[galaxy-user] filtering pile-up fails

Dear All, I have been successful by using the online tool to align Illumina pair end reads , each direction 5GB, and also generated a pileup of 680,000,000 lines, but the filtering of the pileup always fails, it runs for several hours and I get an empty file back. I tried different options and

Re: [galaxy-user] filtering pile-up fails

Hello Sebahattin, It may be that the data is too large to run on the Galaxy public instance, which means that a local or cloud instance would be the next recommendation, as explained in this wiki: http://wiki.g2.bx.psu.edu/Big%20Picture/Choices But, before you make the investment of moving