Re: [galaxy-user] Cufflinks quartile normalization

2011-08-27 Thread Jeremy Goecks
=== Please use Reply All when responding to this email! === David, Quartile normalization is explained in the Cufflinks manual: http://cufflinks.cbcb.umd.edu/manual.html -- With this option, Cufflinks normalizes by the upper quartile of the number of fragments mapping to individual loci

[galaxy-user] Cufflinks quartile normalization

2011-08-25 Thread David Joly
=== Please use Reply All when responding to this email! === Can someone help me understanding the quartile normalization in Cufflinks? I read different threads in which they reported that the FPKM values were inflated after normalization (-N) but most people didn't report their values so I don't