Re: [galaxy-user] Random Intervals ?

2011-12-10 Thread Vincent Joseph Lynch
Hi Jen,

Thanks for getting back to me. I'm not sure whether selecting random lines will 
work but will give it a try. 

If such a tool is ever built in the future it would be nice if it could match a 
file that has empirical data to generate a null expectation. For example, I 
have a bed file with the location of features that are of variable length and 
these features tend to be associated with particular genes. So randomly 
sampling the genome n times equal to the feature length from the original bed 
file should give some idea of a random expectation for the association of the 
feature (ChIp-Seq peaks or the like) with other features (genes or the like).

Thanks again for the quick reply!
Vinny




On Dec 7, 2011, at 8:56 AM, Jennifer Jackson wrote:

 Hello Vinny,
 
 The tool Text Manipulation - Select random lines from a file may be of 
 interest to you. This will not generate random intervals, but it can select 
 random lines from an interval file or any other file.
 
 The ENCODE tool as build specifically on the target genomes using external 
 files. This tool may be generalized at some point in the future, but right 
 now there are no current plans to do so.
 
 Hopefully the random line tool will be useful or you will be able to locate 
 an alternative,
 
 Best,
 
 Jen
 Galaxy team
 
 On 11/29/11 12:43 PM, Vincent Joseph Lynch wrote:
 To Whom It May Concern,
 
 I am curious if there is a tool within Galaxy to generate a set of
 random intervals from a particular genome similar to the Random
 Intervals tool within the ENCODE tools? I am using the Aggregate
 datapoints tool to get phastCons conservation scores for peaks from
 ChIP-Seq data. I would like to compare these scores to a random
 expectation so would like to be able to use a Random Intervals-like tool
 to generate a set of random positions to compare to the experimental set.
 
 Best,
 Vinny
 
 
 
 
 Vincent J. Lynch, Associate Research Scientist
 Department of Ecology and Evolutionary Biology  Yale Systems Biology
 Institute
 Yale University
 
 There is a grandeur in this view of life, with its several powers,
 having been originally breathed into a few forms or into one; and that
 whilst this planet has gone on cycling according to the fixed laws of
 gravity, from so simple a beginning endless forms most beautiful and most
 wonderful have been, and are being, evolved. -C. Darwin, 1859
 
 
 
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Vincent J. Lynch, Associate Research Scientist
Department of Ecology and Evolutionary Biology  Yale Systems Biology Institute
Yale University
 
There is a grandeur in this view of life, with its several powers,
having been originally breathed into a few forms or into one; and that
whilst this planet has gone on cycling according to the fixed laws of
gravity, from so simple a beginning endless forms most beautiful and most
wonderful have been, and are being, evolved. -C. Darwin, 1859

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[galaxy-user] Conservation (i.e. PhastCons) Q

2012-08-01 Thread Vincent Joseph Lynch
Hi All,

I am using Galaxy (main) and would like to intersect a bed file I have with the 
PhastCons scores (primarily placental and primate). After much searching around 
I can't tell if these files are locally cached and if so how I access them. 
Alternatively I could get them directly from UCSC, but it seems like these 
files would be rather large and useful to other users as well. (I apologize if 
I am missing the obvious, I'm still a novice user.)

I am using hg19, but of course could lift over to convert the coordinates. 

Best,
Vinn



Vincent J. Lynch, Ph.D. 
Associate Research Scientist
Department of Ecology and Evolutionary Biology (EEB)  Yale Systems Biology 
Institute (YSBI)
Yale University
 
There is a grandeur in this view of life, with its several powers,
having been originally breathed into a few forms or into one; and that
whilst this planet has gone on cycling according to the fixed laws of
gravity, from so simple a beginning endless forms most beautiful and most
wonderful have been, and are being, evolved. -C. Darwin, 1859




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Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
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use the Galaxy Development list:

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