Subject: Re: [galaxy-user] Please help me check the quality of the Tophat
mapping to reference genome
Hello Jianguang,
This is the expected output from this particular tool. Your TopHat
output file 'accepted hits' contains only mapped data.
I did notice this option for the TopHat run:
Hello Jianguang,
This is the expected output from this particular tool. Your TopHat
output file 'accepted hits' contains only mapped data.
I did notice this option for the TopHat run:
> Is this library mate-paired?
> Single-end
Your data was originally paired end - so this is unexpected. But
Dear All,
I ran "Flagstat" under "NGS: SAM Tools" to check the quality of the Tophat
output (the file of accepted hits). I got the diagnosis results as follow:
9471730 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 duplicates
9471730 + 0 mapped (100.00%:-nan%)
0 + 0 paired in sequencing
3 matches
Mail list logo