Re: [galaxy-user] get wig file after tophat

2011-02-21 Thread Hiram Clawson
FYI: samtools + bam file + a bit of perl == to wiggle conversion: https://lists.soe.ucsc.edu/pipermail/genome/2010-December/024318.html - Original Message - From: Ying Zhang ying.zhang.yz...@yale.edu To: galaxy-u...@bx.psu.edu Sent: Monday, February 21, 2011 12:39:47 PM Subject:

Re: [galaxy-user] Pseudo Autosomal regions in Chrs X and Y

2011-03-28 Thread Hiram Clawson
Listed on the hg19 gateway page at the UCSC genome browser. - Original Message - From: David Matthews d.a.matth...@bristol.ac.uk To: Jennifer Jackson j...@bx.psu.edu Cc: galaxy-u...@bx.psu.edu Sent: Monday, March 28, 2011 2:04:02 PM Subject: Re: [galaxy-user] Pseudo Autosomal regions in

Re: [galaxy-user] [Genome] UCSC browser, access from Galaxy

2011-03-31 Thread Hiram Clawson
Good Morning Dr. Hunt: Can you please clarify what upload function from galaxy you are trying to perform ? Perhaps the galaxy user help email list could direct you to advice on this subject ? (copied on this email) The Ensembl genome browser accepts the same types of upload tracks as does the

Re: [galaxy-user] bed to bigbed conversion

2011-06-19 Thread Hiram Clawson
FYI: The bedToBigBed converter is one of the lower memory usage big* converters, with a longer running time: http://genomewiki.ucsc.edu/index.php/Big_file_converters --Hiram ___ The Galaxy User list should be used for the discussion of

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
There are at least six of them there. Which one ? - Original Message - From: Noa Sher noa.s...@gmail.com To: Hiram Clawson hi...@soe.ucsc.edu Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re: [galaxy-user] there was a wrong link in my previous mail

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
Is this the genome you are working with: http://archaea.ucsc.edu/cgi-bin/hgGateway?db=procMari_CCMP1375 - Original Message - From: Noa Sher noa.s...@gmail.com To: Hiram Clawson hi...@soe.ucsc.edu Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re

Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues

2011-12-04 Thread Hiram Clawson
with the format. I can't see immediately how to use it properly. --Hiram - Original Message - From: Noa Sher noa.s...@gmail.com To: Hiram Clawson hi...@soe.ucsc.edu Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re: [galaxy-user] there was a wrong link in my

Re: [galaxy-user] Uploading Genome from Ensembl

2011-12-06 Thread Hiram Clawson
Good Afternoon Sheena: Can you please explain what is different between the Ensembl and UCSC Zebrafish Zv9 genome sequences ? --Hiram On 11/4/11 2:50 PM, Scroggins, Sheena wrote: How do I upload the Zebrafish genome from Ensembl to my user history in Galaxy? I'm trying to map my RNA-Seq data

Re: [galaxy-user] Displaying bed files in ucsc

2012-05-11 Thread Hiram Clawson
Good Evening Jose: Please note the types of graphing formats you can display at UCSC: http://genomewiki.ucsc.edu/index.php/Selecting_a_graphing_track_data_format I would recommend converting your bedgraph file to a bigWig file and display that via a remote URL. This would be your most

Re: [galaxy-user] Displaying bed files in ucsc

2012-05-11 Thread Hiram Clawson
Please note the arguments you can use in a track line for a type=wiggle_0 format file: http://genome.ucsc.edu/goldenPath/help/wiggle.html The arguments useScore, scoreMin, scoreMax have no meaning with a wiggle track. A wiggle track does not display items in a gray-scale fashion, it draws a

Re: [galaxy-user] Problem with Depth of Coverage on BAM files (GATK tools)

2012-06-18 Thread Hiram Clawson
I'm curious what is this genome called 'hg_g1k_v37' and how does it correspond to NCBI GRCh37 which is identical to UCSC hg19 ? --Hiram Jennifer Jackson wrote: UCSC does not contain the genome 'hg_g1k_v37' - the genome available from UCSC is 'hg19'. Even though these are technically the

[galaxy-user] data expiration policy

2012-10-05 Thread Hiram Clawson
Good Morning Galaxy Universe: What is the data expiration policy at Galaxy for user created big* files that are then subsequently used in custom tracks at UCSC ? We are noticing users with bigDataUrl paths of: https://main.g2.bx.psu.edu/display_application/.../somefile.bigwig which appear to

Re: [galaxy-user] display at ucsc error

2013-09-12 Thread Hiram Clawson
Please note, this sequence chrM_rCRS does not exist in the human/hg19 genome browser at UCSC. It is not a recognized sequence. There is a note about this on the gateway page: http://genome.ucsc.edu/cgi-bin/hgGateway?db=hg19 See the paragraph titled: Note on chrM --Hiram On 9/12/13 1:25 AM,