[galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Elwood Linney
After successfully using RNAseq software in Galaxy online for about 10 different datasets to just get gene expression differences between replicates from control versus exposed zebrafish embryos, I am having no luck getting cuffdiff to work with the "moved" Galaxy. I had this problem with histor

Re: [galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Jeremy Goecks
Are you reporting a bug for each failed Cuffdiff run? That's the easiest way for the Galaxy team to help you out. One thing to keep in mind is that, for now, spaces are not allowed in condition names. We'll address this problem soon. Best, J. On Oct 22, 2013, at 5:42 AM, Elwood Linney wrote:

Re: [galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Jennifer Jackson
Elwood, Would you please share: 1. an original history that worked (if possible) 2. the workflow you are using and let me know how you generated (did you extract it recently (last week or so)? 3. problem history There are a few known issues in here, some mitigated, one that has to do with ex

Re: [galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Nate Coraor
Hi Elwood, Jeremy and I took at a look at this. The failures in your history with this message: Error: number of labels must match number of conditions ...are due to a regression in the cuffdiff tool which Jeremy has just fixed. Some characters such as spaces in the condition name were val

Re: [galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Elwood Linney
thank you all, its clearly working now and that is coming just in time, the results of two of these datasets will help us plan our next experiments for capping up a manuscript or two el linney On Tue, Oct 22, 2013 at 12:03 PM, Nate Coraor wrote: > Hi Elwood, > > Jeremy and I took at a look at

Re: [galaxy-user] problems in transitioning from Tophat to Cuffdiff

2013-10-22 Thread Nate Coraor
This is great news, thanks for letting us know. --nate On Oct 22, 2013, at 2:34 PM, Elwood Linney wrote: > thank you all, its clearly working now and that is coming just in time, the > results of two of these datasets will help us plan our next experiments for > capping up a manuscript or two