u/galaxyproject/p/galaxy-101-ngs-variant

Another set of tutorial videos cover the tools, which you can use on the 
public site or on a scaled up cloud server as needed:

https://vimeo.com/channels/galaxytoolshed

Hopefully this help you to get started.

Jen
Galaxy team

On 10/21/12 3:11 PM, Xiefan Fang wrote:

Dear galaxy users,
We have done deep sequencing on some known genomic loci using 
Hiseq2000. I have already mapped the reads to the reference sequences 
by using Galaxy. In the next step, I want to find SNPs and calculate 
the SNP percentage within the reads. There are 500,000 to 1,000,000 
reads per biological sample. Can I do it with galaxy? If not, is there 
other programs available in windows? Considering that I am not very 
familiar with programming.


Thanks,
 Xiefan
University of Florida


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--
Jennifer Hillman-Jackson
http://galaxyproject.org

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Re: [galaxy-user] SNP finding

2013-11-11 Thread Nate Coraor
Hi Mark,

This error is not tool related.  Could you click the “bug” icon to send us an 
error report?

—nate

On Nov 11, 2013, at 8:33 AM, Mark Lindsay m.a.lind...@bath.ac.uk wrote:

 Dear Galaxy Users
 
 I wondered if anybody is having the same problem.
 
 I am trying to run CuffDiff using the latest version of GenCode18 and keep 
 getting the following error message.
 
 Is there a problem with the network or is it me?
 
 Best wishes
 
 Mark
 
 
 
 
 Screen Shot 2013-11-11 at 13.32.12.png
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
 http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
 http://lists.bx.psu.edu/
 
 To search Galaxy mailing lists use the unified search at:
 
 http://galaxyproject.org/search/mailinglists/


___
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