Re: [galaxy-user] Analyzing Targeted Resequencing data with Galaxy

2011-04-08 Thread Mike Dufault
Sean, Anton and Jen,   Thanks for all of the suggestions (in separate replies) on how to better analyze my SelectSure captured Exome data. My original work-flow is below in the e-mail string.   Based on the suggestions, I plan to change my work-flow by increasing my quality filter from 20 to

Re: [galaxy-user] Analyzing Targeted Resequencing data with Galaxy

2011-04-08 Thread Anton Nekrutenko
Mike: Realignment and recalibration is not yet possible on the main site. However, we are working on several re-sequencing projects in house where these tools are used and will bring them to Galaxy by ISMB conference in Vienna. The indel analysis at the moment is rather simplistic (yet still

Re: [galaxy-user] RNA seq analysis and GTF files

2011-04-08 Thread David K Crossman
Jeremy, Thank you very much for this information. One quick question. I added the gene_id values to the 10th column of my patched GTF file. After uploading it to Galaxy, the column doesn't have a name (i.e. column 1 = Seqname; column 2 = Source; etc...). Do I need to assign