[galaxy-user] (no subject)

2011-09-28 Thread raghava rao
-- Y.P.V.S.Raja Raghava Rao Research Scholar Dr.K.Sreenivasulu Lab Dept.of Animal Sciences School of Life Sciences University of Hyderabad ypvs...@gmail.com 9989733698 ___ The Galaxy User list should be used for the discussion of Galaxy

Re: [galaxy-user] run tophat in galaxy

2011-09-28 Thread Peng, Tao
Jen, thank you for following up on my question. Is any tool in GALAXY to visualize the coverage of aligned reads from TopHat on human chromosomes (histogram or density plot)? Tao -Original Message- From: Jennifer Jackson [mailto:j...@bx.psu.edu] Sent: Thursday, September 15, 2011 2:37

Re: [galaxy-user] Galaxy and Ion Torrent

2011-09-28 Thread Peter Cock
2011/9/28 cuentasecuenciacion secuenciac...@ipb.csic.es: Hello!! Does anybody know whether Ion Torrent data is supported by Galaxy? Thank you!! IonTorrent data acts a lot like Roche 454, it even comes as SFF files (or FASTQ). So in principle anything Galaxy supports for 454 would be worth

Re: [galaxy-user] Galaxy and Ion Torrent

2011-09-28 Thread Anton Nekrutenko
Also, test instance (http://test.g2.bx.psu.edu) has TMap mapper, which was recently rolled out for testing. The wrapper was contributed by Nils Homer. At this time there are only E. coli indices, but more can be added. a. Anton Nekrutenko http://galaxyproject.org On Sep 28, 2011, at 10:18

Re: [galaxy-user] Problem in merging two BAM file in Galaxy. Thanks for help.

2011-09-28 Thread Jennifer Jackson
Hello Bin, The tools under NGS: Picard (beta) - BAM/SAM Cleaning can help to label the reads with the same identifier in the two SAM files appropriately so that they do not trigger this type of error. Specifically, the reads need to be assigned to different run groups. Hopefully this helps,

[galaxy-user] Indel analysis

2011-09-28 Thread Golbus, Jessica
Hi- I am using Galaxy for Indel analysis. Does the Indels from Sam analysis use Dindel to pull variation? Thanks! ** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues

Re: [galaxy-user] Indel analysis

2011-09-28 Thread Anton Nekrutenko
Jessica: No, dindel is not yet a part of Penn State Galaxy Instance. anton Anton Nekrutenko http://galaxyproject.org On Sep 28, 2011, at 12:21 PM, Golbus, Jessica wrote: Hi- I am using Galaxy for Indel analysis. Does the Indels from Sam analysis use Dindel to pull variation?

Re: [galaxy-user] BED to BAM conversion in Galaxy

2011-09-28 Thread Jennifer Jackson
Hello, It is possible to go from SAM/BAM to BED, but not the reverse. SAM/BAM files contain the actual sequence data associated with the original aligned read. BED files only have the reference genome location of the alignment (no read sequence). It is possible to extract genomic sequence

[galaxy-user] Adding new genomes to Galaxy

2011-09-28 Thread Jennifer Jackson
Hello James, I'll post this to the galaxy-user list for you. Next time, just send an email to galaxy-u...@bx.psu.edu to post a question/request. GMOD supports a searchable archive, but it is not a forum. You can follow the discussion there or join the mailing list to receive content:

Re: [galaxy-user] Gro-seq! and Galaxy

2011-09-28 Thread Jennifer Jackson
Hello, One quick guess is that the file extensions did not end with a .bed or a .wig ? But, this is easy to assign. Click on the pencil icon in the top right corner of the dataset to reach the Edit Attributes form. Here you can set the datatype manually and the appropriate metadata will be