Re: [galaxy-user] Ngs Mapping using LASTZ

2011-07-29 Thread Thomas Jenner Pulikotial Augustine
Hi, I'm trying to map a data set containing 454 reads to a reference genome using LASTZ alignment package in Galaxy. I'm following a workflow used for Illumina reads with the only change in the alignment package, i'm using LASTZ instead of bowtie/bwa. The following is the procedure please correct

[galaxy-user] renaming dataset in workflow

2011-07-29 Thread Belinda M. Giardine
Is it possible when renaming a dataset in a workflow to have it fill in the dataset number like the default labels? For example the default label may be "Filter on data 17". What if I want it to be "High coverage on data 17"? Could I put some special variable in the text to tell Galaxy to fi

Re: [galaxy-user] renaming dataset in workflow

2011-07-29 Thread Dannon Baker
While this is not currently possible, we have recently discussed exposing extra fields for use in both parameters and rename actions. If you're interested, I've created a bitbucket enhancement request (https://bitbucket.org/galaxy/galaxy-central/issue/623/expose-more-fields-to-rename-action-and

Re: [galaxy-user] Ngs Mapping using LASTZ

2011-07-29 Thread Jennifer Jackson
Hello Thomas, For 454 data, there is a specific screencast that may be of interest. Go to http://usegalaxy.org and scroll in the middle pane to find quickie #15. We have had very high usage over the last few days, but have since been able to clear out more resource. Your job will likely start

Re: [galaxy-user] question about using bowtie

2011-07-29 Thread Jennifer Jackson
Hello William, The tools in "NGS: QC and manipulation", especially those in the sub-section "AB-SOLiD data" can do the manipulations needed before mapping. It may be helpful to view the screencast at http://usegalaxy.org, center pane, quickie #9. Hopefully this helps to get you started, Bes

Re: [galaxy-user] generating a new fasta from a pileup

2011-07-29 Thread Jennifer Jackson
Hello David, Generating a consensus fasta sequence from a BAM or Pile-up file is not yet possible in Galaxy. To date, the Tool Shed also does not have a wrapped/novel tool for this function either. If you or another user were to create such a wrapped tool, it would be most welcome. As would

[galaxy-user] Gene fusion version of Tophat in Galaxy

2011-07-29 Thread vasu punj
Jen/ Jeremy/ Galaxy team,   Is there any plan of implementing gene fusion version of TopHat in Galaxy perhaps in next few months or so? Alternatively, if some one has used with gene fusion using TopHat in Galaxy frame work please share.   Thanks.   Vasu Punj___