[galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Noa Sher
The correct link: http://www.microbesonline.org/cgi-bin/genomeInfo.cgi?tId=59919 Previous mail: I am a non-programmer working on  Prochlorococcus (a marine bacteria) for which UCSC and Ensembl do not yet have genome/transcriptome available or uploaded.  Howe

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
Good Morning Noa: Which one of the files at microbesonline are you trying to work with ? --Hiram - Original Message - From: "Noa Sher" To: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 1:38:35 AM Subject: [galaxy-user] there was a wrong link in my previous mail - gtf file

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Noa Sher
Hi Hiram, I was trying to work with the tab delineated file (using the link under export genomic data). Thanks noa On 04/12/2011 20:29, Hiram Clawson wrote: Good Morning Noa: Which one of the files at microbesonline are you trying to work with ?

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
There are at least six of them there. Which one ? - Original Message - From: "Noa Sher" To: "Hiram Clawson" Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re: [galaxy-user] there was a wrong link in my previous mail - gtf fileissues Hi Hi

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
Is this the genome you are working with: http://archaea.ucsc.edu/cgi-bin/hgGateway?db=procMari_CCMP1375 - Original Message - From: "Noa Sher" To: "Hiram Clawson" Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re: [galaxy-user] there was a wrong li

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
Pardon me, I see there is only one that says "tab-delimited" file. That is a tough one to decode. It almost looks like GTF already, but not quite. If we take it as a simple file of annotations on the genome, without structure such as exons, introns, and merely rework the columns to turn it into

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Noa Sher
Hi Hiram, I managed to extract the columns in a different order (albeit I did it in excel and not using command line) but then the 9th column (attributes) of gtf is what I had problems with Thanks noa On 04/12/2011 21:43, Hiram Clawson wrote:

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
For your full 9-column GTF file, it is, as you have determined, tab-delimited for the 9 columns. In the ninth you can place any value tag pairs with semi-colon ; delimiter. Beware your excel produced file does not have hidden characters that can upset the balance. View your file in an editor tha

[galaxy-user] Sort sam or bam

2011-12-04 Thread Clare Sloggett
Hi galaxy users, Am I right in thinking there is no tool for sorting a sam/bam file in Galaxy? I think this has probably been discussed before, sorry. I just want to check I haven't missed anything, since sibling tools from e.g. the samtools and picard suites are wrapped. Thanks, Clare -- E: s