[galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Felix Mayr
Hi there, Looking at the output of the SplicingDiff files of CuffDiff, me and my colleagues are preplexed about the output of the p_values and q_values. We've tried different inputs of different samples to compare but never seem to manage to get p_values smaller than 0.50 and we keep getting

Re: [galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Jeremy Goecks
Felix, You seem to be providing the correct inputs to Cuffdiff and it appears to be producing valid output. More information about setting parameter values and interpreting Cuffdiff can be found in manual: http://cufflinks.cbcb.umd.edu/manual.html#cuffdiff Good luck, J. On Jun 16, 2011, at

Re: [galaxy-user] Question about output CuffDiff SplicingDiff

2011-06-16 Thread Rory Kirchner
What version of Cufflinks is your Galaxy installation running? A recent version (1.00 and 1.01) had a problem that was causing the splicing and promoter use tests to have very few differentially regulated genes, according to http://cufflinks.cbcb.umd.edu/. -rory On Jun 16, 2011, at 8:13 AM,